Bactericide compounded by eupatorium adenophorum extract and mefenoxam
A technology for the compounding of fenaxyl and Eupatorium adenophorum, which is applied in the direction of fungicides, biocides, biocides, etc., can solve the problems of not being very clear, functional research, and not easy to degrade, etc., to increase the use effect, The effect of fast degradation and reduced usage
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Embodiment 1
[0015] Example 1 Extraction of Eupatorium adenophorum Extract
[0016] The collected leaves of Eupatorium adenophorum were dried in the shade and pulverized into a coarse powder, adding methanol 6 times the weight of the coarse powder, ultrasonically extracting at room temperature three times, 12 hours each time, combining the filtrates, concentrating under reduced pressure at 45°C to obtain an extract. Add a small amount of methanol to the extract to dissolve, add distilled water to dilute the methanol content to 20%, extract with ethyl acetate, recover the solvent under reduced pressure at 45°C from the extract to dryness, and obtain the ethyl acetate extract. The ethyl acetate extract was dissolved in a mixed solution of methanol:water:chloroform (85:15:5), and eluted on an XAD-2 macroporous resin column to remove impurities such as pigments, and the eluent was collected and concentrated to obtain a paste. The decolorized paste was subjected to repeated silica gel column ...
Embodiment 2
[0017] Embodiment 2 antibacterial experiment
[0018] For the test bacteria: Phytophthora capsici, Pythium ingross.
[0019] Drugs to be tested: mefenaxyl (Syngenta Company), and zelenone were prepared by the method in Example 1.
[0020] The extract of Eupatorium adenophorum and the combined fungicide containing the extract of Eupatorium adenophorum were tested for in vitro biological activity of the tested pathogenic bacteria by the method of mycelial growth rate. Calculate the bacteriostatic rate by following formula: bacterial colony growth diameter (mm)=two times measure diameter average value-4.0 (bacteria cake diameter); Inhibition growth rate=(diameter of control bacterium colony-diameter of processing bacterium colony) / diameter * 100 of control bacterium colony %.
[0021] According to the formula of Colby's method (1966) to calculate the control effect and compare with the measured control effect, it is simple and effective to evaluate the combined effect of the ...
Embodiment 3
[0030] Embodiment 3 is to the control field experiment of capsicum phytophthora
[0031] Pharmaceutical composition 1 was prepared. Composition 1 contained 62.5 mg / L metalaxyl, 250 mg / L zelenone, 50 mg / L xylene and 50 mg / L dimethyl sulfoxide, and the rest was water.
[0032] Prepare pharmaceutical composition 2, which contains 62.5 mg / L metalaxyl, 250 mg / L zelenone, 50 mg / L xylene and 50 mg / L polyoxyethylene sorbitan fatty acid ester, and the rest is water.
[0033] Take 200m of pepper field for planting 2 , Infected with Phytophthora capsici and developed disease, moderately infected, divided into two parts on average, applied the pesticides of pharmaceutical composition 1 and pharmaceutical composition 2, sprayed 1L each time, sprayed once every other day, sprayed 3 times, and applied the pesticide after 15 days The pepper recovery rate of composition 1 was 95%, and the pepper recovery rate of agent composition 2 was 99%.
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