A kind of method of producing selenium-enriched Cordyceps sinensis mycelia raw material

A technology of cordyceps sinensis mycelium and selenium enrichment is applied in the field of producing selenium-enriched cordyceps sinensis mycelium raw materials to achieve the effects of protecting public health and reducing production costs

Active Publication Date: 2019-03-05
桐乡市博奥生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The inventor of this patent, Liu Weimin, once guided the master student Chen Jing to complete the master's thesis "Research on High-value Cordyceps Liquid Fermentation and Utilization of Rice Bran to Produce Polysaccharides". , but in-depth research is needed in terms of efficiency

Method used

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  • A kind of method of producing selenium-enriched Cordyceps sinensis mycelia raw material
  • A kind of method of producing selenium-enriched Cordyceps sinensis mycelia raw material

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Experimental program
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Effect test

Embodiment 1

[0071] Cordyceps sinensis CCTCC M 2015064 was used as the strain of Cordyceps sinensis. The amount of rice bran used is 3.6g / 100mL medium, the amount of bran used is 4.8g / 100mL medium, and KH 2 PO 4 0.27g / 100mL, MgSO 4 ·7H 2 O 0.27g / 100mL, cysteine ​​hydrochloride 50mg / 100mL, sodium copper chlorophyllin 10mg / 100mL, sodium selenite 1.33mg / 100mL, natural pH, add water to the required volume, sterilize at 121°C for 30min, Used as a culture medium for fermentation, the sample volume of the shake flask was 40%, the inoculum volume was 10%, and the culture temperature was 20 o C, rotating speed 180r / min, add 1.33mg / 100mL sterilized sodium selenite solution after fermentation 2d, total fermentation time is 5d. The following steps (3) to (10) in the technical solution described in the foregoing summary of the invention are implemented. The results are: mycelium dry weight 4.55 g / 100mL medium, exopolysaccharide 218.5mg / 100mL medium, mycelia polysaccharide 233.4mg / 100mL medium, c...

Embodiment 2

[0073] Cordyceps sinensis CCTCC M 2015064 was used as the strain of Cordyceps sinensis. The amount of rice bran used is 3.0g / 100mL medium, the amount of bran used is 3.0g / 100mL medium, and KH 2 PO 4 0.015g / 100mL, MgSO 4 ·7H 2 O 0.015g / 100mL, cysteine ​​hydrochloride 20mg / 100mL, sodium copper chlorophyllin 5mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use as medium for fermentation, shake bottle The sample volume was 40%, the inoculum volume was 10%, and the culture temperature was 23 o C, rotating speed 180r / min, add 0.21mg / 100mL sterilized sodium selenite solution after 2 days of fermentation, the total fermentation time is 5 days. Culture time 5d. The following steps (3) to (10) in the technical solution described in the foregoing summary of the invention are implemented. The results were: mycelium dry weight 2.95g / 100mL medium, exopolysaccharide 139.8mg / 100mL medium, mycelia polysaccharide 145.6mg / 100mL medium, cordycepin con...

Embodiment 3

[0075] Cordyceps sinensis CCTCC M 2015064 was used as the strain of Cordyceps sinensis. The amount of rice bran used is 3.6g / 100mL medium, the amount of bran used is 3.6g / 100mL medium, and KH 2 PO 4 0.14g / 100mL, MgSO 4 ·7H 2 O 0.14 g / 100mL, cysteine ​​hydrochloride 35mg / 100mL, sodium copper chlorophyllin 7.5mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use as medium for fermentation, shake The sample volume in the bottle is 40%, the inoculum volume is 10%, and the culture temperature is 20 o C, rotating speed 180r / min, add 0.77mg / 100mL sterilized sodium selenite solution after 2 days of fermentation, the total fermentation time is 6 days. The following steps (3) to (10) in the technical solution described in the foregoing summary of the invention are implemented. The results were: mycelium dry weight 3.88g / 100mL medium, exopolysaccharide 186.4mg / 100mL medium, mycelia polysaccharide 196.5mg / 100mL medium, cordycepin content 1.62mg / g...

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Abstract

The invention discloses a method for producing a selenium-rich cordyceps sinensis mycelia raw material and relates to the technical field of food microorganism application. A new cordyceps sinensis strain is used and fermented in a new liquid rice bran and wheat bran complete feed culture medium with selenium, cysteine hydrochloride and sodium copper chlorophyllin added, and the selenium-rich cordyceps sinensis mycelia raw material is produced. Low-value rice bran and wheat bran are converted into the selenium-rich cordyceps sinensis mycelia raw material having multiple healthcare functions and higher quality, and the method has beneficial social and economic effects on reduction of the production cost, efficient and reasonable utilization of low-end resources and protection of public health, so that the method is practical.

Description

technical field [0001] The invention relates to the technical field of food microorganism application, in particular to a method for producing selenium-enriched Cordyceps sinensis mycelia raw material by using a new strain of Cordyceps sinensis obtained by mutagenesis. In the method, the new strain is added with cysteine ​​hydrochloride and copper chlorophyllin. Sodium salt and sodium selenite are fermented in the liquid rice bran bran whole material new medium to obtain the selenium-enriched Cordyceps sinensis mycelia raw material with better quality. Background technique [0002] Medicinal and edible fungi have a long history of use in China and a wide range of users. Modern science reveals that the mycelium, fruiting bodies or spores of commonly used medicinal and edible fungi such as Cordyceps sinensis, Hericium erinaceus, Ganoderma lucidum, Grifola frondosa, etc. can produce amino acids, proteins, vitamins, polysaccharides, nucleosides, Various active ingredients such ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04C12R1/645
CPCC12N1/14C12P19/04
Inventor 朱文静王冲之刘伟民任晓锋王雪梅李婷婷程宇伍娟
Owner 桐乡市博奥生物科技有限公司
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