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Preparation method and application of bio-fermentation high-efficiency amino acid type organic enzyme preparation

An amino acid type, bio-fermentation technology, applied in the direction of biochemical equipment and methods, applications, glycosylase, etc., can solve the problems of affecting the earth's ecological environment, the quality of agricultural products, and the long-term effect is not optimistic, so as to improve the freshness of crops Ability, strong fresh-keeping force, high-efficiency and long-lasting effect

Active Publication Date: 2020-05-05
海南博士威生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] As we all know, modern agricultural methods use a large amount of chemical fertilizers and pesticides. Although this method can greatly increase crop yields and make full use of the land, it is accompanied by a series of problems: environmental pollution, land rancidity and abandoned farming, increasingly serious pests and diseases, and the decline in the quality of agricultural products. Fertilizer and pesticide residues, etc., and these problems are becoming more and more serious, directly affecting people's lives and the ecological environment of the entire earth
[0003] However, due to cost considerations, the current production process of amino acids mostly uses hydrochloric acid, and rarely sulfuric acid.
Therefore, the stock solution generally contains 12-14% ammonium chloride, and the chloride ion content is high, reaching about 10%. It is easy to cause root burn and seedling burn when used as foliar fertilizer
Although the quick-acting effect of amino acid foliar fertilizer is good, the long-lasting effect is not optimistic

Method used

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  • Preparation method and application of bio-fermentation high-efficiency amino acid type organic enzyme preparation
  • Preparation method and application of bio-fermentation high-efficiency amino acid type organic enzyme preparation
  • Preparation method and application of bio-fermentation high-efficiency amino acid type organic enzyme preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment one: the preparation method of biofermentation efficient amino acid type organic enzyme preparation, the steps are as follows:

[0028] (1) Mix 3 kilograms of kelp, 2 kilograms of leaves, 2 kilograms of hay and 3 kilograms of fish meal in proportion, break them up, add water until the water content is 30%, perform high-pressure sterilization to obtain fermentation raw materials, and pour them into a fermentation tank for later use.

[0029] (2) Preparation of enzyme bacteria liquid: First, put the enzyme bacteria strains in an incubator at 36°C for 12 hours, and then spread the revived bacteria on an agar plate with an inoculation loop, put them in an incubator and continue at 36°C Cultivate at a constant temperature for 36 hours, take 4 Erlenmeyer flasks and fill them with 200ml medium respectively, transfer the cultured flora into the Erlenmeyer flasks, close the mouth of the flasks, put them in a shaker, and culture them anaerobically at a constant temperatur...

Embodiment 2

[0033] Embodiment 2: detection containing enzymes

[0034] Take the bio-fermentation high-efficiency amino acid type organic enzyme preparation obtained in Example 1, dilute it 50 times in a sterile environment, and put 65 μL of the dilution solution into the cups on the API ZYM plate. Adjust the plate temperature to 37C° and incubate for 5 hours. Then, add 1 drop each of ZYM A reagent and ZYM B reagent, and color for 5 minutes. Use a strong light source to eliminate residual Fast Blue (yellow pigment). After light exposure, the plates were left to stand for 2 minutes. Read the result. Results: The enzymes contained in the bio-fermentation high-efficiency amino acid type organic enzyme preparation are as follows: amidase 7.7mmol / L, alkaline phosphatase 7.7mmol / L, esterase (C4) 7.7mmol / L, esterase lipase (C8) 7.7mmol / L, valine aryl aminopeptidase 3.8mmol / L, acid phosphatase 3.8mmol / L, naphthol AS-BI phosphohydrolase 7.7mmol / L, β-galactosidase 3.8mmol / L, β-glucuronidase 5nm...

Embodiment 3

[0035] Embodiment three: the preparation of contrast amino acid organic enzyme preparation A

[0036] (1) Mix 3 kilograms of kelp, 2 kilograms of leaves, 2 kilograms of hay and 3 kilograms of fish meal in proportion, break them up, add water until the water content is 30%, perform high-pressure sterilization to obtain fermentation raw materials, and pour them into a fermentation tank for later use.

[0037] (2) Preparation of enzyme bacteria liquid: First, put the enzyme bacteria strains in an incubator at 36°C for 12 hours, and then spread the revived bacteria on an agar plate with an inoculation loop, put them in an incubator and continue at 36°C Cultivate at a constant temperature for 36 hours, take 4 Erlenmeyer flasks and fill them with 200ml medium respectively, transfer the cultured flora into the Erlenmeyer flasks, close the mouth of the flasks, put them in a shaker, and culture them anaerobically at a constant temperature of 36°C for 8 hours to obtain a culture medium, ...

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Abstract

The invention discloses a preparation method and applications of a biologically-fermented high-efficiency amino-acid-type organic enzyme preparation. The biologically-fermented high-efficiency amino-acid-type organic enzyme preparation is prepared via two times of biological fermentation of raw materials including kelp, plant raw materials, and fish meal. According to the preparation method, two times of fermentation is adopted, so that full utilization of the raw materials is realized, fermentation products are abundant, and contain main medium elements, micro elements, a plurality of amino acids, and enzymes which are necessary for plants. The biologically-fermented high-efficiency amino-acid-type organic enzyme preparation possesses high efficiency and obvious yield increasing effect on crops such as wheat, rice, cotton, soybean, peanut, oilseed rape, sugarbeet, and a plurality of vegetables and fruit trees, is green, is safe for crops, and is high in efficiency, the effect lasts long, no pollution is caused, and application cost is low.

Description

technical field [0001] The invention relates to a bio-fermentation high-efficiency amino acid type organic enzyme preparation, in particular to a preparation method and application of a bio-fermentation high-efficiency amino acid type organic enzyme preparation using kelp, plant raw materials and fish meal as raw materials. Background technique [0002] As we all know, modern agricultural methods use a lot of chemical fertilizers and pesticides. Although this method can greatly increase crop yields and make full use of the land, it is accompanied by a series of problems: environmental pollution, land rancidity and abandoned farming, increasingly serious pests and diseases, and the decline in the quality of agricultural products. Fertilizer and pesticide residues, etc., and these problems are becoming more and more serious, directly affecting people's lives and the ecological environment of the entire earth. Amino acid foliar fertilizer is a foliar fertilizer containing amino...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/78C12N9/16C12N9/20C12N9/48C12N9/14C12N9/38C12N9/24C12N9/42A23B7/155C05G3/00
CPCA23B7/155C05F1/002C05G3/00C05G5/20C12N9/14C12N9/16C12N9/20C12N9/2445C12N9/2471C12N9/485C12N9/78C12Y301/03001C12Y301/03002C12Y302/01021C12Y302/01023C12Y305/99002C05F11/00C05F11/08
Inventor 吴淑科
Owner 海南博士威生物科技有限公司
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