Extracting method for polyphenol and flavone in aronia melanocarpa

A technology of Aronia nigra and an extraction method, which are applied in directions such as pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., can solve the problem of uneven distribution of ultrasonic energy, unfavorable extraction efficiency of active ingredients, insufficient extraction efficiency, etc. problems, to achieve the effect of improving comprehensive utilization efficiency, easy process scale-up and continuous operation, accurate and reliable process parameters

Inactive Publication Date: 2016-06-29
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ultrasonic-assisted extraction technology also has shortcomings, that is, during the ultrasonic extraction process, due to the uneven distribution of ultrasonic energy, cavitation effects are only generated in a small area near the ultrason

Method used

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  • Extracting method for polyphenol and flavone in aronia melanocarpa
  • Extracting method for polyphenol and flavone in aronia melanocarpa
  • Extracting method for polyphenol and flavone in aronia melanocarpa

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specific Embodiment approach

[0041] In order to further illustrate the technical solution of the present invention, in conjunction with the accompanying drawings, the specific implementation of the present invention is as follows:

[0042] The invention adopts an ultrasonic-assisted two-phase aqueous system to extract polyphenols and flavonoids in Arrowberry pomace, and uses the yield of polyphenols and the yield of flavonoids as evaluation indexes to determine the process parameters of the ultrasonic-assisted two-phase extraction technology. The specific process route is as follows:

[0043] Raw material processing→preparation of two-phase aqueous system→ultrasonic extraction→centrifugation→sample solution of polyphenols and flavonoids from Sorbus arvensis pomace→concentration under reduced pressure→storage

Embodiment 1

[0046] a) Raw material handling

[0047] Thawing the frozen fruit of Sorbus arvensis at room temperature, cleaning, squeezing the juice, drying the pomace, crushing, and passing through a 60-mesh sieve, and storing the prepared 60-mesh Sorbus arvensis pomace powder in a dry environment, spare;

[0048] b) Preparation of two-phase aqueous system

[0049] According to the concentration of ammonium sulfate (the ratio of the mass of ammonium sulfate to the volume of distilled water) is 0.324g / mL, and the ratio of alcohol to water (the ratio of the volume of absolute ethanol to distilled water) is 0.69, an ethanol / ammonium sulfate two-phase system is established;

[0050] Weigh 0.50 g of the Arrowberry pomace powder in step a) and add it into the ethanol / ammonium sulfate two-phase system according to the material-to-water ratio (ratio of material mass to distilled water volume) as 1:20 g / mL, fully shake and seal;

[0051] c) Ultrasonic extraction:

[0052] The two-phase aqueous...

Embodiment 2

[0062] a) Raw material handling:

[0063] Thaw the frozen fruit of Sorbus arvensis at room temperature, wash, squeeze the juice, dry the pomace, crush it, and pass through a 60-mesh sieve, and store the prepared 60-mesh Sorbus arvensis pomace powder in a dry environment for later use ;

[0064] b) Preparation of two-phase aqueous system:

[0065] According to the concentration of ammonium sulfate (the ratio of the mass of ammonium sulfate to the volume of distilled water) is 0.320g / mL, and the ratio of alcohol to water (the ratio of the volume of absolute ethanol to distilled water) is 0.71, establish an ethanol / ammonium sulfate two-phase system;

[0066] Weigh 0.50 g of the Arrowberry pomace powder in step a) and add it into the ethanol / ammonium sulfate two-phase system according to the material-to-water ratio (ratio of material mass to distilled water volume) as 1:20 g / mL, fully shake and seal;

[0067] c) Ultrasonic extraction:

[0068] The two-phase aqueous system that...

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Abstract

The invention relates to the technology of natural product extraction, and particularly relates to an extraction method for polyphenol and flavone in aronia melanocarpa. Aronia melanocarpa residues are used as raw materials, an ethyl alcohol/ammonium sulfate aqueous two-phase system is used as an extracting agent, and an ultrasonic assistance aqueous two-phase technology is applied to extracting polyphenol and flavone in aronia melanocarpa residues. The novel technology is provided for extracting polyphenol and flavone in aronia melanocarpa, the method has the advantages that phase splitting is rapid, equipment is easy to operate, the extracting process is rapid, the extracting efficiency is high, extracting conditions are accurate and reliable, the production cost of polyphenol and flavone in aronia melanocarpa is lowered, and industrial production is achieved.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting polyphenols and flavonoids from Aronia nigra. Background technique [0002] Aroniamelanocarpa is a perennial deciduous shrub belonging to the genus Aronia melanocarpa in the family Rosaceae. At present, this tree species is widely cultivated in North America, Eastern European countries, the three northeastern provinces of my country, Inner Mongolia, Xinjiang, Hebei, Henan, Beijing, Shandong and other places. Sorbus adenocarpa mainly produces fruit, and the fresh fruit yield can reach 10-15t / hm 2 . The fruit is rich in polyphenols, organic acids, triterpenes, vitamins and other ingredients, among which the total amount of polyphenols reaches 2.5% to 3.5%, which is higher than that of blueberries, lingonberries and cranberries. The fruit of Aronia nigra is not only a natural source of food coloring, but also a raw material f...

Claims

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Application Information

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IPC IPC(8): A61K36/73
Inventor 徐艳阳仇洋张铁华具东虎蒋风雷任辉刘辉霍冰洋王雪松孙竹
Owner JILIN UNIV
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