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Method for producing polymyxin E through fermentation and foam separation coupling

A technology of foam separation and polymyxin, applied in the field of bioengineering, can solve the problems of low total potency of polymyxin E, lower raw material utilization rate, lower enrichment ratio, etc. churn, conversion-boosting effects

Pending Publication Date: 2016-05-04
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] But this method can cause serious inhibitory effect along with the increase of product in fermentation process, makes the total titer of polymyxin E in the fermented liquid lower
For this phenomenon, there are currently existing literatures that use foam separation methods. A bubble distributor is installed at the bottom of the fermenter to separate the target product by bubbling. This method can effectively eliminate product inhibition and increase the yield. Compared with traditional methods Great progress, but in the foam separation process, as the foam phase rises, some nutrients and cells will be taken away, reducing the utilization rate of raw materials, reducing the enrichment ratio, and at the same time due to the separation in the fermenter that acts as a reactor. Large number of air bubbles can affect mass transfer

Method used

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  • Method for producing polymyxin E through fermentation and foam separation coupling
  • Method for producing polymyxin E through fermentation and foam separation coupling
  • Method for producing polymyxin E through fermentation and foam separation coupling

Examples

Experimental program
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Effect test

Embodiment 1

[0047] The Bacillus polymyxa strain obtained from the frozen glycerin tube was inoculated into a slant medium for activation, and cultured at 30°C for 72 hours. The activated bacterial liquid was inoculated into the seed culture medium and cultured at 32°C for 24 hours to obtain the seed liquid (the same in the following examples). The seed liquid was inoculated into a 1.8L fermenter (100) that was sterilized by high temperature at 10% of the inoculum. The fermenter (100) was filled with 1.2L, the culture temperature was 30°C, and the stirring speed was 200r / min. The ventilation volume is 150mL / min. The composition of the fermentation medium is: glucose 20g / L, peptone 10g / L, sodium chloride 1g / L, potassium dihydrogen phosphate 0.1g / L, ammonium sulfate 10g / L, ferrous sulfate 0.1g / L, and ammonia to adjust the initial pH 7,115-121℃ high pressure steam sterilization for 15-30min. After 33 hours of fermentation under these conditions, the detected bacterial content reached 4.19g / L...

Embodiment 2

[0049] The seed liquid was inoculated into a 1.8L fermenter (100) that was sterilized by high temperature at a 10% inoculum. The fermenter (100) had a liquid volume of 1.4L, a culture temperature of 34°C, and a stirring speed of 300r / min. The ventilation volume is 200mL / min. The composition of the fermentation medium is: glucose 20g / L, peptone 10g / L, sodium chloride 1g / L, potassium dihydrogen phosphate 0.1g / L, ammonium sulfate 10g / L, ferrous sulfate 0.1g / L, and ammonia to adjust the initial pH 7,115-121℃ high pressure steam sterilization for 15-30min. After 36 hours of fermentation under these conditions, the detected bacterial content reached 4.01g / L, and the polymyxin E content reached 4712U / mL. At this time, open the discharge valve (4), the feed peristaltic pump (6) and the digital display constant temperature water bath device and constant temperature water bath inlet (306) and constant temperature water bath outlet (307) on the outside of the separation tower to pass the...

Embodiment 3

[0051] The seed liquid was inoculated into a 1.8L fermenter (100) that was sterilized by high temperature at a rate of 10%. The fermenter (100) had a liquid volume of 1.3L, the culture temperature was 32°C, and the stirring speed was 250r / min. The ventilation volume is 200mL / min. The composition of the fermentation medium is: glucose 20g / L, peptone 10g / L, sodium chloride 1g / L, potassium dihydrogen phosphate 0.1g / L, ammonium sulfate 10g / L, ferrous sulfate 0.1g / L, and ammonia to adjust the initial pH 7,115-121℃ high pressure steam sterilization for 15-30min. After 33 hours of fermentation under these conditions, the detected bacterial content reached 4.24g / L and the polymyxin E content reached 4901U / mL. At this time, open the discharge valve (4), the feed peristaltic pump (6) and the digital display constant temperature water bath device and constant temperature water bath inlet (306) and constant temperature water bath outlet (307) on the outside of the separation tower to pass...

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Abstract

The invention provides a method for producing polymyxin E through fermentation and foam separation coupling. According to the method, polymyxin E is prepared by a circulating reflux device formed by a peristaltic pump through fermentation, film separation and foam separation coupling. During coupling operation, fermentation liquor enters a foam separation tower through a film filter by means of the peristaltic pump, products and a liquid pass through the film while cells are intercepted in a fermentation tank, fermentation continues to be performed, and constancy of bacteria is guaranteed; a foam distributor is mounted at the bottom of the foam separation tower, bubbles are produced through air blowing, foam separation is performed, part of nutrients enter the foam separation tower through the film filter, a remaining liquid produced after foam separation returns to the fermentation tank in a circulating reflux manner, and sufficient utilization of the nutrients is guaranteed; coupling outside a tank body is adopted, operation conditions for fermentation and operation conditions for foam separation are controlled respectively, and the best operation conditions can be met.

Description

Technical field [0001] The invention relates to the technical field of bioengineering, in particular to a system and method for producing polymyxin E by coupling fermentation and foam separation. Background technique [0002] Polymyxin E has the characteristics of good antibacterial spectrum, high efficiency, low toxicity, and less residue. It can be used as feed additives to promote the growth of animals and improve feed utilization. It can also prevent the large intestine Escherichia coli that often occurs in feed in large-scale production. For the diseases caused by the contamination of Salmonella and Salmonella, my country has listed polymyxin E as a national legal species, but the product has a low potency and mainly depends on imports. This product is mainly produced by fermentation. The traditional separation method mainly uses ion exchange chromatography, which consumes a lot of energy. This research group uses the foam separation method to separate polymyxin E from the f...

Claims

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Application Information

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IPC IPC(8): C12P21/04C07K7/62C07K1/36C07K1/34C12M1/34C12M1/12C12M1/04C12M1/02C12R1/12
CPCC07K7/62C12M27/02C12M29/00C12M29/02C12M41/26C12M43/00
Inventor 郑辉杰徐丹丹董凯高迎迎
Owner HEBEI UNIV OF TECH
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