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Polyethylene glycol bipolymer with endothelial cell selectivity and preparation method and application method of composite coating

A polyethylene glycol and binary copolymer technology, which is applied in the interdisciplinary field, can solve the problems of reducing the migration and proliferation ability of endothelial cells, increasing the risk of late thrombosis, and destroying the vascular endothelial layer, so as to achieve the realization of in situ endothelial cells in vivo. Capture capability, maintain non-specific impedance performance, wide range of effects

Inactive Publication Date: 2016-06-29
HUANGHUAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, while the drugs carried by drug-eluting stents treat restenosis caused by excessive proliferation of smooth muscle cells, they also reduce the migration and proliferation of endothelial cells, destroying the endothelial layer of blood vessels, and increasing the risk of late thrombosis
Delayed endothelialization and late thrombosis caused by non-selective inhibitory effects of drugs are still key issues restricting its application

Method used

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  • Polyethylene glycol bipolymer with endothelial cell selectivity and preparation method and application method of composite coating
  • Polyethylene glycol bipolymer with endothelial cell selectivity and preparation method and application method of composite coating
  • Polyethylene glycol bipolymer with endothelial cell selectivity and preparation method and application method of composite coating

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Preparation of Endothelial Cell Selective Polyethylene Glycol Binary Copolymer

[0045]

[0046] (1.1) Dissolve 0.5 g of the biocompatible monomer PEGMA (n=19) of the cell glycocoating biomimetic, and 1.5 g of the polymerizable monomer GMA containing reactive functional groups with 30 ml of ethanol to obtain solution A;

[0047] (1.2) 0.04g initiator AIBN is added in solution A to obtain solution B;

[0048] (1.3) Deoxygenate solution B with argon for 30 minutes, heat to 60°C, and react for 24 hours;

[0049] (1.4) The solvent was removed under reduced pressure, and the glacial ether was precipitated twice to obtain the polyethylene glycol binary copolymer PEGMA-GMA with endothelial cell selectivity. NMR results confirmed that the obtained product had the expected structure. Such as figure 1 (a) shown.

[0050] (2) Preparation of Endothelial Cell Selective Polyethylene Glycol Composite Coating

[0051](2.1) Dissolving the endothelial cell-selective polyethyl...

Embodiment 2

[0061] (1) Preparation of Endothelial Cell Selective Polyethylene Glycol Binary Copolymer

[0062]

[0063] (1.1) Dissolve 0.8 g of the biocompatible monomer PEGMA (n=6) of the cell glycocoating biomimetic, and 1.6 g of the polymerizable monomer GMA containing reactive functional groups in 50 ml of ethanol to obtain solution A;

[0064] (1.2) 0.048g initiator AIBN is added in solution A to obtain solution B;

[0065] (1.3) Deoxygenate solution B with argon for 20 minutes, heat to 70°C, and react for 36 hours;

[0066] (1.4) The solvent was removed under reduced pressure, and the glacial ether was precipitated twice to obtain the polyethylene glycol binary copolymer PEGMA-GMA with endothelial cell selectivity. NMR results confirmed that the obtained product had the expected structure. Such as figure 1 (b) shown.

[0067] (2) Preparation of Endothelial Cell Selective Polyethylene Glycol Composite Coating

[0068] (2.1) Dissolving the endothelial cell-selective polyethyle...

Embodiment 3

[0078] (1) Preparation of Endothelial Cell Selective Polyethylene Glycol Binary Copolymer

[0079]

[0080] (1.1) Dissolve 2 g of the biocompatible monomer PEGMA (n=30) of the cell glycoside biomimetic, and 1 g of the polymerizable monomer GMA containing reactive functional groups with 70 ml of ethanol to obtain solution A;

[0081] (1.2) Add 0.01g initiator AIBN to solution A to obtain solution B;

[0082] (1.3) Deoxygenate solution B with argon for 20 minutes, heat to 80°C, and react for 48 hours;

[0083] (1.4) The solvent was removed under reduced pressure, and the glacial ether was precipitated twice to obtain the polyethylene glycol binary copolymer PEGMA-GMA with endothelial cell selectivity. NMR results confirmed that the obtained product had the expected structure.

[0084] (2) Preparation of Endothelial Cell Selective Polyethylene Glycol Composite Coating

[0085] (2.1) Dissolving the endothelial cell-selective polyethylene glycol binary copolymer PEGMA-GMA in ...

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Abstract

The invention relates to a polyethylene glycol bipolymer with endothelial cell selectivity and a preparation method and an application method of a composite coating. The polyethylene glycol bipolymer is synthesized by a cellular carbohydrate coat-bionic biocompatible monomer and a polymerizable monomer containing reactive active functional groups through a free radical polymerization method, and bioactive molecules for promoting endothelial cell adhesion is introduced through a surface fixing method to enable the coating to have endothelial cell selectivity. The polyethylene glycol composite coating with excellent reactivity is capable of keeping non-specificity impedance performance of stent surfaces and achieving in-vivo in-situ endothelial cell capture capability, is stable in structure and can be applicable to an internal environment of a human body, thereby having a promising prospect on cardiovascular restenosis, cancers and the like.

Description

technical field [0001] The technical field of the present invention is the interdisciplinary field of materials, biology, physics, chemistry and other subjects, and relates to a kind of endothelial cell selective polyethylene glycol binary copolymer and the preparation method of the copolymer. In addition, the present invention also relates to The application and application method of the binary copolymer in preparing composite coating. Background technique [0002] The widespread use of drug-eluting stents provides a good means for efficient and minimally invasive treatment of cardiovascular diseases. However, while the drugs carried by drug-eluting stents treat restenosis caused by excessive proliferation of smooth muscle cells, they also reduce the migration and proliferation of endothelial cells, destroying the endothelial layer of blood vessels, and increasing the risk of late thrombosis . Delayed endothelialization and late thrombosis caused by the non-selective inhi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F283/06C08F220/32C08F220/28A61L31/10A61L31/14A61L31/16
CPCA61L31/10A61L31/14A61L31/16A61L2300/42A61L2420/02C08F220/28C08F220/286C08F283/065C08L71/02C08F220/325
Inventor 魏雨张景迅冯先涛崔运启赵梦溪
Owner HUANGHUAI UNIV
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