Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation of photo-induced chemical paper chip for detecting expression of polysaccharides on surfaces of cancer cells

A photoelectrochemical, cell surface technology, used in measurement devices, scientific instruments, material analysis by electromagnetic means, etc., can solve the problems of expensive instruments, high biological damage, complicated operations, etc., and achieve low cost and high detection sensitivity. , the effect of simple operation

Inactive Publication Date: 2016-10-12
UNIV OF JINAN
View PDF5 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current methods for polysaccharide expression analysis (mass spectrometry, nuclear magnetic resonance, high performance liquid chromatography, etc.) have disadvantages such as complicated operation, expensive instruments, and high biodestructiveness. Therefore, we urgently need to invent a low-cost, easy-to-operate, high-sensitivity, Good biocompatibility detection method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of photo-induced chemical paper chip for detecting expression of polysaccharides on surfaces of cancer cells
  • Preparation of photo-induced chemical paper chip for detecting expression of polysaccharides on surfaces of cancer cells
  • Preparation of photo-induced chemical paper chip for detecting expression of polysaccharides on surfaces of cancer cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Application of paper-based photoelectrochemical devices in detecting the expression of polysaccharides on the surface of MCF-7 cells:

[0040] (1) Use Adobe illustrator CS4 software to design the printing pattern of the paper chip on the computer;

[0041] (2) Print the printing pattern designed in step (1) on No. 1 chromatographic paper with a wax printer, then place the A4 chromatographic paper with the wax pattern in a flat heater or an oven, and heat it at 130 ºC for 2 min , so that the wax melts and saturates the entire thickness of the paper, forming a hydrophobic wall;

[0042] (3) Print the carbon working electrode, Ag / AgCl reference electrode and carbon counter electrode on the wax-printed chromatographic paper obtained in step (2) by screen printing technology;

[0043] (4) After adding gold to the sample plate working area of ​​the chromatographic paper obtained in step (3), connect photoelectric materials zinc oxide, cadmium telluride quantum dots, and gold...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a photo-induced chemical paper chip for detecting expression of polysaccharides on surfaces of cancer cells. The preparation method comprises the following steps: designing lyophobic wax printing patterns of the paper chip by using Adobe illustrator CS4 software; printing the paper chip by using a wax printer; printing a working electrode, a reference electrode and a counter electrode by using a screen printing technique; growing gold nanoparticles in a working region, then connecting photoelectric material zinc oxide, cadmium telluride quantum dots and gold-grafted meso-porous silicon, modifying HRP-mdsDNA, and providing a built-in light source and binding sites of recognition cells for a photo-induced chemical system; and finally, adding a polysaccharides inhibitor, and detecting expression of the polysaccharides on the surfaces of the tumor cells by change of photocurrent response.

Description

technical field [0001] The invention relates to the technical fields of nanomaterial technology, hybridization chain reaction, paper chip technology, and polysaccharide expression detection on the surface of tumor cells, and more specifically relates to the construction of a paper-based analysis device suitable for in situ detection of polysaccharide expression on the surface of tumor cells. Background technique [0002] Glycosylation is a significant modification of proteins that can regulate protein functions. Polysaccharides are important structures on the cell surface and are closely related to cell migration and differentiation. The expression of polysaccharides on the cell surface is a dynamic process closely related to the cell environment and state. The abnormal expression of polysaccharides is not only related to many pathological phenomena but also a marker of many diseases. Therefore, detecting the expression of polysaccharides on the cell surface is of great si...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/327
CPCG01N27/327
Inventor 葛慎光兰飞飞梁琳琳张彦李丽杨红梅任娜于京华颜梅
Owner UNIV OF JINAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com