Bionics-based preparation method for tissue-engineered bone

Abstract

The invention discloses a bionics-based preparation method for a tissue-engineered bone. The method comprises the following steps: initiatively preparing bio-derived scaffold materials which are basically similar to natural bone structure and functions and have an allograft source by a 'secondary demineralization technology' according to the principles of 'bionics' and 'autonomous assembly'; selecting bone marrow mesenchymal stem cells (BMMSCs) with a spontaneous osteogenesis phenomenon as seed cells; and simulating a growth microenvironment of in vivo BMMSCs as an inducible factor by a low-oxygen environment to prepare the tissue-engineered bone similar to the natural bone composition, micro-structure and biological reactivity. The tissue-engineered massive bone prepared by the method has obvious bone conductivity and vascularization effect, and can be used for rebuilding and repairing a maxillofacial region or a functional bone with a relatively large bone defect in orthopedics.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a new method of how to prepare tissue-engineered bone based on "bionics" and "self-assembly principle". Background technique [0002] Functional repair and reconstruction of bone defects has always been an important research topic. Bone tissue engineering is an effective means of bone reconstruction / replacement therapy, and the key factors in its research include three aspects: seed cells, scaffold materials, and induction regulators. At present, bone substitute materials can be roughly divided into three categories: autologous bone, artificial substitute materials synthesized from inorganic materials or / and organic materials, and derived bone scaffold materials processed from allogeneic bone. Autologous bone has become the best choice for bone defect repair due to its non-immunogenicity, good osteoinductivity, osteoconductivity and perfect mechanical matching. H...

AI Technical Summary

Problems solved by technology

However, due to the limited source of autologous bone, the small amount of material obtained, and the dysfunction of the donor site, as well as the resulting complications and adverse consequences, it affects their clinical application.

However, artificially synthesized scaffold materials have poor biocompatibility, bioactivity, biodegradability, and mechanical compatibility with host bone.

In addition, it is difficult to imitate the characteristics of natural bone in terms of porosity and pore size; and the acidic degradation products of some materials also affect the attachment, proliferation and differentiation of cells

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