Method for extracting podocar-pusflavone from juniper berry meal

Abstract

The invention discloses a method for extracting podocar-pusflavone from juniper berry meal. The method comprises the steps that reflux extraction is performed on the juniper berry meal through an ethanol solution with the volume fraction of 70%-80% to obtain an extracting solution, and the extracting solution is concentrated under reduced pressure to obtain concentrate; the concentrate is diluted by adding water, solid-liquid separation is performed after standing is performed, and supernatant liquor is taken; the ethanol degree of the supernatant liquor is regulated to be 20%-30% through ethanol, solid-liquid separation is performed after standing is performed, and precipitates are taken; the precipitates are dissolved with a diluted hydrochloric acid solution and then added into a macroporous resin column, elution is performed with water until the pH value of effluent is neutral, then desorption is performed with the ethanol solution, a desorption solution is collected, and the desorption solution is concentrated under reduced pressure and dried to obtain a juniper berry meal extract. According to the method, the steps are simple and convenient, operation is easy, and the method is suitable for industrialized production; due to the fact that only water and ethanol are adopted as the solvent, not only is the cost greatly reduced, but also no stimulation or toxicity is basically generated to operating personnel and the environment; the content of podocar-pusflavone in the juniper berry meal extract reaches up to 30%-40%, and the extraction efficiency is high.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for extracting bamboo and cypress diflavonoids from juniper pulp. Background technique [0002] Biflavones belong to flavonoids, which are dimers formed by the polymerization of two flavone derivatives, and can be composed of different types of flavone monomer compounds. Natural biflavonoids exist in various vascular plants such as Ginkgo biloba and Selaginella, and new biflavonoids can also be synthesized by chemical methods. With the continuous deepening of research at home and abroad, the cytotoxicity, anti-tumor, anti-virus, anti-bacterial, anti-inflammatory, anti-oxidation and vasodilation of bisflavonoids have attracted more and more people's attention. , health care products and medicine fields also have broad application prospects. [0003] Phyllostachys biflavone is an important class of biflavonoids, and the biflavones obtained from bamboo and cypress i...

AI Technical Summary

Problems solved by technology

[0007] The invention provides a method for extracting biflavonoids from juniper pulp, which solves the problem of low extraction rate of biflavones in the prior art

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