Paper chip for detecting residual antibiotic in milk

Abstract

The invention relates to a paper chip for detecting the residual antibiotic in milk, particularly to a paper chip detection method for the residues of five antibiotics such as tetracycline, chloramphenicol, sulfanilamide, quinolone and beta-lactam in milk. A purpose of the present invention is to provide a multi-channel detection paper chip. According to the present invention, the detection quantity, the detection index and the detection time are improved, the opening platform is provided, and the detection index on the paper chip can be adjusted according to the actual demand; and the results show that the paper chip has advantages of low cost, high sensitivity, platform opening, accurate result and the like, and provides a certain guidance significance for the development of the paper chip in the food safety detection field.

Description

technical field [0001] The invention relates to the field of rapid detection of food safety, in particular to the field of rapid detection of antibiotic residues in milk. Background technique [0002] The widespread use of antibiotics in animal husbandry inevitably results in antibiotic residues in milk. Antibiotic residues not only endanger human health, but also affect the quality of milk and cause economic losses. In the past, there were no explicit regulations on the sanitary indicators of antibiotic residues in milk in my country. Until October 2001, the Ministry of Agriculture issued and implemented the "Industry Standards for Pollution-Free Food Raw Milk", which clarified the sanitary indicators of fresh milk that "antibiotics must not be detected". However, this industry standard has not been strictly implemented in our country at present, resulting in the problem of antibiotic residues in milk and dairy products in our country has not been resolved, which has arous...

AI Technical Summary

Problems solved by technology

However, this industry standard has not been strictly implemented in our country at present, resulting in the problem of antibiotic residues in milk and dairy products in our country has not been resolved, which has aroused people's concerns about the safety of milk and has become an issue of increasing concern. hotspot

[0003] At present, the main detection methods used are: microbial detection method, the principle of which is to qualitatively or quantitatively determine the antimicrobial drug residues in the sample according to the inhibitory effect of antibiotics on the physiological function and metabolism of microorganisms, such as the disk method (PD), TTC method, swab method (STOP), etc.; physical and chemical detection method, physical and chemical detection method is to use the special reaction or property of the group in the antibiotic molecule to determine its content, such as high performance liquid chromatography, gas chromatography, mass spectrometry method, combined technology, etc., can carry out qualitative, quantitative and drug identification, with high sensitivity, but some detection procedures are more complicated, and some detection costs are higher; immune method, the current drug residue immunoassay technology is mainly divided into two types: Major categories: one is a relatively independent analysis method, that is, immunoassays (IAs), such as RIA, ELISA, solid phase immunosensor (soindphase immunosensor), etc.; the other is the combination of immunoassay technology and conventional physical and chemical analysis technology, For example, the high selectivity of immunoassay is used as a purification method in physical and chemical determination technology, and the typical method is immunoaffinity chromatography (IAC)

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