A kind of method that takes hydroxyproline waste water as raw material to prepare l-alanine

A technology of hydroxyproline and alanine, which is applied in the field of hydroxyproline wastewater reuse, can solve the problems of many types of impurities in L-alanine, complex L-alanine process, and many impurity amino acids, etc. Achieve the effect of sustainable development, good production and operation environment, and simple refining process

Active Publication Date: 2018-04-03
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

In addition, screening from a culture medium of Pseudomonas de aquinha that can effectively produce L-aspartic acid-β-decarboxylase to obtain a high-yield and high-enzyme activity fermentation source for the production of L-alanine , but the process of producing L-alanine by fermentation is complex
Hydroxyproline and proline open the ring under high temperature conditions to produce L-alanine. However, due to the complex composition of gelatin and the incomplete oxidation reaction in the production process of L-hydroxyproline, the waste water There are many impurity amino acids and large pigment content, resulting in the formation of L-alanine with many types of impurities, large amounts, and difficult separation

Method used

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  • A kind of method that takes hydroxyproline waste water as raw material to prepare l-alanine
  • A kind of method that takes hydroxyproline waste water as raw material to prepare l-alanine

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Embodiment 1

[0021] This embodiment relates to a method for preparing L-alanine from hydroxyproline wastewater, which specifically includes the following steps:

[0022] a. Take hydroxyproline waste water, put it in the reactor at a high temperature of 110°C, react for 6 hours, and centrifuge the solution to obtain L-alanine mother liquor;

[0023] b. Decolorize the mother liquor obtained in step a with 3% (mass fraction) food-grade activated carbon added at 65°C for 2 hours, filter to obtain a colorless and transparent solution, and concentrate under reduced pressure to obtain a solid;

[0024] c. Add "methanol+water" to the solid obtained in step b, wherein the mass ratio of methanol:water=90:10, dissolve at 55°C for 2 hours, filter and dry to obtain crude L-alanine;

[0025] d. Recrystallize the crude L-alanine obtained in step c, prepare a saturated aqueous solution of L-alanine at 65°C, fully dissolve it for 2 hours, and add absolute ethanol at a dropping rate of 1 g / min until it is i...

Embodiment 2

[0027] This embodiment relates to a method for preparing L-alanine from hydroxyproline wastewater, which specifically includes the following steps:

[0028] a. Take hydroxyproline waste water, put it in the reactor at a high temperature of 100° C., react for 7 hours, and centrifuge the solution to obtain L-alanine mother liquor.

[0029] b. Decolorize the water obtained in step a with 0.5% (mass fraction) food-grade activated carbon added at 60°C for 3 hours, filter to obtain a colorless and transparent solution, and concentrate under reduced pressure to obtain a solid.

[0030] c. Add "absolute ethanol + water" to the solid obtained in step b, wherein absolute ethanol:water=92:8. Dissolve at 60°C for 3h, filter and dry to obtain crude L-alanine.

[0031] d. Recrystallize the crude L-alanine obtained in step c, prepare a saturated aqueous solution of L-alanine at 70°C, fully dissolve it for 2 hours, and add isopropanol at a dropping rate of 3g / min until it is in the solution ...

Embodiment 3

[0033] This embodiment relates to a method for preparing L-alanine from hydroxyproline wastewater, which specifically includes the following steps:

[0034] a. Take hydroxyproline waste water, put it in the reactor at a high temperature of 80°C, react for 8 hours, and centrifuge the solution to obtain L-alanine mother liquor.

[0035] b. Take the gelatin wastewater, decolorize it with 1% (mass fraction) food-grade activated carbon at 65°C for 1.5 hours, filter to obtain a colorless and transparent solution, and concentrate under reduced pressure to obtain a solid.

[0036] c. Add "n-butanol + water" to the solid obtained in step a, wherein n-butanol:water=95:5. Dissolve at 50°C for 2h, filter and dry to obtain crude L-alanine.

[0037] d. Recrystallize the crude L-alanine obtained in step b, prepare a saturated aqueous solution of L-alanine at 55°C, fully dissolve it for 2 hours, and add isopropanol at a dropping rate of 3g / min until it is in the solution When fine crystals ...

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Abstract

The invention relates to a method for recycling hydroxyproline wastewater, in particular to a method for preparing L-alanine by taking hydroxyproline process wastewater as a raw material. L-alanine is prepared by taking the hydroxyproline wastewater as the raw material through a high-temperature oxidization method and then separated and purified through the coupling processes of activated carbon decoloration and concentration, alcohol solution separation and alcohol (ketone) solution recrystallization. It is determined that the obtained product is a pure L-alanine product through nuclear-magnetic analysis and specific rotation determination. According to the method, wastewater recycling is achieved, the wastewater treatment cost is reduced, and the sustainable development goal of resources and environment is achieved.

Description

technical field [0001] The invention relates to a method for reusing hydroxyproline waste water, in particular to a method for preparing L-alanine by using hydroxyproline production process waste water as a raw material. Background technique [0002] The gelatin hydrolyzate used to produce hydroxyproline contains a large amount of amino acids, including glycine 39.56mg / mL, hydroxyproline 21.65mg / mL, proline 21.71mg / mL, and phenylalanine 15.75mg / mL , arginine 9.8mg / mL and other 20 kinds of amino acids. Gelatin hydrolysis-oxidation method is commonly used in industry to produce hydroxyproline. This production process uses gelatin particles as raw materials, hydrochloric acid hydrolysis, sodium nitrite oxidation, high temperature reduction, activated carbon decolorization and ion exchange processes to obtain hydroxyproline. [0003] Gelatin wastewater is the post-column analysis solution after collecting L-hydroxyproline through ion exchange, which contains a large amount of p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C227/18C07C227/40C07C227/42C07C229/08
CPCC07B2200/07C07C227/18C07C227/40C07C227/42C07C229/08
Inventor 段二红李雪陈子丹王坤坤马焕金俞磊牛建瑞罗晓
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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