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Method for remediation of estradiol in soil on basis of immobilized microbial technology

A technology of immobilized microorganisms and microbial technology, which is applied in the field of soil restoration, can solve the problems of limiting effects, and achieve the effects of wide application, good air permeability and high activity

Inactive Publication Date: 2015-11-11
SHENYANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of estradiol mainly focuses on the removal in the water environment. Common treatment methods include adsorption, chemical oxidation, photocatalytic degradation, and biodegradation. As well as the complexity and heterogeneity of the soil environment, the application of treatment methods in the water environment to soil is limited and the effect is poor

Method used

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  • Method for remediation of estradiol in soil on basis of immobilized microbial technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. A method for repairing estradiol in soil based on immobilized microorganism technology

[0026] (1) Pretreatment of immobilized carrier material

[0027] The fresh cotton cake and pine needles are air-dried at room temperature and then mixed at a weight ratio of 1:1, crushed or crushed through a 40-mesh standard sieve for use. Pyrophyllite particles with a particle size of 0.2-1 mm are combined with the sieved cotton kernels The cake and the pine needles are mixed evenly according to the weight ratio of 4:1:1, then put into the sterilization pot, sterilized by high-temperature steam at 121°C for 2.5 hours, put into the plastic bucket and built in the sterile room until the temperature drops to room temperature, for use;

[0028] (2) Preparation of immobilized microbial particles

[0029] The bacterial suspension containing estradiol-degrading bacteria is evenly added to the surface of the pretreated immobilized carrier and mixed evenly. The inoculum is 3.5% of the ca...

Embodiment 2

[0036] Example 2: Degradation effect of immobilized microbial particles on estradiol

[0037] (1) Pretreatment of immobilized carrier material

[0038] The fresh cotton cake and pine needles are air-dried at room temperature and then mixed at a weight ratio of 1:1, crushed or crushed through a 40-mesh standard sieve for use. Pyrophyllite particles with a particle size of 0.2-1 mm are combined with the sieved cotton kernels The cake and the pine needles are mixed evenly according to the weight ratio of 4:1:1, then put into the sterilization pot, sterilized by high-temperature steam at 121°C for 2.5 hours, put into the plastic bucket and built in the sterile room until the temperature drops to room temperature, for use;

[0039] (2) Preparation of immobilized microbial particles

[0040] The bacterial suspension containing estradiol-degrading bacteria is evenly added to the surface of the pretreated immobilized carrier and mixed evenly. The inoculation amount is 3% of the carrier weight...

Embodiment 3

[0045] Example 3 Degradation effect of immobilized microbial particles on estradiol

[0046] (1) Pretreatment of immobilized carrier material

[0047] The fresh cotton cake and pine needles are air-dried at room temperature and then mixed at a weight ratio of 1:1, crushed or crushed through a 40-mesh standard sieve for use. Pyrophyllite particles with a particle size of 0.2-1 mm are combined with the sieved cotton kernels The cake and the pine needles are mixed evenly according to the weight ratio of 4:1:1, then put into the sterilization pot, sterilized by high-temperature steam at 121°C for 2.5 hours, put into the plastic bucket and built in the sterile room until the temperature drops to room temperature, for use;

[0048] (2) Preparation of immobilized microbial particles

[0049] The bacterial suspension containing estradiol-degrading bacteria is evenly added to the surface of the pretreated immobilized carrier and mixed evenly. The inoculum is 4% of the carrier weight, and it is...

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Abstract

The invention relates to a method for remediation of estradiol in soil on basis of the immobilized microbial technology, relates to a method for soil remediation. The method comprises the following steps: mixing pyrophyllite, decorticated cottonseed meal and pine needle according to the weight part ratio of pyrophyllite to decorticated cottonseed meal to pine needle being 4:1:1 to obtain a microbial immobilized carrier material, sterilizing, inoculating a bacterium suspension which accounts for 2-5% of the total weight of the carrier, additionally adding an enrichment medium according to the dosage of 1.5 ml of enrichment medium for 1 g of the carrier each day to obtain immobilized microbial granules, and determining a proper feed ratio of the prepared immobilized microbial granules according to the weight of soil, so as to be used for remediation of estradiol contaminated soil. According to the method for remediation of estradiol in soil on basis of the immobilized microbial technology, provided by the invention, estradiol in soil is degraded by utilizing the immobilized microbial technology, and the degradation effect of estradiol under different environment temperatures, pH values and soil moisture contents and other soil environment conditions can be examined.

Description

Technical field [0001] The invention relates to a method for repairing soil, in particular to a method for repairing estradiol in soil based on an immobilized microorganism technology. Background technique [0002] Environmental Estrogens (EnvironmentalEstrogens) are a kind of biological organisms by interfering with the synthesis, secretion, transport, binding, active reaction, metabolism, digestion or production of similar organisms' own hormones to maintain the normal dynamic balance and reproduction of organisms. Environmental chemical substances (exogenous biological substances) that adversely affect growth and behavior, also known as Endocrine Disrupting Chemicals (EDCs). [0003] Human and animal excrement are the main source of estradiol in the natural environment. In order to promote the growth of livestock and poultry and shorten the breeding cycle, some farms add a large amount of growth hormones to the feed. These hormones will be discharged into the environment along ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/02B09C1/10C12R1/07C12R1/38
Inventor 杨悦锁宋晓明高翠萍王晨彭彤彤
Owner SHENYANG UNIV
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