Method for establishing Mongolian gerbil orthotopic liver transplantation model and method for separating hepatic stellate cells

A technology of hepatic stellate cells and long-clawed gerbils, applied in cell dissociation methods, animal cells, vertebrate cells, etc., to achieve the effect of fierce animal resistance, ideal effect, and reduction of biliary obstruction

Inactive Publication Date: 2016-09-21
ZHEJIANG ACAD OF MEDICAL SCI
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature at home and abroad on the application of long-clawed gerbils in organ transplantation, so this paper uses long-clawed gerbils as donors and recipients to build a good technical platform for the application of long-clawed gerbils in liver transplantation, filling the animal Gaps in organ transplantation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for establishing Mongolian gerbil orthotopic liver transplantation model and method for separating hepatic stellate cells
  • Method for establishing Mongolian gerbil orthotopic liver transplantation model and method for separating hepatic stellate cells
  • Method for establishing Mongolian gerbil orthotopic liver transplantation model and method for separating hepatic stellate cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] 1. Materials and methods

[0061] 1.1 Experimental animal: long-clawed gerbil, male, weighing 40-50g, provided by Zhejiang Experimental Animal Center, production license SCXK (Zhejiang) 2014-0001;

[0062] Breeding conditions: The experiment process was completed in a barrier system laboratory (air cleanliness of 10,000 grades, ventilation rate 10-20 times per hour, temperature 20-26°C, daily temperature difference ≤3°C, relative humidity 40-70%). Use license SYXK (Zhejiang) 2014-0008. The long-clawed gerbils were fed with 60Co sterilized nutrient compound feed, drank ultra-filtered water, and the cage utensils and litter were all sterilized by high pressure at 121°C for 20 minutes.

[0063] 1.2 Experimental equipment: ether (Hangzhou Chemical Reagent Factory), ketamine needle (100mg / 2ml, Hangzhou No. 1 Pharmaceutical Factory), heparin injection (5000U / 2ml, Hangzhou No. 1 Pharmaceutical Factory), microsurgical instruments (Shanghai Medical Instrument Factory), sutur...

Embodiment 2

[0086] 2. Materials and methods

[0087] 1.1 Experimental animal: long-clawed gerbil, male, weighing 40-50g, provided by Zhejiang Experimental Animal Center, production license SCXK (Zhejiang) 2014-0001;

[0088] Breeding conditions: The experiment process was completed in a barrier system laboratory (air cleanliness of 10,000 grades, ventilation rate 10-20 times per hour, temperature 20-26°C, daily temperature difference ≤3°C, relative humidity 40-70%). Use license SYXK (Zhejiang) 2014-0008. The long-clawed gerbils were fed with 60Co sterilized nutrient compound feed, drank ultra-filtered water, and the cage utensils and litter were all sterilized by high pressure at 121°C for 20 minutes.

[0089] 2.2 Experimental equipment: ether (Hangzhou Chemical Reagent Factory), ketamine needle (100mg / 2ml, Hangzhou No. 1 Pharmaceutical Factory), heparin injection (5000U / 2ml, Hangzhou No. 1 Pharmaceutical Factory), microsurgical instruments (Shanghai Medical Instrument Factory), sutur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
The inside diameter ofaaaaaaaaaa
Outer diameteraaaaaaaaaa
The inside diameter ofaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for establishing a Mongolian gerbil orthotopic liver transplantation model and a method for separating hepatic stellate cells. The method for establishing the Mongolian gerbil orthotopic liver transplantation model comprises the steps that a donor is narcotized, and a grafted liver is cut and taken out of the donor; a receptor is narcotized, the liver is cut out, the grafted liver is implanted into the receptor with the liver cut out; the survival rate of the Mongolian gerbil orthotopic liver transplantation model is calculated. Mongolian gerbil orthotopic liver transplantation is executed, changes of the Mongolian gerbil orthotopic liver transplantation model are observed, and the survival rate is calculated. The method for separating the hepatic stellate cells of Mongolian gerbil comprises the steps that chloral hydrate is used for intraperitoneal injection to narcotize the male Mongolian gerbil, or the male Mongolian gerbil which dead just now is selected, and the grafted liver is cut and taken out of the donor; the hepatic stellate cells are separated out of the grafted liver and obtained. According to the method for separating the hepatic stellate cells, the yield and survival rate of the hepatic stellate cells can be increased, and the purity of HSCs can be guaranteed.

Description

technical field [0001] The invention relates to the technical field of gerbil liver transplantation and cell separation, in particular to a method for establishing a model of orthotopic liver transplantation in a gerbil and a method for isolating hepatic stellate cells. Background technique [0002] A stable and reliable animal model of liver transplantation is the basis of clinical liver transplantation and immune tolerance research. Only when a mature and stable animal model is used as a technical platform, can the development of organ transplantation and immune tolerance research achieve a qualitative leap. Although the rat liver transplantation model is currently the most commonly used animal model, the stability and reproducibility of the closed group rat model needs to be further improved, while the stability of the inbred rat model has been improved, but due to lack of resources Relatively scarce, difficult to obtain, and relatively expensive, so it is very important...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01K67/027C12N5/071
CPCA01K67/0271A01K2267/03C12N5/067C12N2509/00
Inventor 楼琦石巧娟卢领群李巍郭红刚杜江涛应华忠
Owner ZHEJIANG ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap