Preparation method of cobalt sulfide nanomaterial and method for detecting hydrogen peroxide with cobalt sulfide nanomaterial

Abstract

The invention relates to the field of food detection, in particular to a preparation method of a cobalt sulfide nanomaterial and a method for detecting hydrogen peroxide with the cobalt sulfide nanomaterial. O-dichlorobenzene, oleic acid and trioctyl phosphine oxide react with an o-dichlorobenzene solution containing carbonyl cobalt, then reaction products react with an o-dichlorobenzene solution containing sulfur, the reaction products are sequentially washed and centrifuged with methanol and distilled water repeatedly, and cobalt sulfide nanomaterial powder is obtained. According to the method for detecting hydrogen peroxide with the cobalt sulfide nanomaterial, to be specific, a phosphate buffer solution of cobalt sulfide is added to a Tirs-Hcl buffer solution, then a to-be-detected solution and a TMB color developing agent are added, and whether the to-be-detected solution contains hydrogen peroxide or not is judged through observation of color reaction. The method is high in detection speed and accuracy and has better specificity to detection of hydrogen peroxide.

Description

technical field [0001] The invention relates to the field of food detection, in particular to a method for preparing a cobalt sulfide nanometer material and detecting hydrogen peroxide. Background technique [0002] At present, the detection methods of hydrogen peroxide in food include titration analysis method (Huang Weikun. Food Inspection and Analysis [M]. Beijing: China Light Industry Press, 1989.171), high performance liquid chromatography-ultraviolet detection method (Chen Yihui, Liu Yan, Zhou Jianli , Zhu Haiwei, Xiang Lixia. Determination of hydrogen peroxide in food by high performance liquid chromatography-ultraviolet detection. Spectroscopic Laboratory Journal. 2009.26), spectrophotometry (Fan Huafeng, Zhang Zhongyi, Liu Zhenlin. Spectrophotometry for the determination of hydrogen peroxide in food [J]. Chinese Journal of Health Inspection. 2006.16), fluorescence method (Zhang Dexin, Cheng Luping, Yao Qunfeng. Fluorescence determination of residual hydrogen peroxid...

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Problems solved by technology

[0003] In the method of colorimetric analysis to detect hydrogen peroxide, the earliest catalyst used is hydrogen peroxide bioenzyme (HRP), which has good catalytic performance, high sensitivity and strong specificity. Extremely high and easily deactivated, coupled with high price, thus limiting its further application

In 2007, Gao et al. found that Fe 3 o 4 Magnetic nanomaterials have catalase activity, but Fe 3 o 4 Need to react at 40°C for 10-30min to get the result, and the detection limit is high

Although Co 3 o 4 Nanoflower, WS 2 Nanosheets and FeHPO 4 The stability and specificity of nanomaterials such as nanoflowers have been improved, and the detection limit is low, but the strict requirements on reaction time, temperature and pH limit the rapid detection of practical samples, such as WS 2 and Co 3 o 4 The detection limits of hydrogen peroxide are 2.9 μmol / L and 6 μmol / L respectively, and the reaction needs to be carried out at a temperature of 40-50°C and a weak acid condition with a pH value between 3-5, and the reaction time is about 20 minutes ; ferric hydrogen phosphate nanomaterials need to be under the condition of 80 ℃ to have high activity for colorimetric analysis

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