Medical suppository for treating chronic nephrosis and preparation method thereof
A technology for chronic kidney disease and drugs, which is applied in drug combinations, pharmaceutical formulas, and medical raw materials derived from molluscs. It can solve the problems of patients with diarrhea, complicated operations, and patient pain, so as to improve absorption, avoid side effects, and have definite curative effects. Effect
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Embodiment 1
[0025] Weigh the following raw materials: rhubarb 6g, salvia miltiorrhiza 20g, Liuyuexue 20g, raw oyster 20g, tin leisan 6g.
[0026] Prepare as follows:
[0027] (1) Rhubarb is crushed, added in 120ml of 70% acetone aqueous solution, ultrasound is advanced 20min, extracted twice, and the extract is combined; the extract is concentrated under reduced pressure, acetone is reclaimed, filtered, and the filtrate is taken; the filtrate is placed on Sephadex LH- 20 gel chromatographic column, eluted with water, 30% methanol aqueous solution, 50% methanol aqueous solution, 70% methanol aqueous solution respectively, got 50% methanol aqueous solution and 70% methanol aqueous solution eluent; Press to recover methanol and water to obtain a thick paste, and microwave drying to obtain rhubarb dry extract;
[0028] (2) Pulverize salvia miltiorrhiza, Liuyuexue, raw oyster, reflux extraction with 1200ml of 80% ethanol, extract for 2 hours, filter, keep the dregs, collect the ethanol extrac...
Embodiment 2
[0032] Weigh the following Chinese medicinal materials: rhubarb 9g, salvia miltiorrhiza 24g, Liuyuexue 24g, raw oyster 24g, tin leisan 9g.
[0033] Prepare as follows:
[0034] (1) Rhubarb is pulverized, added in 180ml of 70% acetone aqueous solution, ultrasound is advanced 20min, extracted twice, and the extract is combined; the extract is concentrated under reduced pressure, acetone is recovered, filtered, and the filtrate is taken; the filtrate is placed on Sephadex LH- 20 gel chromatographic column, eluted with water, 30% methanol aqueous solution, 50% methanol aqueous solution, 70% methanol aqueous solution respectively, got 50% methanol aqueous solution and 70% methanol aqueous solution eluent; Press to recover methanol and water to obtain a thick paste, and microwave drying to obtain rhubarb dry extract;
[0035] (2) Grind the salvia miltiorrhiza, Liuyuexue, and raw oysters, extract with 1420ml of 80% ethanol under reflux, extract for 2 hours, filter, keep the dregs, c...
Embodiment 3
[0039] Weigh the following Chinese medicinal materials: rhubarb 12g, salvia miltiorrhiza 30g, Liuyuexue 30g, raw oyster 30g, tin leisan 10g.
[0040] Prepare as follows:
[0041] (1) Rhubarb is pulverized, added in 240ml of 70% acetone aqueous solution, ultrasound is advanced 20min, extracted twice, and the extract is combined; the extract is concentrated under reduced pressure, acetone is reclaimed, filtered, and the filtrate is taken; the filtrate is placed on Sephadex LH- 20 gel chromatographic column, eluted with water, 30% methanol aqueous solution, 50% methanol aqueous solution, 70% methanol aqueous solution respectively, got 50% methanol aqueous solution and 70% methanol aqueous solution eluent; Press to recover methanol and water to obtain a thick paste, and microwave drying to obtain rhubarb dry extract;
[0042] (2) Pulverize salvia miltiorrhiza, Liuyuexue, raw oyster, reflux extraction with 1800ml of 80% ethanol, extract for 2 hours, filter, keep the dregs, collect...
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