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Method for analyzing nicotine chiral isomers in tobacco and smoke

A technology for chiral isomers and analytical methods, which is applied in the directions of analytical materials, material separation, and measurement devices to achieve the effect of eliminating impurity interference, simple and fast analytical methods, and good repeatability.

Active Publication Date: 2017-06-23
SHANGHAI TOBACCO GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the chiral analysis of nicotine in the smoke of tobacco raw materials, cigarettes, e-cigarette liquids, heat-not-burn cigarettes and the above substances by convergence chromatography

Method used

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  • Method for analyzing nicotine chiral isomers in tobacco and smoke
  • Method for analyzing nicotine chiral isomers in tobacco and smoke
  • Method for analyzing nicotine chiral isomers in tobacco and smoke

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1. Sample pretreatment

[0062] Take liquid e-cigarette liquid and add a mixed solution of n-hexane and isopropanol to dilute to obtain the liquid to be tested. Wherein, in the mixed solution of n-hexane and isopropanol, the volume ratio of n-hexane and isopropanol added is 7:3. The volume ratio of the e-cigarette liquid to the mixed solution of n-hexane and isopropanol is 1:10-100.

[0063] 2. Preparation of standard solution

[0064] Weigh a certain amount of racemic nicotine standard substance, the mass ratio of S-(-)-nicotine and R-(+)-nicotine in the racemic nicotine standard substance is 1:1, using n-hexane Dissolve the mixed solution with isopropanol and make it to volume, and first make a standard stock solution. Then pipette different amounts of standard stock solutions, dilute to volume with a mixed solution of n-hexane and isopropanol to obtain a series of standard solutions with different concentrations. The concentration range of S-(-)-nicotine in the s...

Embodiment 2

[0075] 1. Sample pretreatment

[0076] Take liquid e-cigarette liquid and add a mixed solution of n-hexane and isopropanol to dilute to obtain the liquid to be tested. Wherein, in the mixed solution of n-hexane and isopropanol, the volume ratio of n-hexane and isopropanol added is 6-8:2-4. The volume ratio of the e-cigarette liquid to the mixed solution of n-hexane and isopropanol is 1:0-1000.

[0077] 2. Preparation of standard solution

[0078] Weigh a certain amount of racemic nicotine standard substance, the mass ratio of S-(-)-nicotine and R-(+)-nicotine in the racemic nicotine standard substance is 1:1, using n-hexane Dissolve the mixed solution with isopropanol and make it to volume, and first make a standard stock solution. Then pipette different amounts of standard stock solutions, dilute to volume with a mixed solution of n-hexane and isopropanol to obtain a series of standard solutions with different concentrations. The concentration range of S-(-)-nicotine in t...

Embodiment 3

[0089] 1. Sample pretreatment

[0090] Take a solid tobacco sample as raw material for tobacco. The raw material for tobacco is selected from any one of tobacco leaves, tobacco stems, tobacco flakes or shredded tobacco. Any one of tobacco leaves, oriental tobacco leaves, and flue-cured tobacco leaves. After grinding the solid tobacco sample, take 1g of the sample and put it in a 15mL test tube, add 2mL of 10% NaOH aqueous solution and 4mL of methyl tert-butyl ether, screw the cap tightly, shake it fully for 5min, and let it stand at room temperature 20-25°C 22-26h overnight. The supernatant was filtered through a 0.45 μm filter membrane and then diluted with a mixed solution of n-hexane and isopropanol to obtain the test solution. Wherein, in the mixed solution of n-hexane and isopropanol, the volume ratio of n-hexane and isopropanol added is 7:3. The volume ratio of the supernatant to the added supernatant is 1:0-10. Samples were measured in parallel three times.

[0091...

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Abstract

The invention provides a method for analyzing nicotine chiral isomers in tobacco and smoke. The method specifically comprises the following steps: (1) pretreating a sample: treating the tobacco sample, so as to obtain a solution to be detected; (2) preparing a standard solution; (3) carrying out qualitative detection on the sample: carrying out ultrahigh-performance bonded-phase chromatography detection on the solution to be detected and the standard solution separately, comparing retention time to carry out qualitative detection, and determining ingredients of the nicotine chiral isomers in the solution to be detected; (4) carrying out quantitative detection on the sample: carrying out ultrahigh-performance bonded-phase chromatography detection on the solution to be detected and the standard solution separately, and carrying out quantification by adopting an external standard method, so as to obtain the content of the nicotine chiral isomers in the solution to be detected. According to the method for analyzing the nicotine chiral isomers in the tobacco and smoke, provided by the invention, nicotine serves as an object of study, liquid, solid and gaseous tobacco samples are pretreated, and ultrahigh-performance bonded-phase chromatographic conditions are optimized, so that the sensitivity is high, the repeatability is good, and the accurate determination of composition of the nicotine chiral isomers can be achieved.

Description

technical field [0001] The invention belongs to the field of analysis of chemical components of tobacco, and relates to a method for analyzing chiral isomers of nicotine in tobacco and flue gas, in particular to raw materials for tobacco, cigarettes, e-cigarette liquid, heat-not-burn cigarettes and the above-mentioned substances An ultra-high performance convergence chromatography method for the analysis of chiral isomers of nicotine in flue gas. Background technique [0002] Nicotine is the most abundant alkaloid in tobacco and smoke, which can act on the central nervous system of the human body and is the main driving force for smokers to smoke. Nicotine contains a chiral center, and there are two enantiomers: S-(-)-nicotine and R-(+)-nicotine, which are completely different in content, metabolic mechanism and physiological characteristics in tobacco. Nicotine in natural tobacco is mainly in the S-(-)-configuration, but through high-sensitivity analysis techniques, R-(+)-...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 费婷吴达刘百战
Owner SHANGHAI TOBACCO GRP CO LTD
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