Hantavirus-like particles containing gm-csf, preparation method and application thereof

Abstract

The invention relates to a hantaan virus-like particle containing GM-CSF as well as a preparation method and application of hantaan virus-like particles. For the VLP (virus-like particle) composed of multiple proteins, baculoviruses for expressing all proteins are required to be prepared on the basis of an insect cell expression system, and then insect cells are co-infected according to different proportions, so that a lot of work for exploring a co-infection proportion is required, and in addition, a certain difficulty is brought to purification in a later period due to baculoviruses mixed in the product. According to the invention, on the basis of a mammalian cell expression system, by constructing a eukaryotic expression vector to co-transfect dhfr-CHO cells, and the hantaan virus-like particle containing GM-CSF is assembled. According to the invention, all components of the chimeric virus like particle can express, the constructed eukaryotic expression vector contains multiple selection markers, a cell strain for stably expressing chimeric virus like particles can be constructed through screening, mass production and preparation are facilitated and the hantaan virus-like particle can be used for preventing hemorrhagic fever with renal syndrome, which is caused by hantaan virus infection.

Description

technical field [0001] The invention relates to a vaccine, in particular to a hantavirus-like particle containing GM-CSF and its preparation method and application. Background technique [0002] Hemorrhagic fever with renal syndrome (HFRS) is an acute viral infectious disease caused by Hantavirus. At present, there is no specific and effective treatment drug for this disease, and it mainly relies on vaccines for prevention. Although inactivated virus vaccines for hemorrhagic fever with renal syndrome have been developed in China, such vaccines stimulate the body to produce low neutralizing antibody titers and cannot effectively stimulate the body to produce cellular immune responses. In addition, primary cell and brain tissue inactivated vaccines Most of the raw materials come from animals, and the quality control of animal feeding, especially the possible harm of potential animal viruses to humans, is a key issue that needs to be resolved. With the development of biotechn...

AI Technical Summary

Problems solved by technology

Nowadays, insect cell expression systems are commonly used to prepare VLPs, but for VLPs composed of multiple proteins, it is necessary to prepare baculoviruses expressing each protein, and then co-infect insect cells with these baculoviruses in different proportions to package the required VLPs. Recombinant baculovirus is essential for expression, and the mixed baculovirus in the product brings certain difficulties to later purification

In addition, the glycosylation of the insect cell expression system is still different from that of mammalian cells, which may affect the later immune effect

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