Process for extracting pachyman from poria coccus wolf

Abstract

The invention discloses a process for extracting pachyman from poria coccus wolf. The process comprises the following steps: (1) selecting a poria coccus wolf raw material, and conducting ultrafine grinding to obtain poria coccus wolf coarse powder; (2) adding water into the poria coccus wolf coarse powder for soaking, and placing the soaked poria coccus wolf coarse powder in 40000 Hz ultrasonic waves to be treated for 20-25 min; (3) conducting water bathing and then conducting centrifugal separation; (4) conducting enzymolysis separation by adopting a compound enzyme to obtain a filtrate; (5) conducting protein precipitation and filtration, and centrifugally collecting a supernate; (6) concentrating a pachyman extract to be 10 to 20% of the original volume to obtain a pachyman concentrate, and conducting spray drying to obtain coarse pachyman; (7) conducting ultrafiltration, then conducting column chromatography to obtain purified pachyman, and conducting freeze drying so as to obtain the pachyman. According to the process, an ultrasonic-assisted extraction method is utilized, the process steps are simple, the pachyman leaching rate can be obviously increased, the extracted pachyman is low in possibility of stereostructure damage and high in biological activity, and the advantages that the leaching time is shortened, the extraction conditions are mild, and the reaction product is free of toxic or side effect are achieved.

Description

technical field [0001] The invention relates to the technical field of poria cocos processing, in particular to a process for extracting polysaccharides from poria cocos. Background technique [0002] Poria coccus wolf is the dried sclerotia of Polyporaceae fungus Poria cocos. It is a commonly used traditional Chinese medicine, which has the functions of dispersing dampness, invigorating the spleen, and diuresis. Poria cocos polysaccharide (pachyman) has the functions of inhibiting tumor growth and regulating the body's immunity. In recent years, the extraction of Poria cocos polysaccharide generally uses hot water leaching method, alkaline solution extraction method, acid solution leaching method, etc. to first obtain the crude product of Poria cocos polysaccharide, and then obtain the fine product of Poria cocos polysaccharide through a purification process. The hot water extraction method takes a long time and the polysaccharide extraction rate is low, while the acid-bas...

AI Technical Summary

Problems solved by technology

The hot water extraction method takes a long time and the extraction rate of polysaccharide is low, while the extraction procedure of the acid-base solution extraction method is cumbersome and the extraction conditions are severe, which easily destroys the three-dimensional structure of the polysaccharide and limits its biological activity.

In the above traditional extraction methods, the extraction time is longer and the process conditions are difficult to control, which makes the three-dimensional structure of Poria cocos polysaccharide easily destroyed, the biological activity is low, and the extraction effect is not good.