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Mutagenic strains of Cordyceps sinensis producing Cordyceps sinensis mycelium

A technology of Cordyceps sinensis hyphae and Cordyceps sinensis, applied in the field of new strains of Cordyceps sinensis

Inactive Publication Date: 2018-10-09
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The first inventor of this patent, Liu Weimin, once supervised the master student Chen Jing to complete the master's thesis "Research on High-value Cordyceps Liquid Fermentation and Utilization of Rice Bran to Produce Polysaccharides". Liquid fermentation, but further research is needed in terms of efficiency

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  • Mutagenic strains of Cordyceps sinensis producing Cordyceps sinensis mycelium
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  • Mutagenic strains of Cordyceps sinensis producing Cordyceps sinensis mycelium

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Embodiment

[0051] Cordyceps sinensis CCTCC M 2015064 was used as the strain of Cordyceps sinensis. The amount of rice bran used is 3.6g / 100mL medium, the amount of bran used is 4.8g / 100mL medium, and KH 2 PO 4 0.27g / 100mL, MgSO 4 ‘ 7H 2 O 0.27 g / 100mL, cysteine ​​hydrochloride 50mg / 100mL, sodium copper chlorophyllin 10mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use as medium for fermentation, shake bottle The sample volume was 40%, the inoculum volume was 10%, and the culture temperature was 20 o C, rotating speed 180r / min, culture time 5d. The following steps (1) to (7) are carried out: (1) The mycelium containing a small amount of rice bran obtained from liquid culture is separated by centrifugation or centrifugal filtration, washed twice with water, and centrifuged to remove water; ( 2) Freeze-dry the obtained wet mycelium to obtain the finished product of mycelium powder; (3) After filtering and removing slag from the obtained fermented...

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Abstract

The invention discloses a Cordyceps sinensis mutagenic strain for producing Cordyceps sinensis mycelium, and relates to the field of food microbe technology application. The mutated strain has been deposited in the China Center for Type Culture Collection (CCTCC) in Wuhan, China on January 23, 2015, under the number CCTCC M 2015064, and the recommended Latin name is Cordyceps sinensis CCTCC M 2015064. In order to avoid the use of toxic and harmful chemical mutagens and genetic engineering methods that have not been recognized by the public in the food field, considering the simplicity and ease of ultraviolet physical mutagenesis technology, the method of ultraviolet mutagenesis is used for mutagenesis, and through a series of Screening methods such as stability and heredity ensure the excellent properties of the new strain of Cordyceps sinensis, and obtain high-quality industrialized high-efficiency fermentation and low-cost new rice bran bran whole material liquid medium added with cysteine ​​hydrochloride and sodium copper chlorophyllin. Better raw material of Cordyceps sinensis mycelium that can be used to make health food lays the foundation of the strain.

Description

technical field [0001] The invention relates to the application field of food microorganism technology, in particular to a new strain of Cordyceps sinensis obtained by mutagenesis that can be used to ferment liquid rice bran and bran whole material new medium and produce Cordyceps sinensis mycelium raw material. Background technique [0002] Medicinal and edible fungi have a long history of use in China and a wide range of users. Modern science reveals that the mycelium, fruiting bodies or spores of commonly used medicinal and edible fungi such as Cordyceps sinensis, Hericium erinaceus, Ganoderma lucidum, Grifola frondosa, etc. can produce amino acids, proteins, vitamins, polysaccharides, nucleosides, Various active ingredients such as flavonoids and antibiotics, or enriched with various trace elements such as selenium and zinc, have various functions such as improving human immunity, anti-tumor, enhancing liver function, and anti-oxidation. Because of this, the enthusiasm ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/145C12R2001/645
Inventor 刘伟民王雪梅王冲之李婷婷周彬张凤霞狄新园
Owner JIANGSU UNIV
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