Medicine for treating psoriasis
A technology for psoriasis and drugs, applied in the field of drugs for the treatment of psoriasis, can solve the problems of complex preparation process and high price, and achieve the effects of good safety, improvement of pathological damage, and wide range of social health needs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0020] Embodiment 1: the preparation of the liniment for the treatment of psoriasis
[0021] Drug name: c41 molecule
[0022] The sequence is shown in SEQIDNO: 1, and the modified structure is: T*G*G*C*G*C*G*C*A*C*C*C*A*C*G*G*C*C*T*G , wherein, (* is thio-modified).
[0023] Preparation method: chemical synthesis, entrusted to Shanghai Shenggong Company. Yield, purified by HPLC, vacuumed, stored in glass vials at room temperature, and packaged at 330 μg / vial (dry powder).
[0024] Usage configuration: Take a small bottle, inject 600μl PBS, dilute and mix well, set aside.
Embodiment 2
[0025] .Example 2: Inhibition of activation identification of TLR7 / 8 and 9
[0026] 2.1 Main materials, reagents and equipment:
[0027] 1) Cell line: RAW264.7 (ATCC, USA.).
[0028] 2) Cell culture medium: DMEM (Gibco, Inc., USA).
[0029] 3) Elisa kit: anti-mouse TNF-a (eBioscience, Inc., USA).
[0030] 4) Cell incubator: FORMA3111.
[0031] 5) Microplate reader: full-wavelength multifunctional microplate reader (ThermoScientific, USA).
[0032] 2.2 Experimental method: Dilute RAW264.7 cells to 1×10 6 / mL, added to a 96-well plate (200 μL / well), placed at 37 ° C, 5% CO 2 After culturing in the incubator for 2 hours, the TLR7 / 8 agonist R848 (5 μl, final concentration 100 ng / ml) was added as the experimental group; the TLR9 agonist CpG-ODN18265 μl (5 μl, final concentration 4 μM) was added as the second experimental group. Set 8 multiple wells. Then, each component is divided into 4 multiple wells, and 5 μl of culture solution is added, as a group and two groups of plac...
Embodiment 3
[0034] Example 3: Drugs are applied to treat psoriasis and its curative effect after punching holes with microneedles
[0035] 3.1 Main materials, reagents and equipment:
[0036] 1) Animal: Balb / c (Huafukang Biotechnology Co., Ltd., China).
[0037] 2) Modeling drug: imiquimod (Mingxin Pharmaceutical, China).
[0038] 3) therapeutic drug: liniment of the present invention (see embodiment 1 for details)
[0039] 3.2 Experimental method:
[0040] 1) After the mice were shaved, the psoriasis model was established on the back of the imiquimod, 10mg / cm2, once a day, for 6 days.
[0041] 2) Grouping: placebo group and drug treatment group, each with 5 rats.
[0042] 3) Drug administration channel: use the flywheel microneedle for beauty, and roll it on the skin surface of each mouse.
[0043] 4) Administration: placebo group: smear with phosphate buffered solution (PBS), 200 μl / 20g body weight; treatment group: the C41 drug of the present invention is diluted to 550 μg / ml with...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com