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Method for nondestructively testing trepang autolysis on basis of nuclear magnetic resonance technique

A nuclear magnetic resonance and non-destructive testing technology, which is applied in the direction of nuclear magnetic resonance analysis, material analysis through resonance, and measuring devices, to achieve the effects of promoting seafood quality control, short time consumption, and high accuracy

Inactive Publication Date: 2016-03-23
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the application of low-field NMR technology to the study of sea cucumber autolysis

Method used

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  • Method for nondestructively testing trepang autolysis on basis of nuclear magnetic resonance technique
  • Method for nondestructively testing trepang autolysis on basis of nuclear magnetic resonance technique
  • Method for nondestructively testing trepang autolysis on basis of nuclear magnetic resonance technique

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The market buys five fresh sea cucumbers (parallel samples), and the quality is 45-50g. Pour fresh sea cucumbers into a clean 500mL beaker, take out one sea cucumber, and use a paper knife to cut a gap of about 3 cm longitudinally in the abdomen of the sea cucumber, pull out the intestines of the sea cucumber from the opening, and use scissors to open the abdominal cavity of the sea cucumber. Several gray tendons attached to the inner wall are cut off to obtain sea cucumbers with intestines dissected.

[0031] Naturally placed, NMR signal acquisition at 0, 1, 2, 3, 4, 6, 10, 36, 48 hours:

[0032] Adopt MiniMR-Rat magnetic resonance imaging analyzer, utilize Carr-Purcell-Meiboom-Gill (CPMG) sequence, measure sea cucumber sample transverse relaxation time T2, parameter setting is: 90 degree pulse width P1: 13 μ s, 180 degree pulse width P2: 26μs, repeat sampling waiting time Tw: 5000ms, analog gain RG1: 15, digital gain DRG1: 3, preamplifier gain PRG: 1, NS: 8, NECH: 80...

Embodiment 2

[0034] Example 2: Monitoring the Autolysis Process of Fresh Sea Cucumber by Magnetic Resonance Weighted Imaging

[0035] A fresh sea cucumber sample, 48.82g, was selected and dissected according to the method of Example 1, put into a 90cm glass petri dish, and placed at room temperature for 48h. Using MiniMR-Rat magnetic resonance imaging analyzer, using multi-layer spin echo (SE) sequence, measuring the transverse relaxation time T2 and magnetic resonance imaging (T1 weighted imaging and T2 weighted imaging) of fresh sea cucumber samples during autolysis, parameter settings For: slices: 5, FovRead: 100mm, FovPhase: 100mm, slicewidth: 3.0mm, slicegap: 2.0mm, Readsize: 256, Phasesize: 192, Average: 4, T1 weighted imaging: TR: 300, TE: 19; T2 weighted Imaging: TR:1600, TE:50. To establish a method for detecting sea cucumber autolysis by nuclear magnetic resonance. After magnetic resonance T1-weighted imaging and T2-weighted imaging ( Figure 4 , the original picture is in col...

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Abstract

The invention provides a method for nondestructively testing trepang autolysis on basis of a nuclear magnetic resonance technique. The method comprises the following steps: carrying out nuclear magnetic resonance signal collection on the trepang under the same parameters by adopting a magnetic resonance imaging analyzer and utilizing a CMPG sequence, thereby acquiring a transverse relaxation spectrum; and then judging the change of the content of free water in the trepang according to the degree of left shifting of T2 relaxation peak. According to the method for analyzing the water content change in the trepang autolysis process through low-field nuclear magnetic resonance provided by the invention, the transverse relaxation time spectrum T2 and the magnetic resonance imaging of the fresh bowel-cut trepang are taken as main analyzing basis, so as to quickly judge the water content change in the trepang autolysis process. The method provided by the invention has the advantages that the accuracy of the measuring result is high, the consumed time is short, the sample is free from being damaged, the inner visible information of the sample can be supplied, and the like. The reliable information is supplied to the detection for the autolysis process of the bowel-cut trepang and the change in the quality during the autolysis process of the bowel-cut trepang.

Description

technical field [0001] The invention belongs to the field of analysis and measurement of materials, and in particular relates to a qualitative analysis method based on nuclear magnetic resonance. Background technique [0002] Sea cucumber (SeaCucumber), a kind of echinoderm, also known as sea rat and sea melon, has a slightly cylindrical body and soft texture. There are many types, common ones are sea cucumber, black ginseng, plum ginseng and so on. Nutritious, rich in protein, low in fat and cholesterol. In addition, it also contains sea cucumber mucopolysaccharide and sea cucumber, 8 kinds of essential amino acids that cannot be synthesized by the human body, among which arginine and lysine are the most abundant. Studies have shown that sea cucumbers contain autolytic enzymes, which have a strong autolytic ability, especially after being irradiated by ultraviolet rays, the autolysis is more obvious, and the original normal life activities are disordered, and vomiting and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08
CPCG01N24/082
Inventor 谭明乾李永宋亮程沙沙周婉茹
Owner DALIAN POLYTECHNIC UNIVERSITY
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