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Novel direct bilirubin detection kit

A detection kit and bilirubin technology, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by causing chemical reaction of materials, etc., can solve the problem that the anti-interference ability is not very strong, and the price of reagents is difficult to promote. , high sensitivity of oxidase method, to achieve the effect of high clinical value, good reagent stability and good repeatability

Inactive Publication Date: 2015-11-25
郁东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The diazonium method has the longest history and is widely used so far. However, the method is affected by many factors when determining direct bilirubin, and the results are quite different. High performance liquid chromatography (HPLC) has requirements for instruments and equipment. high, so it cannot be used as a conventional method; the oxidase method has high sensitivity, but the anti-interference ability is not very strong, mainly because the enzyme has extremely high temperature requirements, and its activity can only play its best role in a certain temperature range, and this kind of The price of method reagents is extremely high and it is difficult to promote

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] A novel direct bilirubin detection kit is composed of reagent R1 and reagent R2 in a volume ratio of 4:1;

[0067] The reagent R1 is composed of the following substances:

[0068] Tris-HCl tartrate sodium hydroxide buffer (pH5.0-6.0) 0.05mol / L

[0069] Glycerol 0.015mol / L

[0070] Dithiothreitol 0.005mol / L

[0071] Silver nitrate 0.008mol / L

[0072] Methyl isobutyl ketone 0.2%

[0073] Triton X-1000.15%

[0074] Na 2 -EDTA0.01g / L

[0075] Hydroxylamine hydrochloride 0.8g / L;

[0076] Described reagent R2 is made up of the substance of following content:

[0077] Disodium hydrogen phosphate 1.0g / L

[0078] Sodium dihydrogen phosphate 3.0g / L

[0079] Sodium persulfate 0.060mol / L

[0080] Na 2 -EDTA0.7g / L

[0081] NaCl0.05%.

Embodiment 2

[0083] A novel direct bilirubin detection kit is composed of reagent R1 and reagent R2 in a volume ratio of 4:1;

[0084] The reagent R1 is composed of the following substances:

[0085] Tris-HCl tartrate sodium hydroxide buffer (pH5.0-6.0) 0.1mol / L

[0086] Glycerol 0.01mol / L

[0087] Dithiothreitol 0.01mol / L

[0088] Silver nitrate 0.005mol / L

[0089] Methyl isobutyl ketone 0.5%

[0090] Triton X-1000.1%

[0091] Na 2 -EDTA0.05g / L

[0092] Hydroxylamine hydrochloride 0.4g / L;

[0093] Described reagent R2 is made up of the substance of following content:

[0094] Disodium hydrogen phosphate 1.5g / L

[0095] Sodium dihydrogen phosphate 2.5g / L

[0096] Sodium persulfate 0.074mol / L

[0097] Na 2 -EDTA0.5g / L

[0098] NaCl0.1%.

Embodiment 3

[0100] A novel direct bilirubin detection kit is composed of reagent R1 and reagent R2 in a volume ratio of 4:1;

[0101] The reagent R1 is composed of the following substances:

[0102] Tris-HCl tartrate sodium hydroxide buffer (pH5.0-6.0) 0.15mol / L

[0103] Glycerol 0.005mol / L

[0104] Dithiothreitol 0.015mol / L

[0105] Silver nitrate 0.002mol / L

[0106] Methyl isobutyl ketone 0.8%

[0107] Triton X-1000.05%

[0108] Na 2 -EDTA0.1g / L

[0109] Hydroxylamine hydrochloride 0.1g / L;

[0110] Described reagent R2 is made up of the substance of following content:

[0111] Disodium hydrogen phosphate 2.0g / L

[0112] Sodium dihydrogen phosphate 1.5g / L

[0113] Sodium persulfate 0.085mol / L

[0114] Na 2 -EDTA0.3g / L

[0115] NaCl0.15%.

[0116] In the present invention: it is carried out on the automatic operation instrument Mindray 800; the sample sample (the Landau quality control product is used as the sample sample, and the calibration product is used as the calibrati...

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Abstract

The invention discloses a novel direct bilirubin detection kit. The novel direct bilirubin detection kit consists of a reagent R1 and a reagent R2, wherein the reagent R1 consists of a Tris-HCl tartaric acid sodium hydroxide buffer solution, glycerine, dithiothreitol, silver nitrate, methyl isobutyl ketone, triton X-100, Na2-EDTA and oxammonium hydrochloride; the reagent R2 consists of sodium hydrogen phosphate, sodium biphosphate, sodium persulfate, Na2-EDTA and NaCl. All the components are scientifically combined, the accuracy in measuring a sample within 0-400 [mu]mol / L is relatively good, the precision is high, the repeatability is good, the anti-interference ability is strong, the reagents are relatively good in stability, and the novel direct bilirubin detection kit is suitable for a full-automatic biochemical analyzer and has a high clinical use value.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a novel direct bilirubin detection kit. Background technique [0002] Bilirubin contains two types: free bilirubin and direct bilirubin: direct bilirubin can also be called conjugated bilirubin, that is, free bilirubin in the blood is transported to the liver and is quickly taken up by liver cells. In the cytoplasm of liver cells, it is mainly combined with Y protein and Z protein (mainly Y protein). Then combine with glucuronic acid in the endoplasmic reticulum to generate water-soluble conjugated bilirubin; free bilirubin can also become unconjugated bilirubin, that is, not combined with glucuronic acid, unconjugated bilirubin is mainly heme in Under the catalysis of mononuclear phagocytic system cell microsomal heme oxygenase, the water-soluble biliverdin of linear tetrapyrrole is formed, and the biliverdin is further reduced under the catalysis o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/79
Inventor 郁东李建营谭柏清甘宜梧谢清华
Owner 郁东
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