Gold nano catalyst with stable yeast cells and preparation method and application thereof
A technology of yeast cells and catalysts, which is applied in the preparation of amino compounds, chemical instruments and methods, metal/metal oxide/metal hydroxide catalysts, etc. Preparation time, high catalytic activity, and the effect of enhancing adsorption performance
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Embodiment 1
[0062] Yeast cell culture and treatment: As described above, yeast cells were cultured in shake flasks, and then the yeast were collected and washed.
[0063] Add Tween-80 with a mass percentage concentration of 0.1-5%, shake at 40-60°C at 100r / min for 24-48 hours, centrifuge, wash, and freeze-dry to obtain the modified yeast cell carrier .
[0064] Preparation of gold nanocatalysts stable in yeast cells: Add 5.0mL2mmol / L of chloroauric acid solution and 5.0mL1mol / L sodium hydroxide solution into 100mg of chemically treated modified yeast cell carrier, mix thoroughly, and place at 37 In an air bath shaker at ℃, react in the dark at 100r / min for 24h, centrifuge at 3000r / min for 10min, wash with twice distilled water for 3 times, and freeze-dry.
Embodiment 2
[0066] The preparation steps of cultivating and treating yeast and yeast cell-stabilized gold nanocatalyst are the same as in Example 1, except that the added chemical reagent is TritonX-100 with a concentration of 0.1-5% by mass.
Embodiment 3
[0068] The preparation steps of cultivating and treating yeast and the stable gold nanocatalyst of yeast cells are the same as in Example 1, except that the added chemical reagent is cetyltrimethylammonium bromide with a concentration of 0.1-5% by mass.
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