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Method for rapidly identifying fruiting potential of edible fungus strains

A technology for edible mushrooms and strains, applied in the directions of botanical equipment and methods, applications, mushroom cultivation, etc., can solve the problems that mushroom bags cannot fruit normally, heavy workload, and complicated operation, so as to promote the production and development of edible mushrooms. , Improve work efficiency and simple operation

Inactive Publication Date: 2016-06-22
NINGDE NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the actual production of edible fungi, it often happens that due to the nature of the bacteria, the fungus bag cannot produce mushrooms normally, causing huge economic losses to the growers. Small-scale cultivation and fruiting experiments to evaluate the fruiting potential of the strains, so as to avoid the huge risks that may be brought about in cultivation
[0005] The fruiting potential of traditional edible fungus strains is usually evaluated by substituting material substrate cultivation method, that is, small-scale fruiting experiments are carried out using wood chips, cottonseed hulls, bagasse, bran, gypsum, etc. Obvious shortcomings such as heavy workload, long cycle, and low efficiency cannot realize the rapid and efficient evaluation of the fruiting potential of edible fungus strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The fruiting potential identification of embodiment 1 shiitake hybrid strain

[0019] 1) Weigh 200g miscellaneous sawdust and 50g bran, boil in 1L tap water for 20min, filter, add water to the filtrate to 1L, and use it as a medium;

[0020] 2) Put 150mL of culture medium in a 250mL Erlenmeyer flask, and place a 4cm×4cm×2cm heat-resistant sponge in the bottle, seal it with 8 layers of gauze, and sterilize it in an autoclave at 121°C for 20min ;

[0021] 3) After the culture medium is cooled, cut 2 pieces of strains with a size of 1cm×1cm from the inclined surface of the activated shiitake mushroom hybrid strain, one of which is inoculated into the medium to make it grow in large quantities, and the other is inoculated in the center of the sponge surface location, as a support point for primordium or fruiting body formation;

[0022] 4) Incubate the inoculated bottle on a shaker at 28°C and 160r / min for 5 days;

[0023] 5) After the shaker culture, put the inoculatio...

Embodiment 2

[0025] The fruiting potential identification of a certain wild woody fungus isolate bacterial strain of embodiment 2

[0026] 1) Weigh 200g miscellaneous sawdust and 50g bran, boil in 1L tap water for 20min, filter, add water to the filtrate to 1L, and use it as a medium;

[0027] 2) Put 150mL of the culture medium in a 500mL wide-mouth bottle, and place a heat-resistant sponge with a size of 4cm×4cm×2cm in the bottle, seal it with 8 layers of gauze, and sterilize it in an autoclave at 121°C Bacteria 20min;

[0028] 3) After the culture medium is cooled, cut 2 pieces of strains with a size of 1cm×1cm from the inclined surface of the activated wild woody fungus isolate, one of which is inoculated into the medium to make it grow in large quantities, and the other is inoculated in the The central position of the sponge surface, which serves as a support point for primordium or fruiting body formation;

[0029] 4) Incubate the inoculated bottle on a shaker at 28°C and 160r / min...

Embodiment 3

[0032] Example 3 Identification of fruiting potential of Pleurotus spp.

[0033] 1) Weigh 200g miscellaneous sawdust and 50g bran, boil in 1L tap water for 20min, filter, add water to the filtrate to 1L, and use it as a medium;

[0034] 2) Put 150mL of the culture medium in a 500mL wide-mouth bottle, and place a heat-resistant sponge with a size of 4cm×4cm×2cm in the bottle, seal it with 8 layers of gauze, and sterilize it in an autoclave at 121°C Bacteria 20min;

[0035] 3) After the culture medium is cooled, cut 2 pieces of bacteria with a size of 1cm×1cm from the activated Pleurotus inocula seed slope, one of which is inoculated into the medium to make it grow in large quantities, and the other is inoculated on the surface of the sponge The central position of the center, as the supporting point for the formation of primordia or fruiting bodies;

[0036] 4) Incubate the inoculated bottle on a shaker at 28°C and 160r / min for 5 days;

[0037] 5) After the shaker cultivat...

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Abstract

The invention discloses a method for rapidly identifying the fruiting potential of edible fungus strains. An extracting solution obtained after sawdust and bran water extraction serves as a culture medium and then is charged into a wild-mouth bottle or a triangular flask, heat-resisting sponge with the specification of 4 cm * 4 cm * 2 cm is put into the bottles, two strain blocks with the specifications of 1 cm * 1 cm are respectively inoculated in the central positions of the culture medium and the sponge surface after high-temperature sterilization, shaking cultivation is performed for 5 days, then standing cultivation is performed for 10-12 days, and it is observed that primordial or young fruiting bodies can be formed at sponge inoculation positions. The edible fungus strains can be rapidly identified by adopting the method, especially the fruiting potential of lignicolous edible fungus strains, and the method can be applied to identification of the fruiting potential of wild edible fungi, hybrid strains and introduced strains before large-scale cultivation and fruiting tests and has a great significance on the promotion of production and development of edible fungi.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a method for quickly identifying the fruiting potential of edible fungus strains. Background technique [0002] Edible fungi are a class of large fungi that can produce edible fruiting bodies. They are rich in nutrients such as proteins, polypeptides, amino acids, carbohydrates, nucleotides, cellulose, trace elements, and vitamins. Food that people love. In addition to being a food with high nutritional value, edible fungi such as Ganoderma lucidum and shiitake mushrooms also have various pharmacological functions such as immune regulation, anti-oxidation, liver protection, anti-cancer, tumor cell inhibition, and anti-aging. Due to the high medical and food value of edible fungi and the continuous improvement of people's health awareness, the market demand for edible fungi continues to increase. Therefore, the production of edible fungi has great po...

Claims

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Application Information

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IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 张维瑞刘盛荣苏贵平匡云波周修赵
Owner NINGDE NORMAL UNIV
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