A double-stranded polynucleotide-ε-polylysine-sulfated glycan complex with immunomodulatory effect and its preparation and use method
A double-stranded polynucleotide and sulfated polysaccharide technology, applied in the field of medicine, can solve problems such as antibiotic residues, achieve moderate molecular weight, strong resistance to biological enzyme degradation, and improve the effect of immune regulation activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0108] a, 0.1mol / L calcium chloride solution preparation:
[0109]Weigh 1.110 g of anhydrous calcium chloride, dissolve it in 100 ml of water for injection, and filter and sterilize through a filter membrane with a pore size of 0.2 μm to prepare a 0.1 mol / L calcium chloride solution.
[0110] b. Preparation of 0.01mol / L PBS solution (pH=7.6):
[0111] Weigh 0.124g of disodium hydrogen phosphate, 0.018g of sodium dihydrogen phosphate monohydrate, and 0.808g of sodium chloride respectively, dissolve them in 100ml of water for injection after mixing, and filter and sterilize through a filter membrane with a pore size of 0.2μm to make 0.01mol / L PBS solution (pH=7.6).
[0112] c. Preparation of 2mg / ml PolyIC intermediate solution:
[0113] Weigh 1.0088g of PolyI and 0.9912g of PolyC and add 400ml of PBS solution to dissolve, combine the two liquids, mix and shake well, add PBS solution to 1000ml, place in 45°C water bath for 60 minutes, and pass through a filter membrane with a p...
Embodiment 2
[0121] a, 0.1mol / L calcium chloride solution preparation:
[0122] Weigh 1.110 g of anhydrous calcium chloride, dissolve it in 100 ml of water for injection, and filter and sterilize through a filter membrane with a pore size of 0.2 μm to prepare a 0.1 mol / L calcium chloride solution.
[0123] b. Preparation of 0.01mol / L PBS solution (pH=7.6):
[0124] Weigh 0.124g of disodium hydrogen phosphate, 0.018g of sodium dihydrogen phosphate monohydrate, and 0.808g of sodium chloride respectively, dissolve them in 100ml of water for injection after mixing, and filter and sterilize through a filter membrane with a pore size of 0.2μm to make 0.01mol / L PBS solution (pH=7.6).
[0125] c. Preparation of 2mg / ml PolyIC intermediate solution:
[0126] Weigh 1.0088g of PolyI and 0.9912g of PolyC and add 400ml of PBS solution to dissolve, combine the two liquids, mix and shake well, add PBS solution to 1000ml, place in 45°C water bath for 60 minutes, pass through a filter membrane with a pore...
Embodiment 3
[0136] a, 0.1mol / L calcium chloride solution preparation:
[0137] Weigh 1.110 g of anhydrous calcium chloride, dissolve it in 100 ml of water for injection, and filter and sterilize through a filter membrane with a pore size of 0.2 μm to prepare a 0.1 mol / L calcium chloride solution.
[0138] b. Preparation of 0.01mol / L PBS solution (pH=7.6):
[0139] Weigh 0.124g of disodium hydrogen phosphate, 0.018g of sodium dihydrogen phosphate monohydrate, and 0.808g of sodium chloride respectively, dissolve them in 100ml of water for injection after mixing, and filter and sterilize through a filter membrane with a pore size of 0.2μm to make 0.01mol / L PBS solution (pH=7.6).
[0140] c, 2mg / ml preparation of bursal virus RNA solution:
[0141] The bursal virus RNA was dissolved in PBS buffer, and sterilized by filtration through a filter membrane with a pore size of 0.45 μm to prepare a 2 mg / ml solution for later use.
[0142] d. Preparation of 10mg / ml sodium heparin sulfate solution:...
PUM
Property | Measurement | Unit |
---|---|---|
sedimentation rate | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com