Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method for preparing alkali-tolerant xylanase by suaeda screening

A xylanase and alkali-resistant technology, which is applied in the field of preparation of alkali-resistant xylanase by screening Suaeda salsa, can solve the problems of alkali resistance and low enzyme activity, long production cycle, poor high temperature resistance, etc. Improve alkali resistance and enzyme activity, shorten production cycle, and good high temperature resistance

Inactive Publication Date: 2016-09-28
郭迎庆
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention: Aiming at the drawbacks of the alkaline xylanase currently produced, such as long production cycle, low yield, poor high temperature resistance, low alkali resistance and enzyme activity, and poor use effect, a kind of alkaline xylanase is provided. Take glucose, cellulose, etc., mix them, and sterilize them at high temperature to prepare a bacterial culture medium, inoculate Bacillus pumilus M-26, cultivate Bacillus pumilus colonies for later use, collect the stems and leaves of Suaeda salsa, grind them, and mix them with potassium sulfate and magnesium sulfate Sterilize to prepare blank Suaeda salsa medium, inoculate Bacillus pumilus colonies, repeat culture and screen to obtain alkali-resistant Bacillus pumilus colonies, filter to obtain filtrate, centrifuge to obtain supernatant, mix with ammonium sulfate, treat in ice water bath, and centrifuge to obtain the lower layer Precipitation, freeze-drying to prepare the method for alkali-resistant xylanase

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] First, in parts by weight, weigh 55 parts of deionized water, 20 parts of glucose, 10 parts of cellulose and 10 parts of magnesium sulfate and place them in a petri dish, stir and mix them and put them in a high-temperature sterilization process at 100°C for 10 minutes, statically Cool the prepared bacterial culture medium, then inoculate Bacillus pumilus M-26 into the above-prepared bacterial culture medium according to the inoculum size of 10%, place it at 37°C, and shake it at 200r / min for 2 days , cultivate Bacillus pumilus colony, standby; Then collect Suaeda salsa stem and leaf, clean and grind it into Suaeda salsa slurry, then weigh 55 parts of Suaeda salsa slurry, 5 parts of potassium phosphate and 5 parts of magnesium sulfate were stirred and mixed and placed in a petri dish. After sterilizing under ultraviolet light for 15 minutes, a blank Suaeda salsa medium was prepared; On the blank Suaeda salsa culture medium prepared above with the same quality, control t...

example 2

[0019] First, in parts by weight, weigh 65 parts of deionized water, 15 parts of glucose, 8 parts of cellulose and 8 parts of magnesium sulfate and place them in a petri dish, stir and mix them and put them in a high-temperature sterilization process at 100°C for 13 minutes, and statically Cool the prepared bacterial culture medium, then inoculate Bacillus pumilus M-26 into the above-prepared bacterial culture medium according to the inoculum size of 10%, place it at 37°C, and shake it at 210r / min for 3 days , cultivate Bacillus pumilus bacterium colony, standby; Then collect Suaeda salsa stem and leaf, clean and grind it into Suaeda salsa slurry, then weigh 65 parts of Suaeda salsa slurry, 8 parts of potassium phosphate and 7 parts of magnesium sulfate were stirred and mixed and placed in a petri dish. After sterilizing under ultraviolet light for 18 minutes, a blank Suaeda salsa medium was prepared; and then the cultured Bacillus pumilus colony was inoculated to 5 parts On t...

example 3

[0022] First, in parts by weight, weigh 75 parts of deionized water, 10 parts of glucose, 5 parts of cellulose and 5 parts of magnesium sulfate and place them in a petri dish, stir and mix them and place them at 100°C for high-temperature sterilization for 15 minutes. Cool the prepared bacterial culture medium, then inoculate Bacillus pumilus M-26 into the above-prepared bacterial culture medium according to the inoculum size of 10%, place it at 37°C, and shake it at 220r / min for 3 days , cultivate bacillus pumilus colonies, standby; then collect Suaeda salsa stem and leaf, clean and grind it into Suaeda salsa slurry, then weigh 75 parts of Suaeda salsa slurry, 10 parts of potassium phosphate and 10 parts of magnesium sulfate were stirred and mixed and placed in a petri dish. After sterilizing under ultraviolet light for 20 minutes, a blank Suaeda salsa medium was prepared; and then the cultured Bacillus pumilus colonies were inoculated to 5 parts according to the inoculum size...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing alkali-resistant xylanase by screening Suaeda salsa, and belongs to the technical field of alkali-resistant xylanase preparation. The present invention mixes glucose, cellulose, etc., prepares bacterial culture medium through high-temperature sterilization, inoculates Bacillus pumilus M-26, and cultivates Bacillus pumilus colonies for use. Blank Suaeda pumilus culture medium was prepared by mixed magnesium sterilization, inoculated with Bacillus pumilus colonies, repeatedly cultivated and screened to obtain alkali-resistant Bacillus pumilus colonies, filtered to obtain a filtrate, centrifuged to obtain a supernatant, mixed with ammonium sulfate, and treated in an ice-water bath, isolated The bottom layer of the experience was precipitated and freeze-dried to obtain alkali-resistant xylanase. The beneficial effects of the present invention are: the preparation steps of the present invention are simple, the production cycle is shortened by 11.2%, and the yield is increased by more than 25.6%; The effect is good.

Description

technical field [0001] The invention relates to a method for preparing alkali-resistant xylanase by screening Suaeda salsa, belonging to the technical field of alkali-resistant xylanase preparation. Background technique [0002] Xylanase is widely distributed in nature and can be obtained from animals, plants and microorganisms. For example, xylanases are found in marine and terrestrial bacteria, marine algae, fungi, yeasts, rumen and ruminant bacteria, snails, crustaceans, land plant tissues and various invertebrates. Microbial xylanases are ubiquitous in nature, and there are various types and applications in a wide range of fields. Therefore, there are many research reports on microbial xylanases. The xylanases from bacteria and fungi are the most researched and applied recently. Among them, bacteria can produce alkaline and acidic xylanases, while fungi can only produce alkaline xylanases. Among the fungi, filamentous fungi secrete the highest extracellular enzymes. A...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12R1/08
CPCC12N9/2482C12Y302/01008
Inventor 郭迎庆盛海丰高玉刚
Owner 郭迎庆
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com