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Preparation method of chicken interferon lyophilized preparation

A technology of chicken interferon and freeze-dried agent, which is applied in the field of preparation of chicken interferon freeze-dried agent, to achieve the effects of improving stability, prolonging half-life, and improving survival rate

Inactive Publication Date: 2016-04-20
宋宏婷
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no effective and practical drug for the treatment of chicken viral diseases, so the development of highly effective antiviral drugs is an important means for the treatment of chicken viral diseases, and it is also an important tool for the prevention and treatment of chicken viral infectious diseases and the development of chicken production. necessary

Method used

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  • Preparation method of chicken interferon lyophilized preparation
  • Preparation method of chicken interferon lyophilized preparation
  • Preparation method of chicken interferon lyophilized preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A preparation method of chicken interferon freeze-dried agent, comprising the following steps,

[0040] Step 1: Aseptically collect the blood of healthy chickens, centrifuge at 10°C for 20 minutes, absorb the white blood cells in the middle layer, and use 0.85% NH 4 After washing twice with Cl, centrifuge to collect the precipitated white blood cells and remove NH 4 Cl, according to the white blood cells: culture medium volume ratio of 1:15, add Eagle`sMEM mixed culture medium containing 5% chicken serum to suspend, and dilute the number of chicken white blood cells to 10×10 7 Individual / ml, make chicken white blood cell suspension;

[0041] Step 2: Take a portion of chicken leukocyte suspension, add chicken leukocyte interferon containing 100 active units, and place it in a 37°C water bath shaker for 36 hours;

[0042] Step 3: Add a certain amount of Newcastle disease virus NDVII to the cell suspension according to the addition amount of 50 HANDVII per milliliter of ...

Embodiment 2

[0047] A preparation method of chicken interferon freeze-dried agent, comprising the following steps,

[0048] Step 1: Aseptically collect the blood of healthy chickens, centrifuge at 10°C for 20 minutes, absorb the white blood cells in the middle layer, and use 0.85% NH 4 After washing twice with Cl, centrifuge to collect the precipitated white blood cells and remove NH 4 Cl, according to the leukocyte: culture medium volume ratio 1:15, add the Eagle`sMEM mixed culture medium containing 5% chicken serum to suspend, and dilute the number of chicken leukocytes to 25×10 7 Individual / ml, make chicken white blood cell suspension;

[0049] Step 2: Take a portion of chicken leukocyte suspension, add chicken leukocyte interferon containing 100 active units, and place it in a 37°C water bath shaker for 36 hours;

[0050] Step 3: Add a certain amount of Newcastle disease virus NDVII to the cell suspension according to the addition amount of 50 HANDVII per milliliter of chicken leukoc...

Embodiment 3

[0055] A preparation method of chicken interferon freeze-dried agent, comprising the following steps,

[0056] Step 1: Aseptically collect the blood of healthy chickens, centrifuge at 10°C for 20 minutes, absorb the white blood cells in the middle layer, and use 0.85% NH 4 After washing twice with Cl, centrifuge to collect the precipitated white blood cells and remove NH 4 Cl, according to the leukocyte: culture medium volume ratio 1:15, add the Eagle`sMEM mixed culture medium containing 5% chicken serum to suspend, and dilute the number of chicken leukocytes to 20×10 7 Between each / ml, the chicken leukocyte suspension was prepared;

[0057] Step 2: Take a portion of chicken leukocyte suspension, add chicken leukocyte interferon containing 100 active units, and place it in a 37°C water bath shaker for 36 hours;

[0058] Step 3: Add a certain amount of Newcastle disease virus NDVII to the cell suspension according to the addition amount of 50 HANDVII per milliliter of chicken...

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Abstract

The invention discloses a preparation method of chicken interferon lyophilized preparation and belongs to the field of animal vaccines. The preparation method of the chicken interferon lyophilized preparation comprises six steps as follows: healthy chicken blood is collected sterilely and is subjected to centrifugation at the temperature of 10 DEG C for 20 min, stratum intermedium leukocytes are sucked, washed twice with NH4Cl with the mass percentage of 0.85% and then subjected to centrifugation, precipitated leukocytes are collected, NH4Cl is removed, an Eagle's MEM mixed culture solution containing 5% of chicken serum is added in a volume ratio of the leukocytes to the culture solution being 1:15 for suspension, the chicken leukocytes are diluted to be in a range from 10*10<7> / ml to 25*10<7> / ml, and a chicken leukocyte suspension is prepared. The preparation method of the chicken interferon lyophilized preparation has the characteristics that the long-acting chicken virus interferon preparation is prepared, chicken are injected with the preparation every 10 days, and the preparation has good effect on chicken virus diseases.

Description

technical field [0001] The invention relates to a preparation method of a freeze-dried agent, in particular to a preparation method of a chicken interferon freeze-dried agent. Background technique [0002] Chicken interferon is one of the earliest animal interferons studied, and chicken interferon was reported in the 1980s. The chicken interferons that have been discovered include INF-α, β, ω, δ and INF-γ, and now the genes have been cloned, sequenced and mapped. In recent years, the induction conditions and physical and chemical activities of chicken interferon have been further studied, and a large number of clinical trials have been carried out on chicken leukocyte interferon. Studies have shown that it has a significant effect on many viral infectious diseases of chickens, such as epidemic diarrhea, chicken plague, infectious gastroenteritis, etc., as well as cattle viral diarrhea, gosling plague, lamb diarrhea, etc. Experiments have confirmed that chicken interferon T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K47/48A61K47/16A61K47/42A61K38/21C07K14/555C07K1/107A61P31/12A61K47/60
CPCA61K9/19A61K9/0019A61K38/21A61K47/16A61K47/42C07K14/555
Inventor 宋宏婷
Owner 宋宏婷
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