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Three-dimensional capillary network biochip manufacturing method

A technology of capillaries and network biology, applied in the field of biomedicine, can solve the problems of cumbersome processing technology of microfluidic chips, and achieve the effect of low cost and simple process

Inactive Publication Date: 2016-07-13
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for manufacturing a three-dimensional capillary network biochip to solve the technical problems of traditional microfluidic chip processing technology

Method used

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Embodiment 3

[0025] In some embodiments, the manufacturing method of a three-dimensional capillary network biochip includes the following steps:

[0026] Step 1, configure polymer hydrogel poloxamer 407 with a concentration of 60wt / v% as a sacrificial polymer hydrogel, perform ultrasonic treatment to make it evenly mixed, and remove air bubbles inside by vacuuming. Then, the processed hydrogel was printed on the glass slide using a 3D bioprinter to obtain a sacrificial three-dimensional network scaffold that simulates capillaries. The needle diameter is 100 microns.

[0027] Step 2, filling curable liquid polydimethylsiloxane (PDMS) around the sacrificial three-dimensional network scaffold and between the hydrogel lines constituting the scaffold, after filling, vacuumize to remove excess air bubbles, Then solidify at room temperature without heating to avoid bubbles.

[0028] Step 3, after the PDMS to be filled is solidified, the three-dimensional network support is removed by soaking in...

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Abstract

A three-dimensional capillary network biochip manufacturing method includes steps: using a 3D (three dimensional) bio-printer for making macromolecular hydrogel into a blood capillary simulated sacrificial three-dimensional network stent; filling a solidifiable liquid-state biocompatible polymer material around the stent and between hydrogel lines forming the stent; after the liquid-state biocompatible polymer material is solidified, removing the stent to obtain a micro-fluidic chip with a hollow capillary network; planting endothelial cells in the capillary network of the micro-fluidic chip; culturing the endothelial cells to enable adherence growth of the endothelial cells to obtain capillaries through endothelialization, so that the micro-fluidic chip is made into a three-dimensional capillary network biochip.The three-dimensional capillary network biochip manufacturing method has the advantages of technical simplicity, low cost and the like.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a method for manufacturing a three-dimensional capillary network biochip based on 3D bioprinting. That is, a combination of bioprinting and tissue engineering is used to manufacture vascularized biochips, which are applied to drug screening and in vitro capillary disease mechanism research models. Background technique [0002] Bioprinting is a new biomanufacturing technology emerging after 2000. Its direct writing feature has advantages in the field of bioprocessing that traditional processing methods cannot match. It can use 3D modeling software to print out the designed structure layer by layer without other processes. [0003] The purpose of 3D microfluidic biochip technology is to integrate the entire analysis process from sample preparation, biochemical reaction to detection into a total micro analysis system (μTAS), that is, lab-on-a-chip. The traditional design method is to de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
CPCC12M23/16C12M25/10
Inventor 李勃朱朋飞许国军王进周济
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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