Pre-column derivatization device of liquid chromatography and ion chromatography

Abstract

The invention discloses a pre-column derivatization device of a liquid chromatography and an ion chromatography. The pre-column derivatization device comprises a liquid-phase solution, a pump, a reaction device, a sample feeder, a heat-preservation box chromatographic column and a detector, which are connected in sequence, as well as a stirring device, wherein the reaction device comprises a shell, a reaction chamber and a temperature control unit; the reaction chamber is arranged in the shell; one end of the reaction chamber is communicated with the pump and the other end of the reaction chamber is communicated with the sample feeder; a heat-preservation layer is arranged on an outer wall of the reaction chamber; the temperature control unit comprises a temprature control panel and a temperature control circuit board; a groove for storing the temprature control panel is formed in the bottom end face of the reaction chamber; the temprature control panel is fixed in the groove and is used for controlling temperatures of the reaction chamber; the stirring device comprises a motor and fan blades; the motor is arranged outside the reaction chamber and the fan blades are arranged in the reaction chamber; an output shaft of the motor convexly stretches into the reaction chamber and is connected with the fan blades; and the stirring device is used for sufficiently mixing reaction solutions. The pre-column derivatization device provided by the invention has high temperature and reaction time control properties and high derivatization efficiency.

Description

technical field [0001] The invention relates to the technical field of chemical derivatization, in particular to a pre-column derivatization device of liquid chromatography ion chromatography. Background technique [0002] Derivatization is the use of chemical transformations to convert compounds into similar chemical structures. In general, a specific functional compound participates in the derivatization reaction, and the solubility, boiling point, melting point, aggregation state or chemical composition will be deviated. The resulting new chemical properties can be used for quantification or isolation. The role of sample derivatization is mainly to convert difficult-to-analyze substances into substances with similar chemical structures but easy to analyze, which is convenient for quantification and separation. When the detection substance is not easy to be detected, such as no ultraviolet absorption, etc., it can be processed, such as adding a chromophore, etc., to gene...

AI Technical Summary

Problems solved by technology

The disadvantages are: the formed by-products may cause great difficulty in chromatographic separation; during the derivatization process, it is easy to introduce impurities or interference peaks, or cause sample loss

The disadvantages are: additional equipment is required, and the requirements for the instrument are relatively high; the reactor can cause peak broadening and reduce resolution; excessive reagents will cause interference

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