Biological composite oxygen producer and preparation method thereof
A composite oxygenate and oxygenate technology, which is applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problem of non-decomposition of oxygen-consuming substances at the bottom and the effect of probiotics Can not be reflected, the chemical oxygen consumption can not be reduced and other problems, to achieve the effect of alleviating the lack of oxygen in the pond, improving the micro-ecological balance, and increasing the oxygen effect
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Embodiment 1
[0045] Embodiment 1: Screening and preparation of chemical oxygenates
[0046] 1. Materials and methods
[0047] 1.1 Materials:
[0048] Reagents: anhydrous sodium carbonate, anhydrous sodium sulfate, 30% hydrogen peroxide, sodium silicate, salicylic acid, sodium tripolyphosphate, sodium chloride, stearic acid, polyethylene glycol 4000, carbon tetrachloride, Sodium percarbonate, the above reagents were purchased from Tianjin Kaitong Chemical Reagent Co., Ltd.; oxygen source, an oxygen-enhancing product produced by Shandong Baolai Lilai Bioengineering Co., Ltd.
[0049] Instruments: Portable Dissolved Oxygen Meter (Leici JPB-607A), Water Bath, Breeding Glass Cylinder (Barrel), Magnetic Stirrer, Watering Can.
[0050] 1.2 Method
[0051] 1.2.1 Preparation of basic oxygenate
[0052] ①The preparation of sodium percarbonate:
[0053] Spray 30% hydrogen peroxide evenly on anhydrous sodium carbonate in different proportions by spraying, and dry naturally in a ventilated place t...
Embodiment 2
[0083] The screening of embodiment 2 degrading ammonia nitrogen probiotics
[0084] 1 test material
[0085] Strains: 117 strains of Bacillus were provided by Shandong Baolai Lilai Bioengineering Co., Ltd. Institute of Biology;
[0086] Bacillus culture medium: 0.2% glucose, 1% peptone, 0.5% sodium chloride, 0.5% yeast extract, pH 7.0; all are percentages by mass;
[0087] Synthetic aquaculture wastewater: glucose 0.1g / L, K 2 HPO 4 0.75g / L, NaH 2 PO 4 0.25g / L, NaHCO 3 1g / L, MgSO 4 0.02g / L, NH 4 Cl 100mg / L (can be adjusted as needed), NaNO 2 100mg / L (can be adjusted as needed);
[0088] Breeding wastewater: glass tank fish farming wastewater, add NH 4 Cl 20mg / L, NaNO 2 10mg / L;
[0089] Sodium citrate was purchased from Tianjin Kaitong Chemical Reagent Co., Ltd.
[0090] 2 test method
[0091] 2.1 Determination of ammonia nitrogen - Nessler's reagent colorimetric method
[0092] 2.1.1 Reagents:
[0093] (1) Ammonium standard stock solution: Weigh 3.819g of ...
Embodiment 3
[0126] Embodiment 3: the preparation of biocomposite oxygenate
[0127] Formula: 2 parts of chemical oxygen enhancer, 1 part of biological agent, 0.1 part of sodium citrate, 0.8 part of adhesive;
[0128] The biological agent is bacterial powder of Bacillus subtilis N9-1-35 (Bacillus subtilis N9-1-35);
[0129] The binder is a paste obtained by mixing starch and water at a ratio of 1g:4mL.
[0130] Preparation:
[0131] (1) Preparation of chemical oxygenate: Spray hydrogen peroxide evenly on anhydrous sodium carbonate, stir continuously during this period, then add sodium chloride and mix well to form a crystalline compound, and grind the compound into powder after cooling. Promptly get the chemical oxygenation agent; the mass ratio of anhydrous sodium carbonate and hydrogen peroxide addition is 5:3, and the addition of sodium chloride is 10% of anhydrous sodium carbonate and hydrogen peroxide gross weight;
[0132] (2) Mix the chemical oxygenate prepared in step (1), the b...
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