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Sandwich-structure Raman signal enhancement probe for fingerprint imaging and preparation method thereof

A sandwich structure, Raman signal technology, applied in biochemical equipment and methods, Raman scattering, instruments, etc., to achieve the effect of improving universality, improving fingerprint recognition efficiency, and mild binding conditions

Inactive Publication Date: 2016-11-09
SHANGHAI OCEAN UNIV +5
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Combining the advantages of SERS imaging technology and aptamer recognition technology, the development and preparation of Raman signal-enhanced probes suitable for high-definition fingerprint imaging, which are easy to prepare, easy to operate, and stable in signal has become a research hotspot in this field in recent years. The existing lysozyme is used as a target molecule for SERS imaging recognition, so far, there has been no relevant report

Method used

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  • Sandwich-structure Raman signal enhancement probe for fingerprint imaging and preparation method thereof
  • Sandwich-structure Raman signal enhancement probe for fingerprint imaging and preparation method thereof
  • Sandwich-structure Raman signal enhancement probe for fingerprint imaging and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of Sandwich Raman Signal Enhancement Probe for Fingerprint Imaging

[0036] (1) Add 613 μL of aqueous chloroauric acid tetrahydrate (1% (wt / wt)) into a round-bottomed flask containing 50 mL of deionized water, heat to reflux, and add 0.5 mL of trisodium citrate aqueous solution (1 % (wt / wt)) for 20 minutes, stop heating and cool to room temperature to obtain nano-gold sol.

[0037] (2) Add 300 μL ethanol solution (1 mM) of p-nitrothiophenol (pNTP) to the nano-gold sol prepared in step (1), and stir for 4 h to form an Au@pNTP nanoparticle solution.

[0038] (3) 600 μL of 3-aminopropyltrimethoxysilane (1 mM) was added to the Au@pNTP nanoparticle solution prepared in step (2), and the reaction was stirred at room temperature for 30 min. Add 4.8 mL of sodium silicate aqueous solution (0.54% (wt / wt)), and stir the reaction in an oil bath at 90° C. for 30 minutes, then quickly move the round-bottomed flask to an ice-water bath to terminate the reaction. After co...

Embodiment 2

[0043] High-definition SERS imaging of grease-type fingerprints with a sandwich-structured Raman signal-enhanced probe

[0044] (1) Collection of grease-type fingerprints

[0045] Before fingerprint collection, the volunteers washed and dried their hands with soap, wiped their foreheads or nose wings with their fingers, and then gently pressed the fingerprints on the surface of the cleaned glass substrate. To clean the glass substrate, the substrate is washed with soapy water, ethanol, and deionized in sequence.

[0046] (2) High-definition SERS imaging of grease-type fingerprints:

[0047] After aging the collected fingerprints for 16 hours, incubate 200 μL of the sandwich-structured Raman signal enhancement probe prepared in Example 1 at room temperature (25°C-27°C) and a humid (relative humidity of 70%-80%) environment for 30 minutes . Then rinse with deionized water to remove non-specifically adsorbed probes. After drying, use a Horiba XploRA confocal Raman spectrometer...

Embodiment 3

[0050] Sandwich-structured Raman signal-enhanced probe for high-definition SERS imaging of sweat-type fingerprints

[0051] (1) Collection of sweat-type fingerprints

[0052] Before fingerprint collection, the volunteers washed their hands with soap and dried them, put on PE gloves to cover their sweat for 10 minutes, and then pressed gently on the surface of a clean glass substrate for 20 seconds. and deionized cleaning.

[0053] (2) High-definition SERS imaging of sweat-type fingerprints, the steps are the same as step (2) in the above-mentioned embodiment 2.

[0054] For SERS imaging results see image 3 .

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Abstract

The invention relates to a sandwich-structure Raman signal enhancement probe for fingerprint imaging and a preparation method thereof. The probe uses gold nanoparticles as a core, silica as a shell, signal molecules are sandwiched between the core and the shell, and the surface of the shell is modified by using lysozyme ligands for fingerprint identification. The preparation method of the probe includes the steps: (1) preparing the gold nanoparticles; (2) connecting the signal molecules to the gold nanoparticles by S-Au bonds; (3) hydrolyzing sodium silicate so as to perform coating with a silicon layer; and (4) allowing triazinyl to form on the surface of the silicon layer so that the surface has the amino modified lysozyme ligand function. The sandwich-structure probe provided by the invention has the characteristics of good Raman signal enhancement effects, high signal stability, and high fingerprint identification, and the preparation method is simple, and controllable in process, and the prepared Raman signal enhancement probe can be used for high-definition imaging of latent fingerprints.

Description

technical field [0001] The invention belongs to the technical field of functional materials, and in particular relates to a Raman signal enhancement probe with a sandwich structure for fingerprint imaging and a preparation method thereof. Background technique [0002] Fingerprints are the skin texture on the pulp of the end of a human finger, consisting of a series of raised 'ridge lines' and concave 'valley lines' distributed at intervals. Fingerprints vary from person to person and from finger to finger, and are unique; and once a fingerprint is formed, its characteristics remain unchanged for life and are relatively stable. Therefore, fingerprint identification has been widely used in criminal detection and personal identification. Fingerprint identification is carried out by comparing the feature points of different fingerprint details. Generally speaking, the characteristics of fingerprints are divided into three levels, namely pattern (pattern), details (minutia poin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/115G01N21/65
CPCC12N15/115C12N2310/16G01N21/658G01N2021/656
Inventor 赵静静张炜佳刘宝红乔亮金虎林张昆
Owner SHANGHAI OCEAN UNIV
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