Microbial preparation for rapid treatment of soil with heavy metal pollution and production method thereof

A technology for microbial preparations and polluted soil, which is applied in the field of microbial preparations for rapid treatment of heavy metal-contaminated soils. It can solve problems such as high economic cost, poor universality, and incompleteness, and achieve high bacterial mortality, improve soil fertility, and adsorption capacity. strong effect

Inactive Publication Date: 2016-12-14
宁波枫叶杰科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Soil remediation appears more difficult than air and water pollution
Su Dechun, a member of the Ministry of Agriculture's expert group on the prevention and control of heavy metal pollution in agricultural product areas and a professor at the School of Resources and Environment of China Agricultural University, said that not only is soil pollution a serious hazard, but it is also different from some organic pollutants in that heavy metals in soil cannot be degraded, and it is very difficult to separate them from the soil. , it is very difficult to repair
[0004] Among the relevant patents that have been applied in China, the patent "A Microbial Remediation Method for Heavy Metal Contaminated Soil" (Application No.: 201110122739.6, Publication Date: 2011-11-16) discloses a method of soil treatment using Rhodobacter sphaericus preparations combined with flooding The method, whose working principle is mainly to use Rhodobacter sphaericus to absorb heavy metals after multiplying in a large amount in a water-rich anaerobic environment, and then infiltr...

Method used

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Embodiment 1

[0013] A microbial preparation for rapidly treating heavy metal-contaminated soil, wherein: the microbial preparation is composed of the following components by weight: 25-40 parts of Aspergillus niger inoculum, 25-40 parts of Rhizopus niger inoculum, and 10 parts of brewer's yeast inoculum -15 parts, 5-10 parts of filamentous cyanobacteria preparations, 15-20 parts of Listeria monocytogenes inoculum; the Aspergillus niger inoculum is made by implanting purified Aspergillus niger in the GSH protection solution, The concentration of Aspergillus niger is 50×10 8 cfu / ml-100×10 8 cfu / ml, said Rhizopus niger inoculum is made by implanting the purified Rhizopus niger in the GSH protection solution, and the concentration of Rhizopus niger is 20×10 8 cfu / ml-50×10 8 cfu / ml; the brewer's yeast inoculant is prepared by implanting purified brewer's yeast in distilled water, and the concentration of brewer's yeast is 30×10 8 cfu / ml-80×10 8 cfu / ml, the bacterial agent is stored in an en...

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Abstract

The invention discloses a microbial preparation for rapid treatment of soil with heavy metal pollution and a production method thereof. The microbial preparation comprises the following components in parts by weight: 25-40 parts of an Aspergillus niger agent, 25-40 parts of a Rhizopus nigricans agent, 10-15 parts of a Saccharomyces cerevisiae agent, 5-10 parts of a filamentous cyanobacteria agent, and 15-20 parts of a Listeria monocytogenes agent; the Aspergillus niger agent is preserved in GSH protective solution; the Saccharomyces cerevisiae agent is preserved in distilled water; the filamentous cyanobacteria agent is preserved in a glycerin solution whose mass percent is 30%; the Listeria monocytogenes agent is preserved in a glycerin solution whose mass percent is 10%. The invention also discloses a purification, cultivation, preservation and production method of the microbial preparation. The microbial preparation has the advantages of multiple bacterial strains which effective complemented, high efficiency, good economical efficiency, and wide application range.

Description

technical field [0001] The invention relates to the field of heavy metal prevention and control, in particular to a microbial preparation for rapidly treating heavy metal-contaminated soil and a preparation method thereof. Background technique [0002] Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds. It is mainly caused by human factors such as mining, exhaust gas discharge, sewage irrigation and the use of products with heavy metals exceeding the standard. Due to human activities, the content of heavy metals in the environment increases, which exceeds the normal range, directly endangers human health, and leads to the deterioration of environmental quality. At the beginning of April 2011, my country's first "12th Five-Year" special plan - "Heavy Metal Pollution Comprehensive Prevention and Control "12th Five-Year Plan" was officially approved by the State Council, and the prevention and control plan strives to control 5 kin...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/14B09C1/10C12R1/685C12R1/845C12R1/865C12R1/01
CPCC12N1/20B09C1/105C12N1/14C12N1/18
Inventor 胡奕清
Owner 宁波枫叶杰科生物技术有限公司
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