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Sample pre-treatment method for measuring the quantity of toxic elements in agricultural produce samples

A crop and element technology, applied in the preparation of test samples, test food, measuring devices, etc., can solve the problems of insufficiently ensuring the mobility of cadmium ions, frequent failure of grinders, and low extraction of cadmium, etc. The effect of short time, low measurement cost, and high measurement accuracy

Inactive Publication Date: 2016-03-30
SUMIKA CHEM ANALYSIS SERVICE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the above-mentioned method, this method can be measured in a short time, but when the sample is grains, beans or seeds, it needs to be finely pulverized with a mill (millser) during pretreatment, In the measurement of multiple samples, it takes a long time to wash the grinder and finely pulverize the grinder, so there are problems such as frequent grinder failures.
In addition, in this method, cadmium is extracted by increasing the contact rate with hydrochloric acid by finely pulverizing the sample, but the mobility of cadmium ions from the plant cell structure cannot be sufficiently ensured, so there is a problem that the amount of cadmium extracted often occurs. The problem with significantly fewer specimens

Method used

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  • Sample pre-treatment method for measuring the quantity of toxic elements in agricultural produce samples
  • Sample pre-treatment method for measuring the quantity of toxic elements in agricultural produce samples
  • Sample pre-treatment method for measuring the quantity of toxic elements in agricultural produce samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] After roughly pulverizing 50 brown rice samples with a near-infrared moisture meter (manufactured by Ketuto Scientific Research Institute, Rice Wheat Moisture Meter SP-1D3 type), the method of the present invention was prepared according to the following extraction operation (pretreatment method of the present invention) of the extract.

[0043] Extract operation:

[0044] (i) Put 1 g of a brown rice sample roughly pulverized with a moisture meter into a PP bottle (polypropylene bottle), and then add 2.5 mL of ion-exchanged water (see figure 1 (i)).

[0045] (ii) Put the PP bottle in a microwave oven without the cap on it, heat it at 500W for about 30 seconds, add 2.5 mL of an enzyme solution (an equal volume mixed solution of amylase, protease, and cellulase: 10% by weight) and cover it Put the cap on the PP bottle, and shake it up and down gently for about 10 seconds ( figure 1 (ii) cf.).

[0046] (iii) Take off the cover of the PP bottle, add 5mL of 0.2N hydrochl...

Embodiment 2

[0061] 20 soybean samples were beaten with an agate mortar to coarsely pulverize them, and then the extract solution of the present invention was prepared according to the pretreatment method of the present invention in the same manner as in Example 1.

[0062] On the other hand, the conventional method A extract was produced in the same manner as in Example 1 in accordance with the aforementioned conventional method A from 20 similar soybean samples.

[0063] Next, the amount of each element of cadmium, manganese, and zinc was measured using ICP-OES (VISTA-MPX manufactured by SII) for the extract prepared by the pretreatment method of the present invention and the extract prepared by the conventional method A, and used in atomic The amount of total arsenic was measured by a device with a hydride generator attached to an absorptiometer (atomic absorptiometer: AA-7000 manufactured by Shimadzu Corporation, hydride generator: HVG-1 manufactured by Shimadzu Corporation) . Then, t...

Embodiment 3

[0073] 20 sesame samples were beaten with an agate mortar to coarsely pulverize them, and then the extract solution of the present invention was prepared in the same manner as in Example 1 according to the pretreatment method of the present invention.

[0074] On the other hand, from 20 identical sesame samples, according to the conventional method A, the extract liquid of the conventional method A was produced similarly to Example 1.

[0075] Next, the amount of each element of cadmium, manganese, and zinc was measured using ICP-OES (VISTA-MPX manufactured by SII) for the extract prepared by the pretreatment method of the present invention and the extract prepared by the conventional method A, and used in atomic The amount of total arsenic was measured by a device with a hydride generator attached to an absorptiometer (atomic absorptiometer: AA-7000 manufactured by Shimadzu Corporation, hydride generator: HVG-1 manufactured by Shimadzu Corporation) . Then, the amount of the ...

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Abstract

Provided is a method with which it is possible to simply, accurately, rapidly and at a high extraction rate carry out on-site the pre-treatment of samples when measuring the quantity of toxic elements in agricultural produce samples of cereals, beans or seeds. This is a pre-treatment method for samples for measuring of the quantity of at least one element selected from a group consisting of cadmium, arsenic, zinc, manganese, copper, lead and chromium, in agricultural produce samples selected from cereals, beans and seeds, characterized by containing: (i) a step in which the sample is coarsely ground; (ii) a step in which water is added to the coarsely ground sample and heated, transforming the [beta] starch contained in the sample into [alpha] starch; (iii) a step in which an enzyme is added to the sample, transforming the [alpha] starch in the sample into sugar; (iv) a step in which hydrochloric acid is added to the sample to extract the elements being measured in the sample; and (v) a step in which solids are removed from the extracted liquid. The rough grinding of the sample is preferably carried out when measuring the moisture content of the sample, and the heating of the roughly-ground sample is preferably carried out by microwave.

Description

technical field [0001] The present invention relates to a kind of pretreatment method of the sample that is used for measuring the amount of harmful elements such as cadmium in the crop samples such as rice, soybean, sesame etc. in the spot, relates to a kind of method compared with the past, especially the time of operation is shortened. It is a method to reduce the time and effort and improve the extraction rate of the measured elements. Background technique [0002] In recent years, the accumulation of environmental pollutants in various scenes of industry and life has become a problem in view of the improvement of society's environmental awareness and health impact on environmental conservation. Among environmental pollutants, harmful elements such as cadmium have been causing serious problems due to their toxicity, and it is very important to know the amount of harmful elements contained in crops. [0003] The amount of harmful elements contained in crops is usually me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/10G01N33/10
CPCG01N33/10G01N2001/4061
Inventor 俵田启中村胜雄
Owner SUMIKA CHEM ANALYSIS SERVICE
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