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Probiotics preparation for preventing and treating diabetic encephalopathy and method for preparing probiotics preparation

A technology of diabetic encephalopathy and bacterial strains, applied to the new use of butyric acid bacteria, the application field of butyric acid bacteria in the preparation of compositions for the prevention and treatment of diabetic encephalopathy, can solve the lack of understanding of diabetic encephalopathy and intestinal microecological structure , There are no reports on the research and application of butyric acid bacteria, etc., to achieve the effect of convenient use, improvement of intestinal flora imbalance, and improvement of learning and memory ability

Inactive Publication Date: 2015-12-02
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Butyric acid bacteria generally exist in the intestines of soil, animals and humans. They are Gram-positive anaerobic bacteria that can tolerate gastric acid entering the intestinal tract and secrete butyric acid (butyric acid), an important nutrient for intestinal mucosal regeneration and repair. Injured intestinal mucosa can eliminate inflammation and nourish the intestinal tract. It has a good curative effect on intestinal diseases caused by various reasons including intestinal bacterial infection, tumor chemotherapy, surgery, etc. People are concerned about diabetic encephalopathy and intestinal microecological structure. Insufficient understanding, scientific workers do not have much research on this aspect, so far, there is no report on the research and application of butyric acid bacteria on the prevention and treatment of diabetic encephalopathy

Method used

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  • Probiotics preparation for preventing and treating diabetic encephalopathy and method for preparing probiotics preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1 Isolation and identification of Clostridium butyricum WZ001 strain

[0028] Taking the feces of a healthy young man as an isolated sample, on the modified MRS agar medium, under anaerobic conditions at 37° C., 48h coating isolation and culture to obtain the butyric acid bacteria (Clostridium butyricum) WZ001 of the present invention, identified as D. Acidobacteria.

[0029] The formula of the modified MRS medium: peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, K 2 HPO 4 2g, MgSO 4 ·7H 2 O0.5g, MnSO 4 .4H 2 O0.2g, fructooligosaccharide 3g, diammonium citrate 2g, agar 15g, distilled water 1L, adjust pH to 7.0, sterilize at 115°C for 15 minutes.

[0030] Clostridiumbutyricum WZ001 of the present invention has the following microbiological characteristics:

[0031] (1) Colony morphology: milky white, round and slightly convex, with irregular edges, 1-3 mm in diameter, slightly glossy surface, and bubbles in the liquid medium.

[0032] (2) Ind...

Embodiment 2

[0036] Example 2 Preparation of Clostridiumbutyricum WZ001 probiotic preparation composition

[0037] 1. Seed liquid preparation: Pick a single colony of Clostridium butyricum WZ001, inoculate it in 50 mL of modified MRS liquid medium, pH 7.0 ± 0.5, 37 ° C, anaerobic culture for 24 hours, that is, the seed liquid;

[0038] The formula of modified MRS medium: peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, sodium acetate 5g, K 2 HPO 4 2g, MgSO 4 ·7H 2 O0.5g, MnSO 4 ·4H 2 O0.2g, fructooligosaccharide 3g, diammonium citrate 2g, distilled water 1L, adjust pH to 7.0, sterilize at 115°C for 15min.

[0039] 2. Fermentation culture: take the seed liquid of step 1, inoculate it into a new improved MRS medium with 1% inoculum, and culture it anaerobic for 24 hours at 37°C, and finish the culture;

[0040] 3. Collect the bacteria: take the fermentation broth in step 2, remove the fermentation supernatant at 12,000 rpm for 10 minutes, and separate the bacteria by centr...

Embodiment 3

[0043] Example 3 Animal Experimental Research on Prevention and Treatment of Diabetic Encephalopathy

[0044] Objective: To observe the effect of the butyric acid bacillus preparation of the present invention on learning and memory, intracerebral oxidative indexes and morphological changes of nerve cells in diabetic encephalopathy mice.

[0045] 1. Materials and methods:

[0046] 1. Drug: Clostridium butyricum WZ001 powder, the number of viable bacteria is 1×10 8 CFU / g, see Example 2 for the specific preparation method.

[0047] 2. Experimental animals: male ICR mice, weighing 18-22 g, male, provided by the experimental animal center of the school.

[0048] 2.1 Preparation of the diabetic encephalopathy mouse model: The mice were fasted for 12 hours for a single intraperitoneal injection of streptozotocin 50 mg / kg, and the blood glucose level of the animals every other week was ≥11.1 mmol / L. These diabetic mice were further operated on. Using permanent right unilateral car...

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Abstract

The invention belongs to the field of life science, relates to application of probiotics preparations to preparing medicine for preventing and treating diabetic encephalopathy, particularly relates to novel application of probiotics clostridium butylicum for medicine for preventing and treating diabetic encephalopathy, and discloses a probiotics preparation for preventing and treating diabetic encephalopathy. The diabetic encephalopathy refers to chronic comprehensive mental functional disorder, long-term diabetes patients progressively suffer from the chronic comprehensive mental functional disorder, and cognitive dysfunction is a main clinical feature of the chronic comprehensive mental functional disorder. The probiotics preparation has the advantages that the probiotics preparation is free of optional toxic and side effects; intestinal flora are creatively used as treatment target points, novel modes for preventing and treating the diabetic encephalopathy are created, accordingly, disease symptoms and nerve cell injury can be relieved, and the probiotics preparation has excellent social and economical benefits and is worthy of being popularized and applied; clostridium butylicum is a main ingredient of the probiotics preparation, conventional excipients are added into the probiotics preparation, and accordingly the probiotics preparation can be in dosage forms of tablets, capsules, granules, powder, oral liquid, enema and the like which are suitable to be used clinically.

Description

technical field [0001] The invention relates to a new use of butyric acid bacteria, in particular to the application of butyric acid bacteria in the preparation of a composition for preventing and treating diabetic encephalopathy, and belongs to the field of biopharmaceuticals. Background technique [0002] Diabetes has high morbidity, many complications, high mortality and high disability rate. According to the World Health Organization (WHO) report in 2008, there were as many as 384 million diabetic patients worldwide, and the incidence rate in my country was about 9.5%, conservatively estimated to be at least 80 million. Most of them are (90-95)% type 2 diabetes. Diabetes has many diseases and complications, such as coronary heart disease, hypertension, myocardial infarction, stroke, senile dementia, Parkinson's disease, nephropathy, retinopathy, etc. The incidence of cardiovascular and cerebrovascular complications is more than 3 times higher than that of non-diabetic p...

Claims

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Application Information

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IPC IPC(8): A61K35/741A23L1/30A23L2/38A61P3/10A61P25/00
Inventor 孙晶虞希冲王方岩林燕郑园园刘佳明
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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