Microbial preparation for rapid treatment of soil with heavy metal pollution and production method thereof

Abstract

The invention discloses a microbial preparation for rapid treatment of soil with heavy metal pollution and a production method thereof. The microbial preparation comprises the following components in parts by weight: 25-40 parts of an Aspergillus niger agent, 25-40 parts of a Rhizopus nigricans agent, 10-15 parts of a Saccharomyces cerevisiae agent, 5-10 parts of a filamentous cyanobacteria agent, and 15-20 parts of a Listeria monocytogenes agent; the Aspergillus niger agent is preserved in GSH protective solution; the Saccharomyces cerevisiae agent is preserved in distilled water; the filamentous cyanobacteria agent is preserved in a glycerin solution whose mass percent is 30%; the Listeria monocytogenes agent is preserved in a glycerin solution whose mass percent is 10%. The invention also discloses a purification, cultivation, preservation and production method of the microbial preparation. The microbial preparation has the advantages of multiple bacterial strains which effective complemented, high efficiency, good economical efficiency, and wide application range.

Description

technical field [0001] The invention relates to the field of heavy metal prevention and control, in particular to a microbial preparation for rapidly treating heavy metal-contaminated soil and a preparation method thereof. Background technique [0002] Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds. It is mainly caused by human factors such as mining, exhaust gas discharge, sewage irrigation and the use of products with heavy metals exceeding the standard. Due to human activities, the content of heavy metals in the environment increases, which exceeds the normal range, directly endangers human health, and leads to the deterioration of environmental quality. At the beginning of April 2011, my country's first "12th Five-Year" special plan - "Heavy Metal Pollution Comprehensive Prevention and Control "12th Five-Year Plan" was officially approved by the State Council, and the prevention and control plan strives to control 5 kin...

AI Technical Summary

Problems solved by technology

[0003] Soil remediation appears more difficult than air and water pollution

Su Dechun, a member of the Ministry of Agriculture's expert group on the prevention and control of heavy metal pollution in agricultural product areas and a professor at the School of Resources and Environment of China Agricultural University, said that not only is soil pollution a serious hazard, but it is also different from some organic pollutants in that heavy metals in soil cannot be degraded, and it is very difficult to separate them from the soil. , it is very difficult to repair

[0004] Among the relevant patents that have been applied in China, the patent "A Microbial Remediation Method for Heavy Metal Contaminated Soil" (Application No.: 201110122739.6, Publication Date: 2011-11-16) discloses a method of soil treatment using Rhodobacter sphaericus preparations combined with flooding The method, whose working principle is mainly to use Rhodobacter sphaericus to absorb heavy metals after multiplying in a large amount in a water-rich anaerobic environment, and then infiltrate into the ground through water to reduce the concentration of heavy metals in the soil, but the soil cannot be used for planting operations during the treatment process. The economic cost is high. On the other hand, the strains are too single, the universality is poor, the scope of application is small, and the types and degrees of heavy metals that can be treated are low; Publication date: 2015-04-22) discloses a heavy metal-contaminated soil treatment agent using Bacillus subtilis and Candida tropicalis as active ingredients. The bioremediation agent of the invention can absorb a certain proportion of heavy metals and can However, Candida tropicalis is an anaerobic fungus and cannot survive for a long time in dry and ventilated soil, while Bacillus subtilis, as an aerobic bacteria, cannot survive for a long time in wet soil. The two effective species live in the same environment. On the other hand, there are too few effective strains. Since each microorganism has its own limitations, its effect on soil treatment is neither long-term nor comprehensive.