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Preparation method of biomimetic bone material containing graphene, hydroxyapatite and fibroin

A technology of hydroxyapatite and biomimetic materials, applied in medical science, prostheses, etc., can solve problems such as lack of biological activity, poor hydrophilicity, inflammation, etc., achieve good cell affinity, good biocompatibility, and improve Effects of Compressive Strength and Toughness

Inactive Publication Date: 2015-12-23
ZHONGYUAN ENGINEERING COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, inorganic materials are brittle and their degradation performance is difficult to control; organic polymer materials have insufficient mechanical properties and poor hydrophilicity, and their acidic degradation products are easy to cause inflammation and lack biological activity combined with human tissues; and the safety performance of nanomaterials still to be studied

Method used

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  • Preparation method of biomimetic bone material containing graphene, hydroxyapatite and fibroin
  • Preparation method of biomimetic bone material containing graphene, hydroxyapatite and fibroin

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Embodiment 1

[0025] A preparation method of a nano-bone biomimetic material containing graphene, hydroxyapatite and silk fibroin, comprising the following steps:

[0026] (1) Ultrasonic dispersion of graphene oxide into 0.5 mol / L sodium hydroxide ethanol (85%) solution with a bath ratio of 1:500, alkalized at 30°C for 60 min. Then add 0.725mol / L sodium chloroacetate solution, ultrasonically stir at 60°C for 50min, adjust the pH value of the solution to neutral with 0.1mol / L hydrochloric acid and dialyze in distilled water for three days to obtain carboxylated graphene aqueous solution;

[0027] (2) According to the calcium-phosphorus molar ratio of 1.67, 0.05mol / L Ca(OH) 2 with diluted H 3 PO 4 Use a burette to drop the solution into the solution prepared in step (1) at the same time and stir in ultrasonic for 4 hours, add ammonia water drop by drop to control the reaction pH to 8.0-9.0, and control the reaction temperature to 60°C to obtain graphene / hydroxyapatite Composite particle su...

Embodiment 2

[0033] A preparation method of a nano-bone biomimetic material containing graphene, hydroxyapatite and silk fibroin, comprising the following steps:

[0034] (1) Ultrasonic dispersion of graphene oxide into 1mol / L sodium hydroxide ethanol (85%) solution with a bath ratio of 1:500, alkalized at 50°C for 20min. Then add 1.25mol / L sodium chloroacetate solution, ultrasonically stir for 100min at 50°C, adjust the pH value of the solution to neutral with 0.3mol / L hydrochloric acid and dialyze in distilled water for three days to obtain carboxylated graphene aqueous solution;

[0035] (2) According to the calcium-phosphorus molar ratio of 1.67, 0.1mol / L Ca(OH) 2 with diluted H 3 PO 4 Use a burette to drop the solution into the solution prepared in step (1) at the same time and stir in ultrasonic for 2 hours, add ammonia water drop by drop to control the reaction pH to 8.0-10.0, and control the reaction temperature to 75°C to obtain graphene / hydroxyapatite Composite particle suspen...

Embodiment 3

[0040] A preparation method of a nano-bone biomimetic material containing graphene, hydroxyapatite and silk fibroin, comprising the following steps:

[0041] (1) Ultrasonically disperse graphene oxide into 0.08mol / L sodium hydroxide ethanol (85%) solution with a bath ratio of 1:500, and basify at 40°C for 30min. Then add 0.1mol / L sodium chloroacetate solution, ultrasonically stir at 55°C for 60min, adjust the pH value of the solution to neutral with 0.2mol / L hydrochloric acid and dialyze in distilled water for three days to obtain carboxylated graphene aqueous solution;

[0042] (2) According to the calcium-phosphorus molar ratio of 1.67, 0.03mol / L Ca(OH) 2 with diluted H 3 PO 4 Use a burette to drop the solution into the solution prepared in step (1) at the same time and stir in ultrasonic for 3 hours, add ammonia water drop by drop to control the reaction pH to 8.0-10.0, and control the reaction temperature to 80°C to obtain graphene / hydroxyapatite Composite particle susp...

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Abstract

The invention relates to a biomimetic bone material containing graphene / hydroxyapatite / fibroin and a preparation method of the biomimetic bone material. The biomimetic bone material is prepared by assembling hydroxyapatite which is obtained through coprecipitation on the surface of carboxylation modified graphene through electrostatic adsorption, then blending the graphene assembled with the hydroxyapatite with fibroin solutions different in mass concentration, and freeze-drying layer by layer sequentially from higher mass concentration to lower mass concentration so as to obtain the biomimetic bone material. The graphene / hydroxyapatite / fibroin biomimetic bone material prepared by the invention simulates natural bone in ingredient and structure; the biomimetic bone material is excellent in biocompatibility and biodegradability, and is outstanding in mechanical property and porosity of graded distribution; and the biomimetic bone material, which can be used as a repair or substitute material of bone tissues, is broad in application prospect.

Description

technical field [0001] The invention relates to a nano-bone biomimetic material containing graphene / hydroxyapatite / silk fibroin and a preparation method thereof, which is applied to bone repair or bone replacement materials, and specifically relates to the technical field of biomedical materials. Background technique [0002] In the field of orthopedics, bone defects caused by severe traffic accidents, tumors, osteomyelitis and other reasons are very common. The traditional method uses autologous bone or allogeneic bone transplantation for treatment. The source of autologous bone is limited and complications may occur in the bone extraction area, and bone extraction may cause trauma to the second operation area. Allogeneic bone has more sources than autologous bone, but there is immune rejection between it and the recipient tissue, and there is a possibility of infection. Therefore, the key to solving bone defect repair materials is to develop an artificial bone material th...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/12A61L27/08A61L27/50A61L27/56
Inventor 何建新谭卫琳韩啟明杨勇连艳平王利丹崔世忠丁彬
Owner ZHONGYUAN ENGINEERING COLLEGE
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