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Stable uric acid reagent with high anti-interference capacity and detection method

A detection method and technology of reagents, which are applied in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problems of complex sample pretreatment, interference of reducing substances, and expensive equipment. , to achieve the effect of enhancing stability and anti-interference ability, improving stability, accuracy and stability

Inactive Publication Date: 2017-01-04
章丘美高义医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is simple to operate, but it cannot be tested when there are hemolyzed samples, and it is easily interfered by other reducing substances.
Although the HPLC detection method is simple, the sample pretreatment is more complicated, and the equipment is expensive and difficult to popularize
The most popular method now is the uricase-peroxidase coupling method, which is sensitive and does not require protein removal, but is easily affected by interfering substances such as vitamin C and bilirubin

Method used

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  • Stable uric acid reagent with high anti-interference capacity and detection method
  • Stable uric acid reagent with high anti-interference capacity and detection method
  • Stable uric acid reagent with high anti-interference capacity and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The detection reagent of uric acid comprises reagent R1 and reagent R2.

[0045] The composition of its R1 is:

[0046] PIPES (piperazine-1,4-diethanesulfonic acid) buffer (pH=7.6, 25°C) ···························· 100mmol / L

[0047] 4-Aminoantipyrine ···························································································· 1.3mmol / L

[0048] BSA ··············································································································· 1g / L

[0049] sucrose ··············································································································· 5g / L

[0050] Trehalose ··········································································································· 2g / L

[0051] peroxidase ··································································································· 12KU / L

[0052] ascorbate oxidase ···································································...

Embodiment 2

[0071] Interference test: take fresh mixed serum, divide it into 2 equal parts, then divide each equal part into 5 equal parts, add different interfering substances, so that the concentration in the serum reaches the requirements in Table 2. Then, the reagents obtained in Example 1 were used to compare with the common and recognized uric acid (UA) reagents in the market to measure the content of UA in serum at the same time. Relative deviation (%) = (measuring mean value of interference samples - measuring mean value of control samples) / measured mean value of control samples × 100%.

[0072] It can be seen from Table 2 that the reagent of Example 1 has no obvious interference on the test results when ascorbic acid ≤ 1704 μmol / L, bilirubin ≤ 684 μmol / L, hemoglobin ≤ 10 g / L, and triglyceride ≤ 22.6 mmol / L. However, the reagents of the control group were significantly interfered in the presence of the above-mentioned concentration of interfering substances, which shows that the a...

Embodiment 3

[0076] Correlation experiment: using the formula in Example 1 to prepare reagents, and conducting a control test with a uric acid kit from a company approved by the State Food and Drug Administration, which is common in the market, and testing 20 clinical serum samples at the same time, the test results are shown in Table 3 . And obtained the correlation curve of the two reagents (such as figure 1 Shown), the test results show that the correlation coefficient of the two kits is 0.9992, indicating that there is a great correlation between the two.

[0077] surface 3 Example 1 The test results are compared with the common and recognized uric acid assay kits in the market

[0078] 。

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Abstract

The invention relates to the technical field of uric acid detection, in particular to a uric acid detection reagent. A reagent R1 is prepared from a buffer solution, 4-aminoantipyrine, BSA, sucrose, trehalose, peroxidase, ascorbic acid oxidase, bilirubin oxidase, alkylphenol ethoxylates (APEO) and a preservative; a reagent R2 is prepared from a buffer solution, TOOS uricase.BSA sucrose.trehalose alkylphenol ethoxylates (APEO) and a preservative. The PIPES (piperazine-1,4-bisethanesulfonic acid) solution is adopted, the stabilizer BSA, sucrose and trehalose are added, and the stability of the reagent is greatly improved; the novel surfactant alkylphenol ethoxylates (APEO) is adopted, and therefore not only is the determination property of the product significantly improved, but also the stability and anti-interference capacity of the reagent are improved.

Description

technical field [0001] The invention relates to the technical field of uric acid detection, in particular to a uric acid detection reagent and a detection method using the detection reagent. Background technique [0002] Uric acid is the end product of purine catabolism. Excreted by the kidneys with urine. The content of uric acid in healthy adults is about 1.1g, of which about 15% exists in the blood. After the uric acid in the blood is filtered by the glomeruli, most of it is reabsorbed by the renal tubules. Uric acid is one of the important components of non-protein nitrogen in plasma. In severe kidney damage, uric acid in blood can increase significantly, but it does not change much in mild damage. Therefore, the determination of serum uric acid is a sensitive indicator for the diagnosis of severe renal damage. [0003] At present, the detection methods of uric acid (UA) include phosphotungstic acid reduction method, uricase method and HPLC method. The tungstic acid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 谭伯清李志明甘宜梧肖慧王绮
Owner 章丘美高义医疗器械有限公司
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