Proline aminopeptidase detection reagent, reaction pad, preparation method and kit thereof

A proline aminopeptidase and detection reagent technology, which is applied in biochemical equipment and methods, biological testing, measuring devices, etc., can solve problems such as lack of accuracy, and achieve the effects of good accuracy, high sensitivity, and accurate detection

Active Publication Date: 2018-06-08
GUANGZHOU HONGQI OPTICAL INSTR TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection reagents currently used to detect proline aminopeptidase are not accurate enough

Method used

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  • Proline aminopeptidase detection reagent, reaction pad, preparation method and kit thereof
  • Proline aminopeptidase detection reagent, reaction pad, preparation method and kit thereof
  • Proline aminopeptidase detection reagent, reaction pad, preparation method and kit thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0044] The preparation method of the reaction pad includes the following steps:

[0045] 1) Material preparation: prepare dithiothreitol, sucrose, polyvinylpyrrolidone, Z-glycyl-proline-p-nitroaniline and bovine serum albumin according to the above weight parts;

[0046] 2) Prepare buffer: prepare 0.01mol / L~1mol / L Tris-HCl buffer;

[0047] 3) preparing soaking solution: dissolving dithiothreitol, sucrose, polyvinylpyrrolidone, Z-glycyl-proline-p-nitroaniline and bovine serum albumin in Tris-HCl buffer to obtain soaking solution;

[0048] 4) soaking solution treatment: the filter paper is fully soaked in the soaking solution for 10-20s, then taken out, and then dried at a temperature of 20-75°C and a time of 30-40min to obtain a reaction pad.

[0049] More specifically, the filter paper can be Whatman glass fiber filter paper, GF / A Whatman's fiber filter paper Grade 3 or ordinary quantitative filter paper.

[0050] Use the reaction pad to prepare the kit: place the reaction p...

Embodiment 1

[0052] Examples 1 to 3 disclose proline aminopeptidase detection reagents. Three groups of raw material components are prepared according to Table 1, and Tris-HCl buffers with different concentrations are prepared according to Table 2; group A is used in Example 1, and group B is used. For Example 2, Group C was used for Example 3.

[0053] Dithiothreitol, sucrose, polyvinylpyrrolidone, Z-glycyl-proline-p-nitroaniline and bovine serum albumin were dissolved in 100 mL of Tris-HCl buffer in turn to obtain proline aminopeptidase detection reagents.

[0054] Table 1 Composition and content of detection reagents

[0055]

[0056] Table 2 Preparation parameters of Tris-HCl buffer

[0057]

[0058]Detect the detection reagents obtained in Examples 1 to 3: prepare proline aminopeptidase standard solution with different enzyme activity concentrations respectively, the proline aminopeptidase standard solution uses physiological saline as solvent, and detect Example 1 ~3 sensiti...

Embodiment 4～6

[0065] Embodiment 4~6 discloses a kind of reaction pad and preparation method thereof:

[0066] 1) Prepare materials according to Table 1, prepare Tris-Hcl buffer solution according to Table 2; Group A is used in Example 4, Group B is used in Example 5, and Group C is used in Example 6;

[0067] 2) Prepare soaking solution: dissolve dithiothreitol, sucrose, polyvinylpyrrolidone, Z-glycyl-proline-p-nitroaniline and bovine serum albumin in Tris-Hcl buffer to obtain soaking solution;

[0068] 3) Soaking solution treatment: put the filter paper in the soaking solution and fully soak it for 10-20 seconds, then take it out, and then dry it at a temperature of 45° C. for 40 minutes to obtain a reaction pad.

[0069] Detect the reaction pads obtained in Examples 4 to 6: prepare standard proline aminopeptidase solutions with different enzyme activity concentrations respectively, and use physiological saline as a solvent for the proline aminopeptidase standard solution, and test Example...

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Abstract

The invention discloses a proline aminopeptidase detection reagent, a reaction pad, a preparation method of the reaction pad, and a kit; the proline aminopeptidase detection reagent is prepared from dithiothreitol, sucrose, polyvinylpyrrolidone, Z-glycyl-proline-paranitroaniline and bovine serum protein; the preparation method of the reaction pad comprises the steps of 1) preparing a soaking liquid, to be specific, dissolving dithiothreitol, sucrose, polyvinylpyrrolidone, Z-glycyl-proline-paranitroaniline and bovine serum protein in Tris-Hcl buffer solution to obtain the soaking liquid; 2) treating with the soaking liquid, to be specific, sufficiently soaking a carrier in the soaking liquid, removing, and drying to obtain the reaction pad; the high-sensitivity high-precision reaction pad is acquired by using the preparation method in connection with the components of the detection reagent.

Description

technical field [0001] The invention relates to the field of bacterial vaginitis detection reagents, in particular to a proline aminopeptidase detection reagent, a reaction pad and a kit. Background technique [0002] Vaginitis is inflammation of the vaginal mucosa and submucosal connective tissue, and is a common disease in gynecological outpatient clinics. In normal healthy women, due to anatomical and biochemical characteristics, the vagina has a natural defense function against the invasion of pathogens. When the natural defense function of the vagina is destroyed, pathogens are easy to invade, resulting in vaginal inflammation. Common vaginitis is bacterial vaginitis (BV / AV), fungal vaginitis (VVC) and trichomonas vaginitis (TV). Among them, female bacterial vaginal infection can be divided into two types, one is caused by anaerobic bacteria and facultative anaerobic bacteria, which is clinically called bacterial vaginosis (BV), and the other is caused by aerobic bacte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/34
CPCG01N33/531G01N33/543G01N33/56911G01N33/56922G01N33/573G01N33/68G01N2800/36
Inventor 眭红燕朱华琳范静彦李必松
Owner GUANGZHOU HONGQI OPTICAL INSTR TECH
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