Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel application of sulfated bletilla striata polysaccharide and preparation for treating ocular surface damage

A Bletilla striata polysaccharide and sulfation technology, applied in the field of medicine, can solve the problems of poor viability of hUC-MSCs and unsatisfactory corneal epithelial repair effect, and achieve the effect of repairing corneal damage, promoting migration and promoting proliferation

Inactive Publication Date: 2015-12-16
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing research results show that the survival ability of hUC-MSCs in vitro is poor, and the effect of directly using them for corneal epithelial repair is not ideal.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel application of sulfated bletilla striata polysaccharide and preparation for treating ocular surface damage
  • Novel application of sulfated bletilla striata polysaccharide and preparation for treating ocular surface damage
  • Novel application of sulfated bletilla striata polysaccharide and preparation for treating ocular surface damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Bletilla striata decoction pieces were ground into powder, dispersed and dissolved in distilled water at 80°C for 4 hours, and then filtered to remove impurities.

[0053]The filtrate was soaked overnight in 3 volumes of 95% ethanol, and the precipitate was resuspended with distilled water.

[0054] Add 1 / 3 volume of a mixture of chloroform and butanol (volume ratio 5:1.) Mix evenly and centrifuge to extract protein components, and extract the liquid in the aqueous phase layer.

[0055] Dialyzed through a 30000D-50000D dialysis column (the dialysate is distilled water), made into freeze-dried powder, and obtained the crude product of Bletilla striata polysaccharide. The crude Bletilla striata polysaccharide was dissolved and further purified by cross-linked dextran G-100 to obtain the Bletilla striata polysaccharide.

[0056] Sulfation modification of Bletilla striata polysaccharide by chlorosulfonic acid-pyridine method. The specific steps are:

[0057] Dissolve 0.2...

Embodiment 2

[0059] Rinse the umbilical cord twice in PBS containing 100 U / mL penicillin and 100 U / mL streptomycin, add pre-cooled 75% alcohol, soak 1-2min, during which the umbilical cord was kept turning; PBS was added to rinse twice to wash away the alcohol. Cut the umbilical cord into small sections of about 2 mm with tissue scissors, and cut each section longitudinally with ophthalmic scissors, remove three blood vessels (two arteries and one vein) in the umbilical cord with vascular forceps, and remove the adventitia of the umbilical cord at the same time; Cut it into tissue pieces with a size of about 1mm3, take an appropriate amount and put it into a sterile petri dish with a diameter of 10cm, covering 70% of the bottom area of ​​the petri dish. Add LONZA human stem cell serum-free medium (LonzaUltraCULTURE TM ), 5% CO 2 , 37°C, 95% humidity in CO 2 cultured in an incubator. On the 5th to 7th day, half of the medium was changed, and the culture was continued for about 12 to 14 ...

Embodiment 3

[0066] The sulfated Bletilla striata polysaccharide prepared in Example 1 was dissolved in PBS to prepare a 50 μg / ml-400 μg / ml polysaccharide solution. Experimental study on the biocompatibility of polysaccharide solutions with different concentrations and human corneal epithelial cells. The method is: take a 6-well plate, label it for 24h, 48h, and 72h respectively, and prepare the suspension of human corneal epithelial cells (purchased from Shanghai Cell Bank, Chinese Academy of Sciences) at 1×10 4 Cells / well were planted, and on the second day of culture, sulfated Bletilla striata polysaccharide solutions with different concentrations (0, 50 μg / ml, 100 μg / ml, 200 μg / ml, 300 μg / ml, 400 μg / ml) were added to each well, and the cells were separated in 24 hours, 100 μg / ml, and 400 μg / ml. After 48 hours and 72 hours, the MTT method was used to detect the changes of cell proliferation in each group and the results of statistical analysis were shown in Table 2:

[0067] Table 2 Bi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Quality scoreaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of medicines, particularly relates to a novel application of sulfated bletilla striata polysaccharide and a preparation for treating ocular surface damage, and provides an application of sulfated bletilla striata polysaccharide in preparation of a preparation for treating ocular surface damage, a preparation containing sulfated bletilla striata polysaccharide and umbilical cord mesenchymal stem cells and an application of the preparation in treatment of ocular surface damage. Tests prove that the preparation compounded by the umbilical cord mesenchymal stem cells and the sulfated bletilla striata polysaccharide can be used for remarkably promoting reproduction of corneal epithelial cells, and has an effect remarkably superior to independent application of the sulfated bletilla striata polysaccharide or umbilical cord mesenchymal stem cells. Cell migration tests indicate that the preparation and bletilla striata polysaccharide can be used for remarkably promoting migration of the corneal epithelial cells, but the effect for using the preparation is remarkably superior to independent application of the sulfated bletilla striata polysaccharide or umbilical cord mesenchymal stem cells. Therefore, the preparation has an effect of repairing corneal injury.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the new application of sulfated bletilla striata polysaccharide and a preparation for treating ocular surface damage. Background technique [0002] Severe ocular surface injury is the most common and most difficult disease to treat in ophthalmology. accounted for more than 60%. At present, allogeneic corneal transplantation and artificial corneal transplantation are mostly used in clinic to treat ocular surface injury. However, allogeneic corneal transplantation faces problems such as transplant rejection and insufficient donors; and the foreign body property of artificial corneal construction materials does not support the growth of corneal epithelial cells, and its transparency, plasticity and stretching force are still limiting its development. important factor. [0003] For this reason, a large number of clinical studies in recent years have begun to try to use seed cells combined ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/737A61K35/28A61P27/02
Inventor 葛啸虎陈海佳王一飞戚康艺马岩岩王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com