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Quality detection method for dysuria remedying granules

A kind of closed and granule technology, which is applied in the field of analysis of traditional Chinese medicine preparations, can solve the problems of lower life happiness index, huge economic burden, and unsatisfactory treatment effect, etc.

Active Publication Date: 2017-01-11
LUNAN HOPE PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chronic prostatitis does not endanger the patient's life, it seriously affects the patient's physical and mental health and quality of life, and reduces the happiness index of life. In particular, the high cost of treatment brings a huge economic burden to the patient's family
[0003] Modern medicine has not fully elucidated the etiology and pathological changes of this disease, and there is also a lack of effective methods for treatment. It still relies on antibiotics, physical therapy, etc., and the treatment effect is not very satisfactory.

Method used

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  • Quality detection method for dysuria remedying granules
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  • Quality detection method for dysuria remedying granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The HPLC methodology research of embodiment 1 berberine hydrochloride

[0066] 1) Instruments and reagents: Agilent 1100 high performance liquid chromatography, VWD detector. Acetonitrile was chromatographically pure, other reagents were analytically pure, and water was double distilled water.

[0067] 2) Chromatographic conditions: Kromasil C 18 Chromatographic column (4.6mm×250mm, 5μm); mobile phase is acetonitrile: 0.05mol / L sodium dihydrogen phosphate (containing 0.5% triethylamine, adjust pH value to 3.0 with phosphoric acid) = 30:70; detection wavelength is 346nm; flow rate 1mL / min. The number of theoretical plates should not be less than 3000 based on the berberine hydrochloride peak.

[0068]3) Preparation of the test solution: take the product under the difference in loading amount, grind it finely, take 2g, accurately weigh it, put it in a stoppered Erlenmeyer flask, accurately add 50mL of hydrochloric acid:methanol=1:100 solution, weigh Determine the weig...

Embodiment 2

[0087] Embodiment 2 Amygdalin HPLC methodological research

[0088] 1) Instruments and reagents: Agilent 1100 high performance liquid chromatography, VWD detector. Methanol is chromatographically pure, other reagents are analytically pure, and water is double distilled water.

[0089] 2) Chromatographic conditions: Kromasil C 18 Chromatography column (4.6mm×250mm, 5μm); mobile phase is methanol:water=18:82; detection wavelength is 210nm; flow rate is 0.8mL / min. The number of theoretical plates should not be less than 3000 based on the peak of amygdalin.

[0090] 3) Preparation of the test solution: take the product under the item of difference in loading amount, grind it finely, take 1g, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 50mL of water precisely, weigh it, and ultrasonically treat it for 30 minutes , let it cool, weigh again, make up the lost weight with water, shake well, centrifuge, accurately measure 25mL of the supernatant, put it in a sepa...

Embodiment 3

[0108] Example 3 TLC Identification of Cinnamon in Longbuxintong Granules

[0109] 1) Preparation of the test solution: Take 10g of this product, grind it finely, add 50mL of methanol, ultrasonically extract for 30 minutes, shake occasionally, filter, evaporate the filtrate to dryness, add 20mL of water to the residue to dissolve, adjust the pH value with dilute hydrochloric acid To 1-2, extract with diethyl ether twice, 20 mL each time, combine the diethyl ether solution, evaporate to dryness at low temperature, add 2 mL of methanol to the residue to dissolve, and use it as the test solution;

[0110] 2) Preparation of the reference substance solution: take the cinnamon aldehyde reference substance and add ethanol to make a solution containing 1 μL per 1 mL, as the reference substance solution;

[0111] 3) Spotting and development: Take 2-5 μL of the test solution and 2 μL of the reference solution, and place them on the same silica gel G thin-layer plate respectively, using ...

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Abstract

The invention discloses a quality detection method for Chinese patent medicine dysuria remedying granules for treating chronic prostatitis. The quality detection method comprises the steps that the thin layer chromatography is adopted for recognizing cinnamon, Chinese violets, radix scrophulariae, golden cypress, radix sophorae flavescentis, rhizoma dioscoreae hypoglaucae and radix cyathulae in the preparation, and meanwhile HPLC is adopted for determining the content of berberine hydrochloride of the golden cypress in the preparation and the content of amygdalin in peach kernels. The quality detection method is stable, reliable, high in specificity, good in reproducibility, capable of comprehensively and effectively controlling the quality of the dysuria remedying granules, and capable of stabilizing the product quality, ensuring the clinical medication safety and effectiveness and better meeting the requirement for medical treatment and market.

Description

technical field [0001] The invention relates to a quality detection method of Longguanxintong granule, a Chinese patent medicine for treating chronic prostatitis, and belongs to the field of analysis of traditional Chinese medicine preparations. Background technique [0002] Chronic prostatitis (CP) is a common and intractable disease in urology and andrology, and it often occurs in young and middle-aged men. Its etiology and pathogenesis are complex, characterized by a long course of disease, persistent recurrence, and various symptoms. The main clinical manifestations are abnormal urination, pain or discomfort in the pelvic region, or even accompanied by sexual dysfunction. Although chronic prostatitis does not endanger the patient's life, it seriously affects the patient's physical and mental health and quality of life, reduces the life happiness index, and especially the high cost of treatment brings a huge economic burden to the patient's family. [0003] Modern medici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/90
CPCG01N30/02G01N30/90
Inventor 张则平张贵民刘武占庄会芳
Owner LUNAN HOPE PHARM CO LTD
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