Method for continuously preparing lactic acid bacterial liquid for fermented feed

Abstract

The invention discloses a method for continuously preparing lactic acid bacterial liquid for fermented feed, and belongs to the field of animal fermented feed. The method comprises the steps that feedstuff is adopted as main raw materials, stirred evenly, then sterilized and contained in a abacterial fermenting vessel; 5% of the lactic acid bacterial liquid and 95% of abacterial water are added according to the weight of solid-state matrixes, mixed evenly and cultivated at 35 DEG C for 24 h, and then the mixture is washed with 18-20 times 0.5% abacterial glucose solutions containing 200 U / g acid proteases of protein feedstuff and 400 U / g amylases of starch feedstuff; effluent liquid is collected into a cultivating tank with a stirring function to be cultivated at 37 DEG C at 30 r / min for 2-4 h, and the number of lactic acid bacteria can be more than 109 / mL. The solid-state fermentation matrixes are washed once every 16-24 h and totally can be washed for 8 times repeatedly. The method can supply the technical basis to production of lactic acid bacteria seeds of the fermented feed industry such as fermented soybean meal, fermented peanut meal, fermented cotton meal and fermented repeseed meal and is suitable for large-area application and popularization.

Description

technical field [0001] The invention relates to a method for continuously preparing lactic acid bacteria liquid for fermented feed, belonging to the field of animal fermented feed. Background technique [0002] At present, an important factor affecting the development speed and quality of my country's aquaculture industry is the shortage of feed raw materials. The main raw materials of the feed industry are grain raw materials (accounting for about 60%), protein raw materials (accounting for about 30%) and additives. Due to the fact that our country has a lot of people and little land, the unbalanced structure of grain, economy and feed crops has not been effectively resolved for a long time. The supply of raw materials has become the bottleneck of the development of the feed industry, especially the shortage of protein feed raw materials. It is and will be in the future. The most important factor restricting the development of my country's feed industry. [0003] At presen...

AI Technical Summary

Problems solved by technology

However, due to the high production cost of fermented feed, according to incomplete statistics, there is currently only about 500,000 tons of fermented protein feed, which is still a big gap compared with the approximately 50 million tons of protein feed raw materials in the feed industry.

[0006] At present, the lactic acid bacteria liquid used for feed fermentation is prepared by step-by-step expansion cultivation of more than three levels, which has a long life cycle (about 72 hours) and a large investment in equipment (for example, feeding fermented feed every day, it needs to be equipped with 3 sets of seed expansion cultivation equipment) and High cost of culture (synthetic medium, multiple steam sterilization) and other deficiencies