Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit and test strip for detecting sulfonamides and use thereof

A kit and sulfonamide technology, applied in the field of immunoassay, can solve problems such as low sensitivity, unsuitable for rapid detection, difficulty in obtaining antibodies, etc.

Inactive Publication Date: 2015-12-09
CHINA AGRI UNIV
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the detection methods of sulfa drugs mainly include instrumental methods (including high-performance gas chromatography, high-performance liquid chromatography, and chromatography-mass spectrometry), microbial methods, immunoassay methods, and receptor analysis methods. These methods have their own characteristics: instrumental methods Accurate, reliable, and highly sensitive, it is a confirmatory method, but it is not suitable for rapid on-site detection; microbiological methods can detect multiple antibiotics at the same time, but the sensitivity is not high, the detection time is long, and it cannot be quantified; the immunoassay method is based on the specificity of antigens and antibodies It has the advantages of high sensitivity, strong specificity, and quantitative detection. However, due to high sensitivity and the difficulty of obtaining antibodies that can recognize the same class of drugs, its application in the field of veterinary drug residue detection is restricted.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit and test strip for detecting sulfonamides and use thereof
  • Kit and test strip for detecting sulfonamides and use thereof
  • Kit and test strip for detecting sulfonamides and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Embodiment 1, the preparation of protein flop

[0087] 1. Preparation of protein flops

[0088]Replace the sequence between the NdeI and XhoI recognition sites of the expression vector pET-28b(+) with the nucleotide sequence shown in SEQ ID NO.2, keep the other sequences of the expression vector pET-28b(+) unchanged to obtain a recombinant expression vector pET-28b(+)-flop. It is proved by DNA sequencing that the flop gene in the recombinant expression vector pET-28b(+)-flop is the nucleotide sequence shown in SEQ ID NO.2, which encodes the protein flop shown in SEQ ID NO.1.

[0089] The recombinant expression vector pET-28b(+)-flop was transformed into Escherichia coli BL21(DE3) competent cells to obtain a recombinant strain containing the DNA sequence for encoding the protein flop shown in SEQIDNo.2, and the strain was named recombinant Escherichia coli BL21-pET28b-flop-7 (hereinafter referred to as BL21-pET28b-flop-7 strain).

[0090] Recombinant Escherichia coli ...

Embodiment 2

[0101] Embodiment 2, the test kit that detects sulfa drug

[0102] The configuration of each reagent in this embodiment is as follows:

[0103] Preparation of PBS buffer solution with a concentration of 0.05M and a pH value of 7.0: Solution A: 17.9gNa 2 HPO 4 · 12H2O, 1L deionized water, to get a concentration of 0.05M Na 2 HPO 4 Solution; Solution B: 8.6gKH 2 P0 4 2H 2 O, 1 L of deionized water to obtain KH at a concentration of 0.05M 2 P0 4 Solution; take 60mL of solution A and 40mL of solution B, add 8.5g of NaCl, and mix well.

[0104] The preparation of PBS buffer solution with a concentration of 0.01M and a pH value of 7.4: 8gNaCl, 0.2gKCl, 3.53gNa 2 HPO 4 12H 2 O, 0.24gKH 2 PO 4 , 1L deionized water.

[0105] Preparation of PBS buffer solution with a concentration of 0.1M and a pH value of 7.2: Solution C: 35.8gNa 2 HPO 4 12H 2 O, 1 L deionized water to get a concentration of 0.1 M Na 2 HPO 4 solution; solution D: 15.6gNaH 2 P0 4 2H 2 O, 1L deioniz...

Embodiment 3

[0179] Embodiment 3, detect the colloidal gold test strip of sulfa drug

[0180] The configuration of each reagent in this embodiment is as follows:

[0181] The preparation of sodium carbonate solution with a concentration of 0.05M and a pH value of 9.6: 1.59gNa 2 CO 3 , 2.93g NaHCO 3 , add water to make up to 1L.

[0182] The preparation of PBS buffer solution with a concentration of 0.01M and a pH value of 7.4: 8gNaCl, 0.2gKCl, 3.53gNa 2 HPO 4 12H 2 O, 0.24gKH 2 PO 4 , 1L deionized water.

[0183] Preparation of PBS buffer solution with a concentration of 0.05M and a pH value of 7.0: Solution A: 17.9gNa 2 HPO 4 12H 2 O, 1L deionized water; solution B: 8.6gKH 2 P0 4 2H 2 O, 1L of deionized water; take 60mL of solution A and 40mL of solution B, add 8.5g of NaCl, and mix well.

[0184] 1. Preparation of colloidal gold test strips

[0185] 1. Preparation of coating agent

[0186] The hapten 4-(4-amino-benzenesulfonamido)benzoic acid (CS) was coupled to ovalbumi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit and a test strip for detecting sulfonamides and a use thereof. The kit and colloidal gold test strip prepared from a protein flop expressed by a preserved bacterial strain Escherichia coli BL21(DE3)-pET28b-flop are used for detecting sulfonamides. The kit and colloidal gold test strip have the advantages of high sensitivity, high accuracy, low cost, operation simpleness and long shelf-life, realize fast detection of a lot of samples, realizes on-site high flux rapid detection and produces a great effect in sulfonamide detection.

Description

technical field [0001] The invention relates to a test kit, a test strip and an application thereof for detecting sulfa drugs in the field of immunoanalysis. Background technique [0002] Sulfa drugs are a class of chemical drugs with the structure of sulfanilamide. Among them, dozens of drugs are widely used and have definite curative effects. Because of their low price and convenient use, they are widely used in animal disease prevention and growth promotion. The irrational use of sulfa drugs has caused serious residues in livestock products. The sulfa drugs residues in food will endanger people's health. Long-term intake of food containing sulfa drug residues can increase bacterial resistance and cause kidney toxicity. , Affect the hematopoietic system and other toxic side effects. [0003] As people pay more and more attention to food safety, the European Union, China, the United States, Japan and other countries have successively made regulations on the maximum residue...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68
CPCG01N33/558G01N33/68
Inventor 沈建忠王战辉梁晓张素霞温凯宋晓丽江海洋
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com