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Potamogeton malaianus-sodium alginate pellet-type algistat as well as preparation method and application thereof

A technology of kohlrabi and sodium alginate, which is applied in the direction of botany equipment and methods, applications, herbicides and algicides, etc., can solve the problems of short duration and uneven distribution of algae-inhibiting substances, and achieve easy Excellent performance in degradation and algae inhibition, and high inhibition efficiency

Inactive Publication Date: 2017-05-10
NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the problems of uneven concentration distribution and short duration of the existing algae-inhibiting substances, the present invention provides an algae-inhibiting agent, Sodium Alginate and its preparation method and application, which can effectively avoid submerged water Plant colonization and later management are difficult, and the effect of water diffusion on the concentration of algae-inhibiting substances provides an effective and feasible method for water purification and inhibition of algae growth, which can be applied to the control and suppression of algae in lake blooms

Method used

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  • Potamogeton malaianus-sodium alginate pellet-type algistat as well as preparation method and application thereof
  • Potamogeton malaianus-sodium alginate pellet-type algistat as well as preparation method and application thereof
  • Potamogeton malaianus-sodium alginate pellet-type algistat as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation steps of kohlrabi powder-sodium alginate pellets are as follows:

[0041] Step 1: Wash the scallops collected from the aquatic plant breeding base, and use cuttings to plant the washed scallops in a tank covered with bottom mud and sterilized by ultraviolet light. For 2 weeks, during the cultivation period, the temperature was controlled at 28°C, the light was 3000lx, and the light-dark cycle was 12:12h.

[0042] Step 2: After the cultivation is over, take out the coriander, wash it with sterile water, and put it in an oven for 48 hours at 80°C. After drying, grind it into powder and sieve it with a 150-mesh sieve .

[0043] Step 3: Weigh 50g of the sieved powder into a 1L Erlenmeyer flask, add 500mL of sterile water, place in a shaker, and extract at 25°C for 2 days.

[0044] The fourth step: after the extraction, the extract was centrifuged at 3500r / min for 15min, and then filtered with a glass fiber membrane (GF / F membrane) with a pore size of 25mm ...

Embodiment 2

[0050] The preparation steps of kohlrabi powder-sodium alginate pellets are as follows:

[0051] Step 1: Wash the scallops collected from the aquatic plant breeding base, and use cuttings to plant the washed scallops in a tank covered with bottom mud and sterilized by ultraviolet light. For 3 weeks, during the cultivation period, the temperature was controlled at 30°C, the light was 2500lx, and the light-dark cycle was 12:12h.

[0052] Step 2: After the cultivation is over, take out the coriander, wash it with sterile water, and put it in an oven for 48 hours at 80°C. After drying, grind it into powder and sieve it with a 150-mesh sieve .

[0053] Step 3: Weigh 50g of sieved powder into a 1L Erlenmeyer flask, add 400mL of sterile water, place in a shaker, and extract at 28°C for 3 days.

[0054] The fourth step: after the extraction, the extract was centrifuged at 3500r / min for 15min, and then filtered with a glass fiber membrane (GF / F membrane) with a pore size of 25mm to o...

Embodiment 3

[0060] Inhibitory effect of different concentrations of scallop powder-sodium alginate pellets on Microcystis aeruginosa

[0061] Experimental materials: Microcystis aeruginosa was purchased from the Institute of Hydrobiology, Chinese Academy of Sciences. BG11 medium was used to expand the cultivation to logarithmic growth phase under the conditions of light of 2700lx and light-dark ratio of 12:12h.

[0062] Add 150mL of BG11 culture solution into a 250mL Erlenmeyer flask, after high-pressure steam sterilization, insert 10mL of Microcystis aeruginosa liquid with the same growth under sterile conditions, cultivate to the logarithmic phase, and use a hemocytometer under the microscope The cell density of Microcystis aeruginosa was observed, with an average of 5.6×10 5 pcs / mL, add 2 mg, 4 mg, 6 mg, and 8 mg of the coriander-sodium alginate pellets prepared in Example 1, respectively, and then add the sterilized BG11 nutrient solution in sequence, so that the total volume of the c...

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Abstract

The invention discloses a potamogeton malaianus-sodium alginate pellet-type algistat as well as a preparation method and application thereof and belongs to the field of preparation of macromolecule materials. The algistat is prepared by taking submerged plant potamogeton malaianus extract as a raw material, taking sodium alginate as a coating solution and taking calcium chloride as a cross-linking agent. Compared with the prior art, natural submerged plant with allelopathic effect is used as the raw material, the algistat is easily degradable, is high in alga inhibiting efficiency and is capable of effectively overcoming the defects of secondary pollution caused by chemical and physical methods and high possibility of damage to the water ecological environment; potamogeton malaianus extract powder is coated by sodium alginate, so that the algistat is capable of effectively resisting dilution effect of water flow and overcoming the problem of short alga inhibiting duration; and the efficient alga inhibiting effect is achieved.

Description

technical field [0001] The invention belongs to the field of polymer material preparation, and more specifically, relates to an anti-algae-sodium alginate and its preparation method and application. Background technique [0002] As the eutrophication of the lake deepens, the concentration of nitrogen and phosphorus increases, leading to the overgrowth of cyanobacteria in the lake. Cyanobacteria bloom has become an ecological disaster in eutrophic lakes. my country is one of the countries with the most serious outbreaks of cyanobacteria blooms, the most widely distributed and the most species of cyanobacteria blooms in the world. For example, Taihu Lake, Dianchi Lake and Chaohu Lake all have large-scale and high-frequency cyanobacteria blooms, and in some bloom accumulation areas, the concentration of cyanobacteria has exceeded 10 10 cells / L. Cyanobacteria blooms can also cause "secondary pollution" of water bodies, such as odorous compounds or toxins secreted by cyanobact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/40A01N25/10A01P13/00
CPCA01N25/10A01N65/00A01N65/40A01N2300/00
Inventor 张毅敏杨飞马梦洁高月香彭福全孔明
Owner NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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