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Protein removal method for hericium erinaceus polysaccharide extracting solution

A technology of Hericium erinaceus polysaccharide and extracting solution, which is applied in the field of deproteinization of polysaccharide extracting solution, can solve the problems of potential safety hazards, affecting the structure and activity of polysaccharides, residual organic solvent, etc., so as to ensure the structure and activity and avoid the breaking of glycosidic bonds. Effect

Inactive Publication Date: 2016-06-22
GUANGDONG APOLLO GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hydrochloric acid method and sodium hydroxide method are prone to hydrolysis of polysaccharides due to the use of acid and alkali, and some glycosidic bonds are broken, which affects the structure and activity of polysaccharides.
The Sevage method, the trichloroacetic acid method, the trifluorotrichloroethane method, etc. all use organic solvents, which easily cause the residue of organic solvents, and the chloroform and n-butanol used in the Sevage method and the trichloroacetic acid method used Trichloroacetic acid is a toxic chemical reagent, which is not conducive to human health and has potential safety hazards, so it is not suitable for the development of food products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of deproteinization method of Hericium erinaceus polysaccharide extract of the present embodiment, it comprises the following steps:

[0029] Step 1, preparing chitosan-acetic acid solution: dissolving chitosan in acetic acid solution, stirring evenly, promptly makes chitosan-acetic acid solution; in the present embodiment, the volume percentage concentration of acetic acid in the acetic acid solution is 1% , the mass percent concentration of chitosan in the chitosan-acetic acid solution is 2.5%;

[0030] Step 2, mixing Hericium erinaceus polysaccharide extract with chitosan-acetic acid solution: Mix Hericium erinaceus polysaccharide extract with the chitosan-acetic acid solution prepared in step 1 using a vortex mixer to obtain the first mixture; In the present embodiment, the volume ratio of Hericium erinaceus polysaccharide extract and chitosan-acetic acid solution is 50:1;

[0031] Wherein, the polysaccharide extract of Hericium erinaceus in step 2 is the fr...

Embodiment 2

[0036] A kind of deproteinization method of Hericium erinaceus polysaccharide extract of the present embodiment, it comprises the following steps:

[0037] Step 1, preparing chitosan-acetic acid solution: dissolving chitosan in acetic acid solution, stirring evenly, promptly makes chitosan-acetic acid solution; in the present embodiment, the volume percentage concentration of acetic acid in the acetic acid solution is 0.5% , the mass percent concentration of chitosan in the chitosan-acetic acid solution is 1%;

[0038]Step 2, mixing Hericium erinaceus polysaccharide extract with chitosan-acetic acid solution: Mix Hericium erinaceus polysaccharide extract with the chitosan-acetic acid solution prepared in step 1 using a vortex mixer to obtain the first mixture; In the present embodiment, the volume ratio of Hericium erinaceus polysaccharide extract and chitosan-acetic acid solution is 40:1;

[0039] Wherein, the polysaccharide extract of Hericium erinaceus in step 2 is the fru...

Embodiment 3

[0044] A kind of deproteinization method of Hericium erinaceus polysaccharide extract of the present embodiment, it comprises the following steps:

[0045] Step 1, preparing chitosan-acetic acid solution: dissolving chitosan in acetic acid solution, stirring evenly, promptly makes chitosan-acetic acid solution; in the present embodiment, the volume percentage concentration of acetic acid in the acetic acid solution is 3% , the mass percent concentration of chitosan in the chitosan-acetic acid solution is 2%;

[0046] Step 2, mixing Hericium erinaceus polysaccharide extract with chitosan-acetic acid solution: Mix Hericium erinaceus polysaccharide extract with the chitosan-acetic acid solution prepared in step 1 using a vortex mixer to obtain the first mixture; In the present embodiment, the volume ratio of Hericium erinaceus polysaccharide extract and chitosan-acetic acid solution is 60:1;

[0047] Wherein, the polysaccharide extract of Hericium erinaceus in step 2 is the frui...

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Abstract

The invention relates to the technical field of protein removal for hericium erinaceus polysaccharide extracting solutions, in particular to a protein removal method for a hericium erinaceus polysaccharide extracting solution.The method comprises the steps that 1, a chitosan-acetic acid solution is prepared; 2, the hericium erinaceus polysaccharide extracting solution is mixed with the chitosan-acetic acid solution; 3, centrifugal treatment is carried out for the first time; 4, centrifugal treatment is carried out for the second time.The protein removal method for the hericium erinaceus polysaccharide extracting solution has the advantages of being safe, nontoxic, free of pollution, environmentally friendly, high in protein removal rate and low in polysaccharide losing rate, glycosidic bond breakage can be avoided, and then the polysaccharide structure and activity can be ensured.In addition, the protein removal method for the hericium erinaceus polysaccharide extracting solution has the advantages of being simple in treatment, easy to operate, low in production cost and applicable to large-scale production, and can be applied to production of relevant food health care products.

Description

Technical field [0001] The invention involves the technical field of protein dehydration of polysaccharides, especially the protein method that involves a polysaccharide extraction solution. Background technique [0002] Hericiumerinaceus is precious medicated diet and bacteria.Modern studies have shown that Hericium mushrooms contain a variety of active ingredients, such as polysaccharides, crickets, and phenolic compounds.Among them, the main ingredients with functional activity in Herrye head mushrooms are polysaccharides of Hericium mushrooms.There are relatively many studies on Hericium mushroom polysaccharides. Studies have shown that it has physiological activity such as immune regulation, anti -tumor, and stomach protection, and the drugs and health products made of polysaccharides made of Hericium mushrooms are also loved by people and have broad development prospects.However, most of the health products related to Herrye head mushrooms are made of sapiexee extraction li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 周丹丹易晓敏周春晖黄惠华马炳南
Owner GUANGDONG APOLLO GRP
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