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Method for reinforcing plant-microorganism symbiosis system through bio-surfactant and application of system to in-situ repair of uranium polluted soil

A bio-surface and symbiotic system technology, applied in the field of uranium-contaminated soil remediation, can solve the problems of uranium remediation plants being difficult to absorb, having a large impact on remediation plants, and a long growth cycle of plants, etc. The effect of a short growth cycle

Active Publication Date: 2016-06-22
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, phytoremediation also has certain limitations, such as limited tolerance of remediation plants to uranium, longer plant growth cycle, greater impact of external environmental factors on remediation plants, and some fixed and precipitated uranium remediation plants are not easy to absorb, etc.
Many scholars have strengthened phytoremediation by adding chemical substances. Although the effect is obvious, the cost of restoration is increased, the environmental risk is high, and the added chemical substances are likely to cause secondary pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of culture substrate

[0035] Mix 1kg of uranium-free contaminated soil with 3kg of vermiculite and 0.8kg of peat soil after natural air-drying and sieving, mix evenly, and sterilize in high-pressure steam at 120°C for 2 hours to obtain the required culture substrate ;

[0036] (2) Preparation of biosurfactant strain liquid

[0037] The activated sludge in the aeration tank of the sewage treatment plant is boiled and filtered. 100g of ground corn stalks and 150g of grain bran were used as initial materials for composting, buried in 1kg of treated activated sludge, and 25g of air-dried and crushed chaff was added as a filler to improve the porosity of the compost. After the compost was cultivated at 10°C for 15 days, it was stored for later use.

[0038] Take 10g of compost samples and place them in a 250mL Erlenmeyer flask filled with 90mL of sterile water, vibrate for 25min, then stand still for 30min to obtain a soil suspension. Take 2mL of soil su...

Embodiment 2

[0045] (1) Preparation of culture substrate

[0046] Mix 1.5kg of uranium-free contaminated soil with 4.5kg of vermiculite and 0.65kg of peat soil after natural air-drying and sieving, mix well, and sterilize in high-pressure steam at 120°C for 2 hours to obtain the required Culture substrate;

[0047] (2) Preparation of biosurfactant strain liquid

[0048] The activated sludge in the aeration tank of the sewage treatment plant is boiled and filtered. 100g of ground corn stalks and 150g of grain bran were used as initial materials for composting, buried in 1kg of treated activated sludge, and 25g of air-dried and crushed chaff was added as a filler to improve the porosity of the compost. After the compost was cultivated at 10°C for 15 days, it was stored for later use.

[0049] Take 10g of compost samples and place them in a 250mL Erlenmeyer flask filled with 90mL of sterile water, vibrate for 25min, then stand still for 30min to obtain a soil suspension. Take 2mL of soil ...

Embodiment 3

[0056] (1) Preparation of culture substrate

[0057] Mix 2kg of uranium-free contaminated soil with 1.5kg of vermiculite and 0.5kg of peat soil after natural air-drying and sieving, mix evenly, and sterilize in high-pressure steam at 120°C for 2 hours to obtain the required culture. matrix;

[0058] (2) Preparation of biosurfactant strain liquid

[0059] The activated sludge in the aeration tank of the sewage treatment plant is boiled and filtered. 100g of ground corn stalks and 150g of grain bran were used as initial materials for composting, buried in 1kg of treated activated sludge, and 25g of air-dried and crushed chaff was added as a filler to improve the porosity of the compost. After the compost was cultivated at 10°C for 15 days, it was stored for later use.

[0060] Take 10g of compost samples and place them in a 250mL Erlenmeyer flask filled with 90mL of sterile water, vibrate for 25min, then stand still for 30min to obtain a soil suspension. Take 2mL of soil sus...

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Abstract

The invention relates to a method for reinforcing a plant and microorganism symbiosis system through bio-surfactant and application of the system to in-situ repair of uranium polluted soil. Uranium-pollution-free soil, vermiculite and peat soil serve as culture media, ricinus communis serves as a soil host plant, arbuscular mycorrhizae fungi serve as inoculation microorganisms, at first, a seedling culture device is utilized for culturing ricinus communis seedlings, ricinus communis seeds are inoculated with the arbuscular mycorrhizae fungi, a ricinus communis seedling-mycorrhizae fungus symbiosis system is formed, then bio-surfactant strain liquid is used for reinforcing the ricinus communis seedling-mycorrhizae fungus symbiosis system, and the ricinus communis seedling-mycorrhizae fungus symbiosis system with the bio-surfactant reinforcing function is obtained. The system is applied to in-situ repair of the uranium polluted soil. In the process of in-situ repair of the uranium polluted soil, the plant grows well, moreover, after the soil is repaired, the content of uranium in the plant is high, and the repair effect is good.

Description

technical field [0001] The invention belongs to the field of remediation of uranium-contaminated soil, and in particular relates to a method for strengthening a plant-microbe symbiosis system with a biosurfactant and its application in in-situ remediation of uranium-contaminated soil. Background technique [0002] Soil is an important part of the ecological environment. It is a complex and open material system between the living world and the non-biological world on which human beings depend. It is also a biogeochemical system that can support the growth and reproduction of plants, animals and microorganisms. Cyclic repository. With the rapid development of my country's nuclear industry and nuclear energy, the use of uranium is still increasing every year, resulting in a lot of uranium ore waste. According to incomplete statistics, a uranium mine with an annual output of 100,000 tons will produce 400,000 to 500,000 tons of uranium waste rock, of which the uranium content is...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10
CPCB09C1/10B09C1/105B09C2101/00
Inventor 肖方竹彭国文符建文石志科余丽梅蒲移秋
Owner NANHUA UNIV
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