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Stable good-interference resistance serum zinc detection reagent and detection method

A technology for detection reagents and detection methods, which is applied in the field of serum zinc detection, can solve the problems of expensive instruments and difficult popularization in primary hospitals, and achieve the effects of avoiding pretreatment steps, improving sensitivity and stability, and improving stability

Inactive Publication Date: 2016-01-13
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Atomic absorption spectrophotometry has high specificity and is easy to operate, but the instrument is very expensive and difficult to popularize in primary hospitals

Method used

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  • Stable good-interference resistance serum zinc detection reagent and detection method
  • Stable good-interference resistance serum zinc detection reagent and detection method
  • Stable good-interference resistance serum zinc detection reagent and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Detection reagents for serum zinc, including reagent R1 and reagent R2:

[0054] 1) The composition of its R1 is:

[0055] Tris (trishydroxymethylaminomethane) buffer (pH=8.2, 25°C) ·························· 100mmol / L

[0056] Salicylaldoxime ·································································································· 5mmol / L,

[0057] dimethylglyoxime salt ······························································································· 2mmol / L,

[0058] Fluorocarbon Surfactant FSO-300 ··········································································· 10ml / L,

[0059] 2,9-Dimethyl-1,10-phenanthroline ··········································································· 3mmol / L,

[0060] ascorbic acid ······································································································ 5mmol / L,

[0061] Potassium ferrocyanide ··································································...

Embodiment 2

[0084] Interference test: Take fresh mixed serum, divide it into 2 equal parts, then divide each equal part into 7 equal parts, add different interfering substances, so that the concentration in the serum reaches Figure 4 requirements. Then respectively use the reagent obtained in embodiment 1, and the serum zinc (Zn) reagent that is common and approved in the market to compare and measure the content of Zn in the serum simultaneously, the measured results of the control group and the measured results of each group after adding different interfering substances are shown in Figure 4 . Relative deviation (%) = (measuring mean value of interference samples - measuring mean value of control samples) / measured mean value of control samples × 100%.

[0085] Depend on Figure 4 It can be seen that the reagent of Example 1 has a triglyceride≤1000mg / dL, bilirubin≤50mg / dL, Cu 2+ ≤60μmol / L, Fe 3+ ≤60μmol / L, Ca 2+ ≤5mmol / L, Mg 2+ ≤4mmol / L has no obvious interference to the test res...

Embodiment 3

[0087] Correlation experiment: using the formula of Example 1 to prepare the reagents, compared with the serum zinc kit of a company approved by the State Food and Drug Administration, which is common in the market, and tested 20 clinical serum samples at the same time. The test results are as follows: Figure 5 shown. And obtained the correlation curve of the two reagents (such as figure 1 Shown), the test results show that the correlation coefficient of the two kits is 0.9994, indicating that there is a great correlation between the two.

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Abstract

The invention relates to the technical field of serum zinc detection and especially relates to a serum zinc detection reagent. A reagent R1 contains a buffer, salicylaldoxime, dimethylglyoximate, a fluorocarbon surfactant FSO-300, 2, 9-dimethyl-1, 10-phenanthroline, ascorbic acid, potassium ferrocyanide, polyethylene glycol 6000, glycol, trisodium citrate, NaCl and an antiseptic. A reagent R2 contains a buffer, dimethyl sulfoxide, nitro-PAPS, a fluorocarbon surfactant FSO-300, ascorbic acid, potassium ferrocyanide, polyethylene glycol 6000, glycol, trisodium citrate, NaCl and an antiseptic. The Tris buffer, the stabilizing agent and a plurality of anti-interference agents are used, can effectively eliminate interference caused by copper, iron, calcium and magnesium ions and can substantially improve reagent stability. The used novel non-ionic fluorocarbon surfactant FSO-300 can promote serum zinc cracking, prevent reaction system turbidity, improve chromogenic substance stability, prevent a pretreatment process such as protein removal and substantially improve reagent sensitivity and stability.

Description

technical field [0001] The invention relates to the technical field of serum zinc detection, in particular to a serum zinc detection reagent and a detection method using the detection reagent. Background technique [0002] Zinc is one of the trace elements necessary for human to maintain life. It is absorbed by intestinal mucosal cells in the intestinal tract and combined with albumin into the blood. Zinc is mainly excreted in feces. The important physiological function of zinc is to maintain the cofactor of various enzymes in the human body. There are more than 90 kinds of metalloenzymes that must have zinc to perform their normal functions, such as carbonic anhydrase and alkaline phosphatase in red blood cells, and synthesis of DNA and RNA. related enzymes, etc. Zinc plays an important role in maintaining the structure of enzymes. When combined with membrane proteins, it can stabilize the membrane structure and prevent membrane lipid peroxidation. [0003] Zinc is very ...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/78
Inventor 李志明李静谭柏清
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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