Bone repair composite for inducing mesenchymal stem cell differentiation and preparing method of bone repair composite

A composite material and mesenchymal stem cell technology, applied in the field of bone repair composite materials that induce mesenchymal stem cell differentiation and its preparation, can solve problems such as unsatisfactory biological properties, achieve good physical and chemical properties, reduce agglomeration effects, and promote adhesion Effect

Active Publication Date: 2016-10-12
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the technical problem of unsatisfactory biological performance of existing materials, and provides a bone repair composite material with ideal biological performance and simple processing method for inducing differentiation of mesenchymal stem cells and its preparation method

Method used

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  • Bone repair composite for inducing mesenchymal stem cell differentiation and preparing method of bone repair composite
  • Bone repair composite for inducing mesenchymal stem cell differentiation and preparing method of bone repair composite
  • Bone repair composite for inducing mesenchymal stem cell differentiation and preparing method of bone repair composite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Preparation of graphene oxide (GO)

[0040]Graphite oxide was successfully obtained by the optimized Hummers method, and uniformly dispersed single-layer or few-layer graphene oxide was obtained by ultrasound. The specific method is:

[0041] Mix natural flake graphite and sodium nitrate in a ratio of 2:1, add 230ml of concentrated sulfuric acid, then add potassium permanganate in ice bath, stir, and keep the reaction temperature below 20°C. Remove the ice bath, keep warm in a water bath at 35°C for 30 minutes, then slowly add 460ml of deionized water, wait until the reaction temperature drops to 70°C, and bathe at the same temperature for 10 minutes. Then add 700ml of hydrogen peroxide to terminate the reaction. Suction filtration, repeated washing and centrifugation of the obtained yellow-brown filtrate until the pH of the supernatant is about 7, and drying to obtain graphite oxide.

[0042] Ultrasonic separation: Mix graphite oxide and deionized water at a rat...

Embodiment 2

[0053] Steps (1), (2), (3) are the same as in Example 1.

[0054] (4) Graphene oxide / bioactive glass composite (DGO / BG) prepared by sol-gel method

[0055] The DGO / BG composite material with 1wt.% DGO was prepared by the sol-gel method. The preparation process was as follows: the experiment used ethyl orthosilicate (TEOS), triethyl phosphate (TEP), calcium nitrate tetrahydrate (CN), etc. 58S bioactive glass (BG) was prepared by sol-gel method. First, 0.36ml of TEP was dissolved in the mixed liquid of distilled water, absolute ethanol and ammonia water, and stirred thoroughly at 80°C for 24 hours to obtain a hydrolyzed TEP solution. Then 0.65ml TEOS and 0.35g CN were added into the TEP hydrolysis solution and stirred at room temperature for 120h to form a sol-gel solution. Weigh 0.0136 g of the DGO prepared in (3) in advance, dissolve it in dimethylformamide (DMF), and sonicate for 2 hours to form a uniform DGO suspension. The sol-gel solution was slowly added to the DGO sus...

Embodiment 3

[0060] Steps (1), (2), (3) are the same as in Example 1.

[0061] (4) Graphene oxide / bioactive glass composite (DGO / BG) prepared by sol-gel method

[0062] The DGO / BG composite material with 3wt.% DGO was prepared by the sol-gel method. The preparation process was as follows: the experiment used ethyl orthosilicate (TEOS), triethyl phosphate (TEP), calcium nitrate tetrahydrate (CN), etc., 58S bioactive glass (BG) was prepared by sol-gel method. First, 0.36ml of TEP was dissolved in the mixed liquid of distilled water, absolute ethanol and ammonia water, and stirred thoroughly at 80°C for 24 hours to obtain a hydrolyzed TEP solution. Then 0.65ml TEOS and 0.35g CN were added into the TEP hydrolysis solution and stirred at room temperature for 120h to form a sol-gel solution. Weigh 0.0415 g of the DGO prepared in (3) in advance, dissolve it in dimethylformamide (DMF), and sonicate for 2 hours to form a uniform DGO suspension. The sol-gel solution was slowly added to the DGO su...

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Abstract

The invention relates to a bone repair composite for inducing mesenchymal stem cell differentiation and a preparing method of the bone repair composite. The technical problem that existing materials are unsatisfactory in biological property is solved. The bone repair composite is formed by a graphene film and biologically-active glass, wherein the biologically-active glass is uniformly loaded on the surface of the graphene film and is in uniform spherical morphology. The bone repair composite and the preparing method can be applied to the field of bone repair.

Description

technical field [0001] The invention relates to a bone tissue engineering scaffold material and a preparation method thereof, in particular to a bone repair composite material capable of inducing differentiation of mesenchymal stem cells and a preparation method thereof. Background technique [0002] An ideal bone tissue engineering scaffold material can not only provide a three-dimensional space for the growth of osteoblasts, but also have osteoconductive and osteoinductive activities, guide the adhesion and proliferation of osteoblasts, and induce osteogenic differentiation of stem cells. The many unique and excellent properties of nanomaterials make them have broad application prospects in many fields, and nanomaterials with conductive properties have received unprecedented attention because of their special conductive properties. Through a series of processing, it can become a stimulus-responsive material with visual effects, a biorecognition film material with biosensor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/08A61L27/10A61L27/50
CPCA61L2430/02A61L27/08A61L27/10A61L27/50A61L2300/10A61L2300/216A61L2300/412A61L2300/45
Inventor 邓旭亮莫晓菊卫彦张学慧沈洋蔡晴
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
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