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Magnetic polysaccharide nanometer gel material preparation method

A nanogel, polysaccharide technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problem of low magnetic flux of polysaccharide nano or microsphere materials, difficult to achieve targeted delivery of drugs, difficult to meet clinical use, etc. problems, to achieve the effect of eliminating toxic residues, good positioning effect, and large drug loading

Inactive Publication Date: 2016-02-17
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] (1) Chemical reagents such as glutaraldehyde or divinyl sulfone are used for cross-linking. This type of chemical cross-linking agent is cytotoxic. Although it can make polysaccharide microspheres or gel materials relatively stable in structure, the toxicity remains It is easy to cause the denaturation of cell growth factors, which is difficult to meet the clinical use
[0010] (2) The prepared polysaccharide nano or microsphere materials have small magnetic flux and poor positioning effect, making it difficult to achieve the purpose of targeted drug delivery
[0011] (3) The drug loading is small and the sustained release performance is poor, especially the particle size of the microspheres is too large and can only release the drug in the blood vessels of the target area, resulting in poor application effect in the human body

Method used

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  • Magnetic polysaccharide nanometer gel material preparation method
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  • Magnetic polysaccharide nanometer gel material preparation method

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preparation example Construction

[0043] The method for preparing magnetic polysaccharide nanogel provided by the present invention has specific steps including:

[0044] Step 1. Prepare an aqueous solution of heparin with a mass / volume concentration of 0.6-2% at room temperature, and add dropwise a thymine hydrochloric acid solution with a mass concentration of 0.1-0.2%, the volume of which is 1 / 5 to 1 / 2 of the heparin solution. Stir the reaction at ℃~80℃ for 12~24 hours, adjust the reaction solution to neutral with ammonia water, then extract with ethyl acetate, and evaporate to dryness to obtain the basicized heparin;

[0045] Step 2. Prepare a chitosan aqueous solution with a mass / volume concentration of 0.3-1% at room temperature, and add dropwise adenine hydrochloric acid solution with a mass concentration of 0.1-0.2%, whose volume is 1 / 10-1 / of the chitosan solution 5. Stir the reaction at 50°C to 80°C for 12 to 24 hours, adjust the reaction solution to neutral with ammonia water, then extract with ethyl ace...

Embodiment 1

[0050] (1) Prepare a 2% heparin aqueous solution with a mass / volume concentration at room temperature, add dropwise a 0.2% mass / volume concentration of thymidine hydrochloric acid solution, whose volume is 1 / 2 of the heparin solution, and stir for 24 hours at 50°C. , Adjust the reaction solution to neutral with ammonia water, then extract with ethyl acetate, and evaporate to dryness to obtain alkalinized heparin. The preparation diagram is shown in figure 1 ;

[0051] (2) Prepare chitosan aqueous solution with a mass / volume concentration of 0.5% at room temperature, and add dropwise adenine hydrochloric acid solution with a mass / volume concentration of 0.2%, whose volume is 1 / 5 of the chitosan solution, at 50°C The reaction was stirred for 24 hours, the reaction solution was adjusted to neutral with ammonia water, and then extracted with ethyl acetate and evaporated to dryness to obtain alkalized chitosan. The preparation diagram is shown in figure 1 ;

[0052] (3) Dissolve ferrous...

Embodiment 2

[0056] (1) Prepare a 0.6% heparin aqueous solution with a mass / volume concentration of 0.6% at room temperature, add dropwise a 0.1% mass / volume concentration of thymine hydrochloric acid solution whose volume is 1 / 5 of the heparin solution, and stir for 12 hours at 60°C. , Adjust the reaction solution to neutral with ammonia water, then extract with ethyl acetate, and evaporate to dryness to obtain basic heparin;

[0057] (2) Prepare an aqueous solution of chitosan with a mass / volume concentration of 0.3% at room temperature, and add dropwise adenine hydrochloric acid solution with a mass / volume concentration of 0.1%, whose volume is 1 / 6 of the chitosan solution, at 60°C The reaction was stirred for 12 hours, the reaction solution was adjusted to neutral with ammonia water, and then extracted with ethyl acetate and evaporated to dryness to obtain alkalized chitosan;

[0058] (3) Dissolve ferrous chloride and ferric chloride in water at room temperature, their molar mass ratio is 1...

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Abstract

The invention discloses a magnetic polysaccharide nanometer gel material and a preparation method thereof, and provides a targeting controlled release means for a cell growth factor based on magnetic force effect. According to the invention, magnetic ferriferrous oxide nano particles are embedded in polysaccharide nano gel, base pairing crosslinking of the nano gel can be realized, and the magnetic polysaccharide nanometer gel material with a stable structure can be obtained. In the invention, the cell growth factor enables targeting controlled release by applying magnetic force externally, and usage of a chemical cross-linking agent can be avoided, biological activity of the embedded medicine can be guaranteed, and material usage security can be obviously improved. The preparation method has the advantages of simple process, easy operation, low temperature, fast solidification speed and short processing period, and has the application value on medicine controlled release, tissue engineering and regenerative medicine.

Description

Technical field [0001] The invention belongs to the field of biomedical polymer materials, and specifically relates to a preparation method of a magnetic polysaccharide nanogel material. Background technique [0002] Natural polysaccharides have excellent biocompatibility and adjustable biodegradability, and can be used for the embedding and release of drugs, proteins or genes. They have been widely used in the fields of controlled drug release, tissue engineering and regenerative medicine. Commonly used natural polysaccharides include: hyaluronic acid, alginic acid, chondroitin sulfate, chitosan, heparin and cellulose. [0003] In the field of tissue engineering and regenerative medicine, polysaccharide microgels and nanogels are often used to embed cell growth factors and genes to improve the biological activity of the cell scaffold and increase the tissue inducing ability of the scaffold in vivo. The use of appropriate controlled release means can achieve the targeted delivery ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61K47/02A61K9/06B82Y5/00C08B37/10C08B37/08C08J3/09
Inventor 谈花平范茗闫敬萱马烨丘加财李先明
Owner NANJING UNIV OF SCI & TECH
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