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Method for detecting squalene in tobacco

A detection method, the technology of the dogfish, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of good reproducibility, high extraction efficiency, and optimized detection conditions

Inactive Publication Date: 2016-06-08
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are currently no qualitative and quantitative detection methods for squalene in tobacco leaves that can be referenced at home and abroad. Therefore, it is very necessary and prospective to establish a qualitative and quantitative detection method for squalene in flue-cured tobacco in the tobacco industry.

Method used

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  • Method for detecting squalene in tobacco
  • Method for detecting squalene in tobacco
  • Method for detecting squalene in tobacco

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Experimental program
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Embodiment 1

[0070] The present embodiment provides a kind of detection method of squalene in tobacco leaf, comprises the following steps:

[0071] S1. Sample pretreatment to obtain the sample solution to be tested;

[0072] S2. preparing a standard solution and establishing a standard curve;

[0073] S3. Detection: draw the sample to be tested and inject it into a high-performance liquid chromatograph for chromatographic separation, and use an ultraviolet absorption detector to detect squalene;

[0074] S4. adopt external standard method, calculate its content according to absorption peak area.

[0075] Wherein, the sample pretreatment method described in step S1 is:

[0076] Sample pretreatment method: take 0.05mL, 10000mg / L squalene standard sample in 10mL n-hexane to obtain external standard base solution, after cutting tobacco leaves, take 5.0g shredded tobacco (take 0.05mL, 10000mg / L squalene standard sample In 10mL of n-hexane, then put 5.0g of shredded tobacco into it, vortex in...

Embodiment 2

[0090] Embodiment 2 Detection method of the present invention and conditional superiority verification test

[0091] For further verifying the superiority of detection method of the present invention and detection condition, carry out following experiment respectively:

[0092] 2.1 Comparison of extraction efficiency of different extractants (C18 column)

[0093] 2.1.1 Sample pretreatment method

[0094] Control group (CK): Take 5.0 g of shredded tobacco (wrapped with filter paper with a diameter of 15 cm) in a Soxhlet extractor, put 200 mL of each extraction solvent into a round-bottomed flask, and put the Soxhlet extractor into a 40°C water bath Reflux for 9 hours, collect the extract, take 30mL from the extract, put it into a separatory funnel, add 30mL of extract methanol-water (V:V=70:30) for extraction, repeat 3 times, collect the extract The solution was evaporated to nearly dryness by rotary evaporation, then dissolved in 5 mL of chromatographic grade acetonitrile, p...

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Abstract

The invention discloses a method for detecting squalene in tobacco. Aiming at a tobacco sample, trichloromethane is taken as an extracting solvent, with combination of targeted Soxhlet extraction, higher extraction efficiency is guaranteed, and HPLC (high performance liquid chromatography) detection is performed after purification treatment with a PSA column. HPLC detection conditions are further optimized, the chromatographic peak is separated better when the method is adopted for detecting the squalene in the tobacco, and the method has the advantages of accuracy, rapidness, high sensitivity, good reproducibility, high recovery rate and the like, and meets qualitative and quantitative requirements.

Description

technical field [0001] The invention relates to the technical field of detection of components in tobacco leaves, and more particularly relates to a method for detecting squalene contained in tobacco leaves based on high performance liquid chromatography. Background technique [0002] Squalene was first discovered from the liver oil of sharks and was named Squalene in 1914, and its chemical name is 2,6,10,15,19,23--hexamethyl--2,6,10,14, 18,22--Tetracosahexaene, which belongs to open-chain triterpenoids. Squalene has various physiological functions such as improving the activity of superoxide dismutase (SOD) in the human body, enhancing the body's antioxidant and immune capabilities, improving sexual function, anti-aging, anti-fatigue, and anti-tumor. It is a non-toxic Biologically active substances that have the functions of preventing and curing diseases. Recent studies have found that squalene also has a detoxifying effect on the human body, removing fat-soluble toxins ...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88G01N2030/027G01N2030/884
Inventor 郑荣豪万树青张功营陈泽鹏覃荣韦建玉
Owner GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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