Grape wine direct-adding type fermentation agent and preparation method thereof and fermentation method of grape wine
A technology of direct input starter and wine, applied in the field of wine fermentation, which can solve the problem of low nutrient content of dry red wine
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Embodiment 1
[0051] Saccharomyces cerevisiae CICC31089 was added to 0.9% physiological saline sterilized and cooled to 28°C, shaken and dissolved, and left to activate in a 28°C incubator for 30 minutes; the activated Saccharomyces cerevisiae was inoculated into the culture medium in the Erlenmeyer flask, Cultivate in a shaker at 28°C for 36 hours, and the shaker speed is 180 rpm to obtain a Saccharomyces cerevisiae mother starter; the medium includes 1.0L of 5°Bé wort;
[0052] Add 2wt% liquid glycerin, 2wt% xylooligosaccharides, and 2wt% sucrose to 10wt% skim emulsion, cool to 28°C after sterilization, inoculate Saccharomyces cerevisiae mother starter into it, and cultivate to live bacteria at 28°C Number ≥ 10 9 cfu / mL; The obtained strains were quick-frozen at -30°C, and then freeze-dried in a freeze dryer to obtain a freeze-dried powder of Saccharomyces cerevisiae.
[0053] Logger's yeast freeze-dried powder is prepared according to the following method:
[0054] Add Loggeriae CICC32...
Embodiment 2
[0069] The lyophilized powder of Saccharomyces cerevisiae prepared in Example 1 and the lyophilized powder of Logger's yeast were mixed according to a mass ratio of 2:1 to obtain component A;
[0070] The lyophilized powder of Lactobacillus rhamnosus prepared in Example 1, the lyophilized powder of Oenococcus oeni, the lyophilized powder of Lactobacillus acidophilus and the lyophilized powder of Lactobacillus brevis were mixed according to the mass ratio of 1:1:1:1 to obtain Component B.
Embodiment 3
[0072] The lyophilized powder of Saccharomyces cerevisiae prepared in Example 1 and the lyophilized powder of Logger's yeast were mixed according to a mass ratio of 4:1 to obtain component A;
[0073] The lyophilized powder of Lactobacillus rhamnosus prepared in Example 1, the lyophilized powder of Oenococcus oeni, the lyophilized powder of Lactobacillus acidophilus and the lyophilized powder of Lactobacillus brevis were mixed according to the mass ratio of 1:2:1:2 to obtain Component B.
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