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Preparation and application of bacillus altitudinis bacterial agent

A highlander bacillus and microbial agent technology, applied in the direction of application, fungicide, bacteria, etc., to achieve high application value, stable control effect, and low production cost

Active Publication Date: 2016-08-03
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are few reports on the control of postharvest diseases of peach by Bacillus altitudinis

Method used

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  • Preparation and application of bacillus altitudinis bacterial agent
  • Preparation and application of bacillus altitudinis bacterial agent
  • Preparation and application of bacillus altitudinis bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Isolation and screening of Bacillus highlander 14b

[0022] In the peach orchard without chemical fertilizers and pesticides, the rhizosphere soil of peach trees was collected, and the dilution separation method was used to make 10 -1 、10 -2 、10 -3 and 10-4 soil dilution, use a pipette to draw 10 -2 、10 -3 and 10 -4 Add 0.1mL of the soil dilution solution to the NA medium plate, spread it evenly with a sterile applicator, and mark the serial number. After standing for one hour, reverse the culture plate and cultivate it at 37°C. From the second day of culture, single bacterial colonies were selected according to the characteristics of the colony shape, and transplanted to NA medium respectively, and were used for subsequent experiments after 48 hours of culture. The purified strains were stored on NA slant medium at 4°C.

[0023] Antagonist bacteria were screened by plate confrontation method. Inoculate the pathogenic bacteria cake (8mm puncher) in the...

Embodiment 2

[0024] Embodiment 2: the preparation of microbial bacterial agent

[0025] The microbial bacterial agent used for preventing and treating postharvest fungal diseases of peaches includes fermentation broth and fermentation filtrate of Bacillus highlander 14b, and the preparation steps are as follows:

[0026] 1) Activated strain: transfer the preserved Bacillus uplander 14b to NA medium by streaking, and place in an incubator at 40°C for 24 hours of constant temperature and moisture cultivation;

[0027] 2) Preparation of seed solution: Pick a single colony of the antagonistic strain Bacillus upholsteria 14b that grew well on the NA medium and inoculate it into the NB liquid medium, culture it with shaking at 40°C for 48 hours, and the shaking frequency is 130-180r / min;

[0028] 3) Preparation of fermentation broth: inoculate the seed solution of strain 14b prepared in step 2) into NB liquid culture solution with an inoculum size of 3% by volume, and the charging amount is 50-1...

Embodiment 3

[0031] Embodiment 3: the influence of microbial bacterial agent on the growth of pathogenic bacteria mycelia

[0032] The microbial agent includes the fermentation liquid and the fermentation filtrate of Bacillus highlander 14b prepared in Example 2.

[0033] The filter paper method was used to measure the effect of strain 14b fermentation broth on mycelial growth. Inoculate the cakes of brown rot fungus, botrytis cinerea, soft rot fungus, and penicillium fungus in the center of the PDA plate respectively, dip a piece of sterile filter paper (Φ=8mm) into the fermentation broth of bio-control bacteria, and stick it symmetrically at a distance from the fungus cake of pathogenic bacteria Incubate at 26°C at 2.5 cm, observe and record the growth diameter of pathogenic bacteria after 3 days and calculate the inhibition rate, see Table 1 for details.

[0034] The plate method containing filtrate was used to measure the effect of fermentation broth on mycelia growth. After mixing t...

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Abstract

The invention relates to antagonistic bacteria used for preventing and treating fungal disease after peaches are picked and a microbial agent prepared from the bacterial strain. The invention provides bacillus altitudinis with the preservation number of CGMCC No.12180, microbial agent fermentation broth prepared from the bacillus altitudinis and application of fermentation filtrate in prevention and treatment of the fungal disease after the peaches are picked. The bacillus altitudinis disclosed by the invention is obtained by being separated from rhizosphere soil of peach trees in Feicheng peach gardens in which fertilizer and pesticides are not applied, can effectively prevent and treat multiple fungal diseases such as peach brown rot, gray mold, soft rot and penicilliosis, is a potential safe and ecological biological control bacterial strain with good biocontrol effect, no pollution and wide prevention and treatment range and has a broad market application prospect.

Description

(1) Technical field [0001] The invention relates to a bacillus uplander in the field of microbes and biological control and the fermented liquid and fermented filtrate of the microbial agent prepared therefrom, in particular to its application in preventing and controlling peach post-harvest diseases. (2) Background technology [0002] Peach is an important drupe fruit tree with a wide distribution and a large planting area in my country. With the increase of cultivated area and yield year by year, brown rot caused by Monilinia fructicola, gray mold caused by Botrytis cinerea, and soft rot caused by Rhizopus stolonifer The problems caused by Penicillium expansum and Penicillium expansum have become increasingly prominent, seriously affecting the storage, transportation and processing of peaches. Researchers at home and abroad are working hard to find new ways to replace or reduce chemical corrosion protection. However, the current control methods are mainly chemical contro...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 石晶盈殷晓慧王庆国张畅彭勇张欣刘佩
Owner SHANDONG AGRICULTURAL UNIVERSITY
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